Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

  • Print ISSN 0367-6722

  • Online ISSN 0976-0555

  • NAAS Rating 6.50

  • SJR 0.263

  • Impact Factor 0.5 (2023)

Frequency :
Monthly (January, February, March, April, May, June, July, August, September, October, November and December)
Indexing Services :
Science Citation Index Expanded, BIOSIS Preview, ISI Citation Index, Biological Abstracts, Scopus, AGRICOLA, Google Scholar, CrossRef, CAB Abstracting Journals, Chemical Abstracts, Indian Science Abstracts, EBSCO Indexing Services, Index Copernicus
Indian Journal of Animal Research, volume 53 issue 1 (january 2019) : 37-40

Effect of trehalose- an impermeant cryoprotectant on cryopreservation of Black Bengal buck semen

M. Karunakaran, Pangdun Konyak, Ajoy Mandal, Mohan Mondal, C. Bhakat, S. Rai, M.K. Ghosh, R. Behera
1ICAR- National Dairy Research Institute Eastern Regional Station, Kalyani-741 235, West Bengal, India
Cite article:- Karunakaran M., Konyak Pangdun, Mandal Ajoy, Mondal Mohan, Bhakat C., Rai S., Ghosh M.K., Behera R. (2018). Effect of trehalose- an impermeant cryoprotectant on cryopreservation of Black Bengal buck semen. Indian Journal of Animal Research. 53(1): 37-40. doi: 10.18805/ijar.B-3470.
The experiment was conducted to study the effect of trehalose an impermeant extra cellular cryoprotectant supplementation on cryopreservation of Black Bengal buck semen. Semen ejaculates (n = 24) were diluted in Tris- soybean lecithin - glycerol extender having trehalose @ 0, 50,100and 150 mM. Samples were equilibrated at 5°C for 3 hrs, frozen and stored in liquid nitrogen. Semen samples were evaluated for sperm motility,functional membrane integrityand concentration of lipid peroxide compound malondialdehyde at three stages such as 10 minsafter dilution with extender, after completion of equilibration period and after freeze thawing. It was found that the control group (0mM trehalose)had significantly better in vitro sperm characters when compared to extenders supplemented with trehalose (50 mM , 100 mM and 150 mM) during different stages of cryopreservation. It can be concluded that supplementation of trehaloseas a cryoprotectant did not improve the cryopreservability of Black Bengal buck semen. 
  1. Aboagla, E.M.E.and Terada T. (2003). Trehalose-enhanced fluidity of the goat sperm membrane and its protection during freezing. Biol. Reprod.,69: 1245-1250.
  2. Aisen, E.G., MedinaV.H. and Venturino A. (2002). Cryopreservation and post-thawed fertility of ram semen frozen in different trehalose concentrations. Theriogenology, 57:1801-1808.
  3. Atessahin, A., Bucak M.N., TuncerP.B. and Kizil M. (2008). Effects of anti-oxidantadditives on microscopic and oxidative parameters of Angora goat semen following the freezethawing process. Small Rumin Res.,77(1): 38-44.
  4. Bucak,M.N., Ateººahin A., Variºli O., Yüce A., Tekin N. and Akçay A. (2007). The influence of trehalose, taurine, cysteamine and hyaluronan on ram semen: microscopic and oxidative stress parameters after the freeze–thawing process.Theriogenology, 67:1060-1067.
  5. Chen, Y., Foote R.H., Tobback L., Zhang L. and Hough S. (1993). Survival of bull spermatozoa seeded and frozen at different rates in egg yolk-Tris and whole milk extenders. J. Dairy Sci.,76:1028-1034.
  6. Garner, D. (1991). Artificial insemination, In: Reproduction in Domestic Animals.[P.T. Cupps (Ed.)]. Academic Press, San Diego251–278.
  7. Hammerstedt, R.H. and GrahamJ.K.(1992). Cryopreservation of poultry sperm: The enigma of glycerol. Cryobiology, 29:26-38.
  8. Hu, J.H.,Zan L.S., ZhaoX.L., Li Q.W., Jiang Z.L, Li Y.K. and LiX.(2010). Effects of trehalose supplementation on semen quality and oxidative stress variables in frozen–thawed bovine semen. J Anim Sci., 88:1657–1662.
  9. Jafaroghli, M., Khalili B., Farshad A. and Zamiri M.J. (2011). The effect of supplementation of cryopreservation diluents with sugars on the post-thawing fertility of ram semen. Small Rumin Res.,96: 58-63.
  10. Karunakaran, M. and Devanathan T. G. (2016a). Evaluation of bull semen for fertility-associated protein, in vitro characters and fertility. J. Appl. Anim. Res.,45(1): 136- 144. 
  11. Karunakaran, M., Chakurkar E. B.,Ratnakaran U.,Naik P. K.,Mondal M.,Mondal A. and Singh N. P. (2016). Characteristics of boar semen preserved at liquid state. J. Appl. Anim. Res.,45 (1): 217–220. 
  12. Khalili B, Farshad A, Zamiri MJ, Rashidi A and Fazeli P. 2009. Effects of sucrose and trehalose on the freezability of Markhoz goat spermatozoa. Asian-Australasian Journal Animal Science 22:1614- 1619.
  13. Khandoker, M. A., Apu A. S., HusainS. S. and NotterD. R.(2011). A baseline survey on the availability of Black Bengal breeding bucks in different districts of Bangladesh. J. Bangladesh Agril. Univ., 9(1): 91–96.
  14. Kumar, D., Jhamb D. and Saxena A. (2011). Effect of different seasons and thawing protocols on Certain seminal attributes of indian standard bredStallion semen preserved using 1% glycerol and 1% dimethyl formamide as cryoprotectant. Indian J. Anim. Res., 45 (4): 247 – 255. 
  15. Najafi, A., Zhandi M., Towhidi A., Sharafi M., Akbari S.A., KhodaeiM. M. and Martinez-Pastor F. (2013). Trehalose and glycerol have a dose-dependent synergistic effect on the post-thawing quality of ram semen cryopreserved in a soybean lecithin-based extender. Cryobiology, 66:275–282.
  16. Purdy. P. (2006). A review on goat sperm cryopreservation. Small Rumin Res., 63:215–225.
  17. Reddy,N.S.S., MohanaraoG.J. and AtrejaS.K.(2010). Effects of adding taurine and trehalose to a tris-based egg yolk extender on buffalo (Bubalus bubalis) sperm quality following cryopreservation. Anim Reprod Sci.,119(3-4):183-90. 
  18. Suleiman, S.A., Ali M.E., Zaki M.S., Malik E.M.E.A. and Nast M.A. (1996). Lipid peroxidation and human sperm motility protective role of vitamin E. J. Androl.,17(5): 530–537.
  19. Tuncer, P.B.,Umut T.D., SerhatB., TanerO., ErdemC.K. and Halil E.(2013). Effects of different doses of trehalose supplementation in egg yolk extender in frozen–thawed Angora buck semen. Small Rumin Res.,113:383-389.
  20. Uysal Oand Bucak MN.2009. Therole of different trehalose concentrations and cooling rates in freezing of ram semen. Ankara Üniversitesi Veteriner Fakültesi Dergisi,56: 99-103. 
  21. Woelders H, Matthij A and Engel B. 1997. Effects of trehalose and sucrose, osmolality of the freezing medium and cooling rate on viability and intactness of bull sperm after freezing and thawing. Cryobiology,35:93-105. 

Editorial Board

View all (0)