Urinary cystatin C as biomarker for identification of kidney disease in dogs

DOI: 10.18805/ijar.B-3481    | Article Id: B-3481 | Page : 196-199
Citation :- Urinary cystatin C as biomarker for identification of kidney disease in dogs.Indian Journal Of Animal Research.2019.(53):196-199
R. Sivasakthi, T. Satheesh Kumar, K. Padmanath, M. Chandrasekar, P. Sriram and V. Pandiyan mypands@rediffmail.com
Address : Department of Veterinary Biochemistry, Madras Veterinary College, Chennai-600 007, Tamil Nadu, India.
Submitted Date : 12-08-2017
Accepted Date : 17-10-2017

Abstract

The aim of the study was to identify urinary biomarker for early detection of renal dysfunction in dogs. Paired blood and urine samples were analysed from dogs classified on the basis of International Renal Interest Society (IRIS) staging system. Serum biochemistry revealed a highly significant increase in blood urea nitrogen and creatinine levels in dogs with kidney disease. Urinalysis also revealed a highly significant increase in the level of urinary protein and urinary protein creatinine (UPC) ratio in dogs with kidney disease. A highly significant decrease in urinary creatinine and specific gravity level was also present in all groups of dogs with kidney disease. The SDS-PAGE of urinary proteins obtained from dogs with renal failure revealed the presence of low molecular weight (LMW) proteins of 14.7 KDa. This protein was identified as cystatin C protein by Immunoblotting using anti-cystatin C antibody and it has been suggested as biomarker for the diagnosis of renal disease. 

Keywords

Cystatin C Low molecular weight proteins Renal failure

References

  1. Coles, E. H. (1986). Veterinary Clinical Pathology. 4thEdn, W. B. Saunders Company, Philadephia. 172-188.
  2. Conti, M., Moutereau, S., Zater, M., Lallali, K., Durrbach, A., Manivet, P., Eschwege, P., Loric, S. (2006). Urinary cystatin C as a specific marker of tubular dysfunction. Clinical Chemistry and Laboratory Medicine. 44: 288–291. 
  3. Forterre, S., Raila, J. and Schweigert, F.J.. (2004). Protein profiling of urine from dogs with renal disease using ProteinChip analysis. J Vet Diagn Investig. 16: 271–277.
  4. Huges, D. (1992). Polyuria and polydipsia. Comp Cont Edu Pract Vet. 14: 1161-1175.
  5. Jacob, F., Polzin D.J. and Osborne. C.A. (2005). Evaluation of the association between initial proteinuria and morbidity rate or death in dogs with naturally occurring chronic renal failure. JAVMA. 226: 393–400.
  6. Jeong, W. D. (2006). Canine renal failure syndrome in three dogs. J Vet Sci. 7 (3): 299-301.
  7. Laemmli U.K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227, 680-685.
  8. Lamb, E.J., MacKenzie F. and Stevens. P.E. (2009). How should proteinuria be detected and measured? Ann Clin Biochem. 46: 205-217.
  9. Littman, M. P. (2011). Protein-losing nephropathy in small animals. Vet Clin Small Anim Pract. 41: 31–62.
  10. Loor, J., Daminet, S., Smets, P., Maddens, B. and Meyer, E. (2013). Urinary biomarkers for acute kidney injury in dogs. J Vet Intern Med. 27: 998-1010.
  11. Lulich, J. P., Osborne C. A. and O’Brien. T. D. (1992). Feline renal failure: questions, answers, questions. Compend Contin Educ Pract Vet.14 (2): 127–53.
  12. Mayne, P.D. (1994). The kidneys and renal calculi. In: Clinical Chemistry in Diagnosis and Treatment, 6thed, Edward Arnold Publications, London. Pp. 2-24.
  13. Mehta, R.L., Kellum, J.A., Shah, S.V., Molitoris, B.A., Ronco, C., Warnock, D.G. and Levin, A. (2007). Acute Kidney Injury Network: report of an initiative to improve outcomes in acute kidney injury. Crit Care. 11: 31.
  14. Monti, P., Benchekroun, G., Berlato, B. and Archer, J. (2012). Initial evaluation of canine urinary cystatin C as a marker of renal tubular dysfunction. J Small Anim Pract. 53: 254-259.
  15. Nabity, M. B., Lees, G. E., Dangott, L. J., Cianciolo, R., Suchodolski, J. S. and Steiner. J. M. (2011). Proteomic analysis of urine from male dogs during early stages of tubulointerstitial injury in a canine model of progressive glomerular disease. Vet Clin Pathol. 40(2): 222–36.
  16. Park, M. Y., Choi, S. J., Kim, J. K., Hwang, S. D. and Lee, Y. W., (2013). Urinary cystatin C levels as a diagnostic and prognostic biomarker in patients with acute kidney injury. Nephrol. 18(4): 256-262.
  17. Polzin, D.J.(2011). Chronic kidney disease in small animals. Vet Clin North Am: Small Anim Pract. 41: 15–30.
  18. Radostitis, O.M., Blood, D.C., Gray, G.C. and Hinchcliff, K.W. (2008). Veterinary Medicine: A text book of cattle, sheep, pig, goat and horse. Bailliere Tindall, London, p. 1877.
  19. Satheeshkumar, T., Chandrasekar, M. and Pandiyan, V. (2016). Retinol binding protein as bio-marker for diagnosis of chronic kidney disease in dogs. Int J Adv Res. 4(3): 771-774.
  20. Snedecor, G.W and Cochran, W.G. (1994). Statistical Methods (Eighth edition). J Edu Behav Stat. 19(3): 304-307.
  21. Srikanth, K. and Karlapudi, S.K. (2015). Haemato-abiochemical changes in dogs with renal insufficiency and its diagnostic significance. Animal Science Reporter. 9, Issue 4.
  22. Thongboonkerd, V. (2005). Proteomic analysis of renal diseases: Unraveling the pathophysiology and biomarker discovery. Expert Rev Proteomics, 2: 349–366.

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