Banner
Current IssueEditorial BoardOnline First ArticlesArchive
Current IssueEditorial BoardOnline First ArticlesArchive

Research Article

volume 52 issue 9 (september 2018) : 1243-1247,   Doi: DOI:10.18805/ijar.v0iOF.9132

Cytogenetic Profile of Rajapalayam Dog Breed of Southern India

K
K.N Raja
R
R Saravanan
P
P Devendran
P
Pramod Kumar Singh
A
Anil Kumar Mishra
I
Indrajit Ganguly
1Animal Genetic Resources Division, ICAR-National Bureau of Animal Genetic Resources, Karnal-132001, Haryana, India
Cite article:- Raja K.N, Saravanan R, Devendran P, Singh Kumar Pramod, Mishra Kumar Anil, Ganguly Indrajit (2017). Cytogenetic Profile of Rajapalayam Dog Breed of Southern India. Indian Journal of Animal Research. 52(9): 1243-1247. doi: DOI:10.18805/ijar.v0iOF.9132.

ABSTRACT

Rajapalayam, an important dog breed of southern India which are maintained for guarding of farm houses and protection from wild animals. The aim of the present investigation is to study the karyology of this important dog population for cytological profiling. Metaphase plates were prepared after culturing of lymphocytes isolated from heparinized blood collected from animals of both the sexes. Giemsa banding, centromeric index, arm ratio and relative length were estimated through standard protocol. The cytogenetic profile of Rajapalayam dog is different from that of Chinese Raccoon and Japanese Raccoon dogs which belongs to Canidae family with a basic chromosome number of 78 without B chromosome. The X chromosomes are longer in bitches than that of in dogs. Giemsa banding analysis revealed higher number of bands (272) distributed among the 38 chromosome in Indian dog breed as compared to that Chinese Raccoon dog. The results of the present study gave insight knowledge to the researchers, dog breeders and kennel clubs about the karyology of Indian dog breeds and to know about any chromosomal abnormalities which may leads to fertility, growth and phenotypic abnormality related problems in this dog breed.   

REFERENCES

  1. Barch M J, Lawee H J and Arsham M S. (1991). Peripheral blood culture. In: The ACT cytogenetics Laboratory Manual, [Barch, M.J. (Ed.)] 2nd edition Newyork, Raven Press. pp. 17-30. 
  2. Ma¨kinen Auli, Majra-Terttu Kuokkanen and Maija Valtonen. (1986). A chromosome-banding study in the Finnish and the Japanese Raccoon dog. Hereditas 10: 97-105.
  3. Moorhead P S, Nowell P C, Mellman WJ , Battips D M and Hungerford D.A. (1960). Chromosome preparation of leukocytes cultured from human peripheral blood. Exp Cell Res 20: 613-616. 
  4. Pieñkowska A, Szczerbal I, Mäkinen A and Œwitoñski, M. (2002). G/Q-banded chromosome nomenclature of the Chinese Raccoon dog, Nyctereutes procyonoides procyonoides Gray. Hereditas 137: 75-78.
  5. Sumner A T, Evans H Tand Buckland R A. (1971). New technique for distinguishing between human chromosomes. Nature N B 232: 31-32. 
  6. Suryawanshi A R, Kanadkhedkar H L and Umrikar U D. (2004). Chromosome analysis of Domestic Dogs. Indian Vet J 81: 1071-1073.
  7. Switonski M, Rogalska-Niznik N, Szczerbal I and Baer M. (2003). Chromosome polymorphism and karyotype evolution of four canids: the dog, red fox, arctic fox and raccoon dog. Caryologia. 56 (4): 375-385.
  8. Topashka-Ancheva M, Gerasimova Ts, Dinchev V and Dimitrov K. (2009). Karyological data about the Bulgarian native dog breed “Karakachan Dog”. Biotechnol Biotec Eq 23: sup1, 136-139, DOI: 10.1080/13102818.2009.10818384.
  9. Wada M Y and Imai H T. (1991). On the Robertsonian polymorphism found in the Japanese Raccoon dog (Nyctereutes procyonoides viverrinus). Jpn J Genet 66: 1–11.
  10. Wada M Y, Suzuki T and Tsuchiya K. (1998). Re-examination of the chromosome homology between two subspecies of Japanese Raccoon dogs (Nyctereutes procyonoides albus and N.p. viverrinus). Caryologia 51 (1): 13-18.
Published In
Indian Journal of Animal Research
Article Metrics
Metrics Icon
0
Views
0
Citations
Reviewed By
Share Article
Download Article
In this Article
    Contact us
    Follow us

