Effect of mineral supplementation on humoral immunity against rabies vaccine in dog pups

DOI: 10.18805/ijar.B-3433    | Article Id: B-3433 | Page : 615-618
Citation :- Effect of mineral supplementation on humoral immunity against rabies vaccine in dog pups.Indian Journal of Animal Research.2018.(52):615-618
Sarita Devi, M.C. Sharma, R.P. Singh, U. Dimri, A.C. Patel, Pankaj Kumar and R.D. Singh drsarita17@yahoo.co.in
Address : Division of Medicine, Indian Veterinary Research Institute (IVRI), Izatnagar-243 122, Uttar Pradesh, India.
Submitted Date : 5-05-2017
Accepted Date : 4-07-2017


The present study was aimed to determine the effect of a specially formulated mineral supplement in shaping the humoral immune response of dog pups in response to anti-rabies vaccination. Mineral supplement was formulated based on plasma analyzed for macro and micro minerals randomly collected from 226 dogs. Twelve mongrel/non-descript dog pups (3 – 6 months) randomly allocated into two groups (n=6) were tested for stimulation of humoral immune system by assessing the antibody titre against rabies vaccine using Rapid Fluorescent Focus Inhibition Test (RFFIT) and in vivo Mouse Neutralization Test (MNT) for antibody detection post supplementation of formulated mineral supplement upto 28 days. Early and higher antibody titre (>1:4096) was recorded on day seven itself in the group of dog pups fed with formulated mineral supplemented as compared to non-supplemented group. Present study indicated that mineral supplementation prior to the anti-rabies vaccination may elicit quick and high level of protective antibodies. The findings may also be important in the event of post bite immunization, in combination with this mineral supplementation for quick protective antibody response.


Dog pups Humoral immunity Mineral supplement Rabies vaccine Rapid fluorescent focus inhibition test


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