Effect of microbial proteases on the performance and carcass traits in commercial broilers fed  maize-soy bean meal -meat cum bone meal based diets

DOI: 10.18805/ijar.B-3465    | Article Id: B-3465 | Page : 89-93
Citation :- Effect of microbial proteases on the performance and carcass traits in commercial broilers fed maize-soy bean meal -meat cum bone meal based diets.Indian Journal Of Animal Research.2019.(53):89-93
T. Srilatha, V. Ravinder Reddy, V. ChinniPreetam , S.V. Rama Rao and Y. Ramana Reddy srilatha.mangalam@gmail.com
Address : Department of Poultry Science, College of Veterinary Science, P.V. Narasimha Rao Telangana Veterinary University, Rajendranagar, Hyderabad-500 030, Telangana, India.
Submitted Date : 14-07-2017
Accepted Date : 6-10-2017


An experiment was conducted to evaluate the effective combinations of proteases (acid, neutral and alkaline) and their concentrations on performance and carcass traits in commercial broilers fed on diets containing sub-optimal protein levels. A total of 320 broilers were used in completely randomized design with eight treatments with eight replicates in each treatment and 5chicks in each replicate.  Maize-soybean meal (CD) and maize-soybean-meat cum bone meal (BD) based  diets  were formulated to contain 23, 21 and 19.5% crude protein (CP) and 19.5,18 and 17.5% CP, respectively during pre-starter (1-11 d), starter (12-28d) and finisher (29-42d) phases. All the diets were fed adlibitum from 1d to 42 d of age.  The low protein BD were supplemented with two different combinations of proteases (1:1:1 and 2:1:1 acidic, neutral and alkaline proteases, respectively) with total activity of 2000, 4000 and 6000u/kg.The results revealed that the broilers fed CD had significantly higher body weight gain compared to those fed BD. Supplementation of proteases to the BD significantly (P < 0.05) improved the body weight gain and feed efficiency during 21 d of age, but not during the latter phase ( 42 d of age) of growth. The broilers fed on CD had significantly (P < 0.05) higher ready to cook and breast yields compared to those fed the BD or BD with protease supplementation. The relative weight of abdominal fat was significantly (P < 0.05) reduced in broilers fed BD with 1:1:1 (acidic, neutral and alkaline proteases) at 4000 u protease / kg diet as compared to BD. It is suggested that addition of proteases to low protein diets had a significant positive effect in young chicken fed on maize-soybean meal and -meat and bone meal based diets.


Body weight gain Broilers Crude protein Feed efficiency Proteases.


  1. AOAC. (2000). Official Methods of Analysis. 17th ed. Association of Official Analytical Chemists, Gaithersburg, MD.
  2. Ajayi,H.L. (2015).Effect of protease supplementation on performance and carcass weights of broiler chickens fed low protein diets,Nigerian Journal of Agriculture, Food and Environment. 11(1):29-32
  3. Cowieson, A.J and Roos, F.F.,( 2014). Bioefficacy of a mono-component protease in the diets of pigs and poultry: a meta-analysis of effect on ileal amino acid digestibility. Journal of Applied Animal Nutrition 2: e13-e20.
  4. Duncan D B (1955) Multiple ‘F’ test.Biometrics 11: 142.
  5. Faria Filho D E (2003) Efeito de dietas com baixoteorprotéico, formuladasusando o conceito de proteína ideal, para frangos de cortecriadosemtemperaturasfria, termoneutra e quente. [Dissertação].Jaboticabal (SP): UniversidadeEstadualPaulista.
  6. Freitas, D.M., Vieira, S.L., Angel, C.R., Favero, A., Maiorka, A., (2011). Performance and nutrient utilization of broilers fed diets supplemented with a novel mono-component protease. Journal of Applied Poultry Research20:322-334.
  7. Ghazi S, Rooke J A, Galbraith H and Bedford M R (2002) The potential for the improvement in the nutritive value of soybean meal by different proteases in broiler chicks and broiler cockerels. British Poultry Science43:70–77.
  8. Jiang Q, Waldroup P W and Fritts C A (2005) Improving the utilization of diets low in crude protein for broiler chickens. 1. Evaluation of special amino acid supplementation to diets low in crude protein. International Journal of Poultry Science4:115122.
  9. Kamran M, AslamMirza, Ahsan-ul-Haq and Mahmood S (2004)Effect of decreasing dietary protein levels with optimal amino acids profile on the performance of broilers. Pakistan Veterinary Journal24(4): 75 – 78.
  10. Maiorka A, Favero A, Meurer R F P, Moraes M T T and Sorbara J O B (2009) Broiler chicken performance and ileal digestibility is improved by a protease used in corn/soybean meal/meat bone meal diet. International Poultry Science Forum , Atlanta GA. Abstr. p. 33.
  11. McGill E R (2009) Effects Of Low Crude Protein Diets With Amino Acid Supplementation On Broiler Performance In The Starter Period. Chapter 4 in: Master of science Dissertation, University of Missouri, Columbia, MO.
  12. National Research Council 1994 Nutrient Requirements of Poultry. 9th Rev. Ed.National Academy Press, Washington, D.C.
  13. Nitsan Z, Duntington E A and Siegel P B (1991) Organ growth and digestive enzyme levels to fifteen days of age in lines of chicken differing in body weight. Poultry Science70:2040-2048
  14. Noy Y and Sklan D (1995) Digestion and absorption in the young chick, Poultry Science74: 366-373.
  15. Ravindran V, Hew L I, Ravindran G and Bryden W L( 2005) Apparent ileal digestibility of amino acid in dietary ingredients for broiler chickens. Animal Science 81:85-97.
  16. Rosa A P, Scher A, Stefanello, Diaz E and Sarbara O B( 2009) Effect of pure protease enzyme and dietary protein/amino acid levels on broiler performance. International Poultry Science Forum, Atlanta GA. Abstr. p. 32.
  17. Si J, Fritts C A, Burnham D J and Waldroup P W( 2004) Extent to which crude protein may be reduced in corn soybean meal broiler diets through amino acid supplementation. International Journal of Poultry Science 3 (1):4650.
  18. Uni Z, Noy Y and Sklan D (1999) Posthatch development of small intestinal function in the poult. Poultry Science78:215–222.
  19. Wang X and Parsons C M (1998) Effect of raw material source, processing system, and processing temperatures on amino acid digestibility of meat and bone meals. Poultry Science 77: 834-841
  20. Yadav J L and SahR A (2005)Supplementation of corn-soybean based broiler’s diets with different levels of acid protease. Journal of the Institute of Agriculture and Animal Sciences 26:65-70.
  21. Yan.F, Garribay.I,. Arce, Lopez-coello, Camacho.d, Disbennet.P, Vazquez-anon, Manangi,andOdetallah.n(2012) Effects of protease supplementation on broiler performance and in vitro protein digestibility. 23rd Annual Australian Poultry Science Symposium Sydney, New South Wales.Pg:135

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