    Research Article

    volume 52 issue 9 (september 2018) : 1243-1247,   Doi: DOI:10.18805/ijar.v0iOF.9132

    Cytogenetic Profile of Rajapalayam Dog Breed of Southern India

    K
    K.N Raja
    R
    R Saravanan
    P
    P Devendran
    P
    Pramod Kumar Singh
    A
    Anil Kumar Mishra
    I
    Indrajit Ganguly
    1Animal Genetic Resources Division, ICAR-National Bureau of Animal Genetic Resources, Karnal-132001, Haryana, India
    Cite article:- Raja K.N, Saravanan R, Devendran P, Singh Kumar Pramod, Mishra Kumar Anil, Ganguly Indrajit (2017). Cytogenetic Profile of Rajapalayam Dog Breed of Southern India. Indian Journal of Animal Research. 52(9): 1243-1247. doi: DOI:10.18805/ijar.v0iOF.9132.

    ABSTRACT

    Rajapalayam, an important dog breed of southern India which are maintained for guarding of farm houses and protection from wild animals. The aim of the present investigation is to study the karyology of this important dog population for cytological profiling. Metaphase plates were prepared after culturing of lymphocytes isolated from heparinized blood collected from animals of both the sexes. Giemsa banding, centromeric index, arm ratio and relative length were estimated through standard protocol. The cytogenetic profile of Rajapalayam dog is different from that of Chinese Raccoon and Japanese Raccoon dogs which belongs to Canidae family with a basic chromosome number of 78 without B chromosome. The X chromosomes are longer in bitches than that of in dogs. Giemsa banding analysis revealed higher number of bands (272) distributed among the 38 chromosome in Indian dog breed as compared to that Chinese Raccoon dog. The results of the present study gave insight knowledge to the researchers, dog breeders and kennel clubs about the karyology of Indian dog breeds and to know about any chromosomal abnormalities which may leads to fertility, growth and phenotypic abnormality related problems in this dog breed.   

    REFERENCES

    1. Barch M J, Lawee H J and Arsham M S. (1991). Peripheral blood culture. In: The ACT cytogenetics Laboratory Manual, [Barch, M.J. (Ed.)] 2nd edition Newyork, Raven Press. pp. 17-30. 
    2. Ma¨kinen Auli, Majra-Terttu Kuokkanen and Maija Valtonen. (1986). A chromosome-banding study in the Finnish and the Japanese Raccoon dog. Hereditas 10: 97-105.
    3. Moorhead P S, Nowell P C, Mellman WJ , Battips D M and Hungerford D.A. (1960). Chromosome preparation of leukocytes cultured from human peripheral blood. Exp Cell Res 20: 613-616. 
    4. Pieñkowska A, Szczerbal I, Mäkinen A and Œwitoñski, M. (2002). G/Q-banded chromosome nomenclature of the Chinese Raccoon dog, Nyctereutes procyonoides procyonoides Gray. Hereditas 137: 75-78.
    5. Sumner A T, Evans H Tand Buckland R A. (1971). New technique for distinguishing between human chromosomes. Nature N B 232: 31-32. 
    6. Suryawanshi A R, Kanadkhedkar H L and Umrikar U D. (2004). Chromosome analysis of Domestic Dogs. Indian Vet J 81: 1071-1073.
    7. Switonski M, Rogalska-Niznik N, Szczerbal I and Baer M. (2003). Chromosome polymorphism and karyotype evolution of four canids: the dog, red fox, arctic fox and raccoon dog. Caryologia. 56 (4): 375-385.
    8. Topashka-Ancheva M, Gerasimova Ts, Dinchev V and Dimitrov K. (2009). Karyological data about the Bulgarian native dog breed “Karakachan Dog”. Biotechnol Biotec Eq 23: sup1, 136-139, DOI: 10.1080/13102818.2009.10818384.
    9. Wada M Y and Imai H T. (1991). On the Robertsonian polymorphism found in the Japanese Raccoon dog (Nyctereutes procyonoides viverrinus). Jpn J Genet 66: 1–11.
    10. Wada M Y, Suzuki T and Tsuchiya K. (1998). Re-examination of the chromosome homology between two subspecies of Japanese Raccoon dogs (Nyctereutes procyonoides albus and N.p. viverrinus). Caryologia 51 (1): 13-18.
    In this Article
      Contact us
      Follow us
      Published In
      Indian Journal of Animal Research

      Editorial Board

      View all (0)