Indian Journal of Animal Research

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Research Article

Indian Journal of Animal Research, volume 53 issue 1 (january 2019) : 37-40

Effect of trehalose- an impermeant cryoprotectant on cryopreservation of Black Bengal buck semen

M. Karunakaran, Pangdun Konyak, Ajoy Mandal, Mohan Mondal, C. Bhakat, S. Rai, M.K. Ghosh, R. Behera
1ICAR- National Dairy Research Institute Eastern Regional Station, Kalyani-741 235, West Bengal, India
Cite article:- Karunakaran M., Konyak Pangdun, Mandal Ajoy, Mondal Mohan, Bhakat C., Rai S., Ghosh M.K., Behera R. (2018). Effect of trehalose- an impermeant cryoprotectant on cryopreservation of Black Bengal buck semen. Indian Journal of Animal Research. 53(1): 37-40. doi: 10.18805/ijar.B-3470.

ABSTRACT

The experiment was conducted to study the effect of trehalose an impermeant extra cellular cryoprotectant supplementation on cryopreservation of Black Bengal buck semen. Semen ejaculates (n = 24) were diluted in Tris- soybean lecithin - glycerol extender having trehalose @ 0, 50,100and 150 mM. Samples were equilibrated at 5°C for 3 hrs, frozen and stored in liquid nitrogen. Semen samples were evaluated for sperm motility,functional membrane integrityand concentration of lipid peroxide compound malondialdehyde at three stages such as 10 minsafter dilution with extender, after completion of equilibration period and after freeze thawing. It was found that the control group (0mM trehalose)had significantly better in vitro sperm characters when compared to extenders supplemented with trehalose (50 mM , 100 mM and 150 mM) during different stages of cryopreservation. It can be concluded that supplementation of trehaloseas a cryoprotectant did not improve the cryopreservability of Black Bengal buck semen. 

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