Cytogenetic Profile of Rajapalayam Dog Breed of Southern India

DOI: DOI:10.18805/ijar.v0iOF.9132    | Article Id: B-3369 | Page : 1243-1247
Citation :- Cytogenetic Profile of Rajapalayam Dog Breed of Southern India.Indian Journal Of Animal Research.2018.(52):1243-1247
K.N Raja, R Saravanan, P Devendran, Pramod Kumar Singh, Anil Kumar Mishra and Indrajit Ganguly drknraja@yahoo.co.in
Address : Animal Genetic Resources Division, ICAR-National Bureau of Animal Genetic Resources, Karnal-132001, Haryana, India
Submitted Date : 13-01-2017
Accepted Date : 17-04-2017

Abstract

Rajapalayam, an important dog breed of southern India which are maintained for guarding of farm houses and protection from wild animals. The aim of the present investigation is to study the karyology of this important dog population for cytological profiling. Metaphase plates were prepared after culturing of lymphocytes isolated from heparinized blood collected from animals of both the sexes. Giemsa banding, centromeric index, arm ratio and relative length were estimated through standard protocol. The cytogenetic profile of Rajapalayam dog is different from that of Chinese Raccoon and Japanese Raccoon dogs which belongs to Canidae family with a basic chromosome number of 78 without B chromosome. The X chromosomes are longer in bitches than that of in dogs. Giemsa banding analysis revealed higher number of bands (272) distributed among the 38 chromosome in Indian dog breed as compared to that Chinese Raccoon dog. The results of the present study gave insight knowledge to the researchers, dog breeders and kennel clubs about the karyology of Indian dog breeds and to know about any chromosomal abnormalities which may leads to fertility, growth and phenotypic abnormality related problems in this dog breed.   

Keywords

G banding Karyotype Rajapalayam dog.

References

  1. Barch M J, Lawee H J and Arsham M S. (1991). Peripheral blood culture. In: The ACT cytogenetics Laboratory Manual, [Barch, M.J. (Ed.)] 2nd edition Newyork, Raven Press. pp. 17-30. 
  2. Ma¨kinen Auli, Majra-Terttu Kuokkanen and Maija Valtonen. (1986). A chromosome-banding study in the Finnish and the Japanese Raccoon dog. Hereditas 10: 97-105.
  3. Moorhead P S, Nowell P C, Mellman WJ , Battips D M and Hungerford D.A. (1960). Chromosome preparation of leukocytes cultured from human peripheral blood. Exp Cell Res 20: 613-616. 
  4. Pieñkowska A, Szczerbal I, Mäkinen A and Œwitoñski, M. (2002). G/Q-banded chromosome nomenclature of the Chinese Raccoon dog, Nyctereutes procyonoides procyonoides Gray. Hereditas 137: 75-78.
  5. Sumner A T, Evans H Tand Buckland R A. (1971). New technique for distinguishing between human chromosomes. Nature N B 232: 31-32. 
  6. Suryawanshi A R, Kanadkhedkar H L and Umrikar U D. (2004). Chromosome analysis of Domestic Dogs. Indian Vet J 81: 1071-1073.
  7. Switonski M, Rogalska-Niznik N, Szczerbal I and Baer M. (2003). Chromosome polymorphism and karyotype evolution of four canids: the dog, red fox, arctic fox and raccoon dog. Caryologia. 56 (4): 375-385.
  8. Topashka-Ancheva M, Gerasimova Ts, Dinchev V and Dimitrov K. (2009). Karyological data about the Bulgarian native dog breed “Karakachan Dog”. Biotechnol Biotec Eq 23: sup1, 136-139, DOI: 10.1080/13102818.2009.10818384.
  9. Wada M Y and Imai H T. (1991). On the Robertsonian polymorphism found in the Japanese Raccoon dog (Nyctereutes procyonoides viverrinus). Jpn J Genet 66: 1–11.
  10. Wada M Y, Suzuki T and Tsuchiya K. (1998). Re-examination of the chromosome homology between two subspecies of Japanese Raccoon dogs (Nyctereutes procyonoides albus and N.p. viverrinus). Caryologia 51 (1): 13-18.

Global Footprints