Indian Journal of Animal Research

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Research Article

Indian Journal of Animal Research, volume 50 issue 4 (august 2016) : 466-470

Changes in expression of Th1 and Th2 cytokines in bovine peripheral blood mononuclear cells during early pregnancy

Ling Yang, Yongxiang Wang, Xingshu Ma, Songping Wang, Leying Zhang*
1<p>Department of Animal Science, College of Agriculture, Hebei University of Engineering, Handan-056 021, China.</p>
Cite article:- Yang Ling, Wang Yongxiang, Ma Xingshu, Wang Songping, Zhang* Leying (2015). Changes in expression of Th1 and Th2 cytokines in bovine peripheral blood mononuclear cells during early pregnancy . Indian Journal of Animal Research. 50(4): 466-470. doi: 10.18805/ijar.5538.

ABSTRACT

Conceptus-derived signals are involved in the maternal systemic immune regulation through blood circulation and lymph circulation by peripheral blood mononuclear cells (PBMC) in cattle. There exists a Th2-biased response in maternal uterus during early pregnancy in cattle. However, it is unclear that the changes in expression of Th1 and Th2 cytokines in PBMC during early pregnancy in cows. At present study, the expression of Th1 and Th2 cytokines in PBMC were explored at day 18 non-pregnant cows, day 18 and day 30 pregnant cows. Our results showed that IFN-g was attenuated in PBMC, but TNF-a and IL-2 in PBMC were up-regulated during early pregnancy in cows. However, the member of the Th2 cytokines, including IL-4, IL-5, IL-6, IL-10 and IL-13, was augmented in day 30 pregnant PBMC. In conclusion, there existed a Th2-biased response in PBMC during early pregnancy, which was essential for successful pregnancy in cattle.

REFERENCES


  1. Blitek, A., Morawska, E. and Ziecik, A.J. (2012). Regulation of expression and role of leukemia inhibitory factor and interleukin-6 in the uterus of early pregnant pigs. Theriogenology, 78: 951-564. 

  2. Cantón, G.J., Katzer, F., Maley, S.W., Bartley, P.M, Benavides-Silván, J., Palarea-Albaladejo, J., Pang, Y., Smith, S.H., Rocchi, M., Buxton, D., Innes, E.A. and Chianini, F. (2014). Cytokine expression in the placenta of pregnant cattle after inoculation with Neospora caninum. Vet. Immunol. Immunopathol., 161: 77-89.

  3. Fair, T. (2015). The contribution of the maternal immune system to the establishment of pregnancy in cattle. Front Immunol., 6:7. 

  4. Goyal, P., Brünnert, D., Ehrhardt, J., Bredow, M., Piccenini, S. and Zygmunt, M. (2013). Cytokine IL-6 secretion by trophoblasts regulated via sphingosine-1-phosphate receptor 2 involving Rho/Rho-kinase and Rac1 signaling pathways. Mol. Hum. Reprod., 19: 528-38.

  5. Hu, W.T., Huang, L.L., Li, M.Q., Jin, L.P., Li, D.J. and Zhu, X.Y. (2015). Decidual stromal cell-derived IL-33 contributes to Th2 bias and inhibits decidual NK cell cytotoxicity through NF-êB signaling in human early pregnancy. J. Reprod. Immunol., 109: 52-65.

  6. Liao, W., Lin, J.X. and Leonard, W.J. (2011). IL-2 family cytokines: new insights into the complex roles of IL-2 as a broad regulator of T helper cell differentiation. Curr. Opin. Immunol., 23: 598-604.

  7. Liao, W., Lin, J.X. and Leonard, W.J. (2013). Interleukin-2 at the crossroads of effector responses, tolerance, and immunotherapy. Immunity, 38: 13-25.

  8. Lin, Y.L., Liang, Y.C. and Chiang, B.L. (2007). Placental growth factor down-regulates type 1 T helper immune response by modulating the function of dendritic cells. J. Leukoc. Biol., 82: 1473-1480.

