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Current IssueEditorial BoardOnline First ArticlesArchive

Research Article

volume 52 issue 1 (january 2018) : 99-104,   Doi: 10.18805/ijar.B-3411

Rotavirus A associated pathology of intestine and mesenteric lymph nodes and  occurrence in bovine calves of Gwalior and Bareilly regions

G
Gayatri Kashyap
R
R. Singh
Y
Y.S. Malik
R
R.K. Agrawal
K
K.P. Singh
P
P. Kumar
R
Rahul Singh
D
D. Gupta.
1Division of Pathology, ICAR- Indian Veterinary Research Institute, Izatnagar-243 122, Uttar Pradesh, India.
Cite article:- Kashyap Gayatri, Singh R., Malik Y.S., Agrawal R.K., Singh K.P., Kumar P., Singh Rahul, Gupta. D. (2018). Rotavirus A associated pathology of intestine and mesenteric lymph nodes and occurrence in bovine calves of Gwalior and Bareilly regions. Indian Journal of Animal Research. 52(1): 99-104. doi: 10.18805/ijar.B-3411.

ABSTRACT

To understand the pathology of natural cases of rotavirus (RVA) in bovine calves, a total of 40 cases below 6 months died due to diarrhoea were studied, out of which 7 cases (17.5%) turned positive for RVA by RT-PCR. Histopathology of small intestine showed loss of villous enterocytes, blunting and fusion of villi, elongation of crypts and mononuclear cells infiltration in the lamina-propria. The mesenteric lymph nodes were severely depleted of lymphocytes. These changes were corroborated with presence of RVA antigen in sections by dFAT and nucleic acid by RT-PCR. The fluorescent signals were more in mesenteric lymph nodes than in intestine. Besides, 115 rectal fecal samples were also collected from calves for RVA detection by RT-PCR using VP6 gene specific sets of primers. Dead carcasses of calves (n= 40) belonged to organized dairy farm of Bareilly, while rectal fecal samples belonged to both organized (n= 38) and unorganized farms (n= 77) of Bareilly and Gwalior. The overall occurrence of RVA was 19.3% (30/155), comprising 5/37 cases (13.5%) from Gwalior (MP) and 25/118 cases (21.1%) from Bareilly (UP). These findings suggest the infection of RVA widely prevalent in calves and have potential to escape from the intestinal site to mesenteric lymph nodes.

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Indian Journal of Animal Research
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