  9. Maeda, Y., Ohtsuka, H., Tomioka, M. and Oikawa, M. (2013). Effect of progesterone on Th1/Th2/Th17 and regulatory T cell-related genes in peripheral blood mononuclear cells during pregnancy in cows. Vet. Res. Commun., 37: 43-49.

  10. Martínez, F.F., Knubel, C.P., Sánchez, M.C., Cervi, L. and Motrán, C.C. (2012). Pregnancy-specific glycoprotein 1a activates dendritic cells to provide signals for Th17-, Th2-, and Treg-cell polarization. Eur. J. Immunol., 42: 1573-1584. 

  11. Ott, T.L. and Gifford, C.A. (2010). Effects of early conceptus signals on circulating immune cells: lessons from domestic ruminants. Am. J. Reprod. Immunol., 64: 245-254.

  12. Sakumoto, R., Hayashi, K.G. and Takahashi, T. (2014). Different expression of PGE synthase, PGF receptor, TNF, Fas and oxytocin in the bovine corpus luteum of the estrous cycle and pregnancy. Reprod. Biol., 14: 115-121.

  13. Shirasuna, K., Matsumoto, H., Kobayashi, E., Nitta, A., Haneda, S., Matsui, M., Kawashima, C., Kida, K., Shimizu, T. and Miyamoto, A. (2012). Upregulation of interferon-stimulated genes and interleukin-10 in peripheral blood immune cells during early pregnancy in dairy cows. J. Reprod. Dev., 58: 84-90.

  14. Sokol, C.L., Barton, G.M., Farr, A.G. and Medzhitov, R. (2008). A mechanism for the initiation of allergen-induced T helper type 2 responses. Nat. Immunol., 9: 310-318. 

  15. Spencer T.E. (2013). Early pregnancy: Concepts, challenges, and potential solutions. Anim. Front., 3: 48-55.

  16. Szóstek A.Z., Adamowski, M., Galvão, A.M., Ferreira-Dias, G.M. and Skarzynski, D.J. (2014). Ovarian steroid-dependent tumor necrosis factor-á production and its action on the equine endometrium in vitro. Cytokine, 67: 85-91.

  17. Szóstek AZ, Siemieniuch, M.J., Deptula, K., Woclawek-Potocka, I. , Majewska, M., Okuda, K. and Skarzynski, D.J. (2011). Ovarian steroids modulate tumor necrosis factor-á and nitric oxide-regulated prostaglandin secretion by cultured bovine oviductal epithelial cells. Domest. Anim. Endocrinol., 41: 14-23.

  18. Tuo, W., MacMillan, H., Günter, N., Bazer, F.W. and Brown, W.C. (1999). Upregulation of interleukin-4 and IFN-gamma expression by IFN-tau, a member of the type I IFN family. J. Interferon Cytokine Res., 19: 179-187.

  19. Wong, M.L. and Medrano, J.F. (2005). Real-time PCR for mRNA quantita­tion. Biotechniques, 39: 75-85.

  20. Wyckoff, J.H. 3rd, Howland, J.L., Scott, C.M., Smith, R.A. and Confer, A.W. (2005). Recombinant bovine interleukin 2 enhances immunity and protection induced by Brucella abortus vaccines in cattle. Vet. Microbiol., 111: 77-87.

  21. Yang, L., Zhang, L.Y., Qiao, H.Y., Liu, N., Wang, Y.X. and Li, S.J. (2014). Maternal immune regulation by conceptus during early pregnancy in the bovine. Asian J. Anim. Vet. Adv., 9: 610-620.

  22. Zhang, L., Xia, Y., Tang, F., Li, S.J., Yang, L. and Wang, B. (2015). The regulation of intrauterine immune cytokines and chemokines during early pregnancy in the bovine. Large Anim. Rev., 21: 23-31.

  23. Zurawski, G. and de Vries J.E. (1994). Interleukin 13, an interleukin 4-like cytokine that acts on monocytes and B cells, but not on T cells. Immunol. Today, 15: 19-26.
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