Chief EditorK.M.L. Pathak
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Online ISSN 0976-0555
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Molecular detection of Bovine Herpes Virus-1 (BoHV-1), Bovine Herpes Virus-4 (BoHV-4) and Bovine Viral Diarrhoea Virus (BVDV) in aborted ruminant fetuses from Kars province in Northeast Turkey
First Online 14-03-2016|
The purpose of this study was to identify the presence of Bovine Herpes Virus-1 (BoHV-1), Bovine Herpes Virus-4 (BoHV-4) and Bovine Viral Diarrhea Virus (BVDV) in the tissue of aborted ruminant fetuses using molecular methods. Therefore, internal organ samples (lung, liver and spleen, total n=48) were collected from ruminant abortions (10 lambs and 12 calves) in small-scale family operations in the province of Kars, Turkey. The presence of BoHV-1 and BoHV-4 nucleic acids was investigated with polymerase chain reaction (PCR) specific for the Glycoprotein C (gC) and Glycoprotein B (gB) gene respectively, and the presence of BVDV nucleic acid with reverse transcriptase polymerase chain reaction (RT-PCR). BoHV-1 and BoHV-4 nucleic acids could not be detected in any of the tissue samples. However, eight of the twelve aborted calves (66.66%) and thirteen material samples (52%) from twenty-five of the internal organs from these calves tested positive for BVDV RNA. Data obtained from this study and identification of the BVDV-specific antigen in aborted fetuses in the province of Kars proves that the BVDV infection is present in the region and plays an important role in cases of abortion observed in cattle. In addition, BoHV-1 and BoHV-4 do not seem to play a role in cases of ruminant abortion in sampled fotuses.
- Ackermann, M. and Engels, M. (2006). Pro and Contra IBR eradication. Vet. Microbiol., 113: 293-302.
- Albayrak, H., Okur Gumusova, S., Ozan, E. and Yazici, Z. (2012). Molecular detection of pestiviruses in aborted foetuses from provinces in northern Turkey. Trop. Anim. Health. Prod., 44: 677–680
- Belak, S. and Palfi, V. (1974). Characterization of a herpesvirus isolated from spontaneously degenerated bovine kidney cell culture. Acta. Vet. Acad. Sci. Hungar., 24: 249-253
- Bilge Dagalp, S., Güngör, E., Demir, A.B., Pýnar Muz, D., Yilmaz, V., Oguzoglu, T.C., Ataseven, V. and Alkan, F. (2010). The Investigation of The Presence of Bovine Herpesvirus type 4 ( BoHV-4) in cows with metritis in a dairy herd. Ankara Univ. Vet. Fak. Derg., 57: 87-91
- Bilge Dagalp, S., Alkan, F., Caliskan, E., Yildirim, Y., Oguzoglu, T.C., Can Sahna, K. and Burgu, I. (2012). The investigation of the herpesviruses (BoHV-1 and BoHV-4) on the occurrence of the reproductive disorders in dairy cattle herds, Turkey. Revue. Méd. Vét., 163: 206-211
- Bitgel, A. (1997). Detection of IBR, RSV and PI-3 antigens by IFAT in Turkey Pendik. Vet. Mikrobiyol. Derg., 28: 127-140
- Czaplicki, G. and Thiry, E. (1998). An association exists between bovine herpesvirus-4 seropositivity and abortion in cows. Prev. Vet. Med., 33:235–240
- Deim, Z., Szeredi, L. and Egyed, L. (2007). Detection of Bovine herpesvirus 4 DNA in aborted bovine fetuses. Can. J. Vet. Res., 71: 226–229
- Dubious, E., Russo, P., Prigent, P. and Thiery, R. (2008). Genetic characterization of ovine pestiviruses isolated in France, 1985 and 2006. Vet. Microbiol., 130: 69-79
- Grooms, D.L. (2004). Reproductive consequences of infection with bovine viral diarrhea virus. Vet. Clin. Nort. Am.: Food Anim. Pract., 20: 5–19
- Hasircioglu, S., Kale, M. and Acar, A. (2009). Investigation of pestiviruses infections in aborted sheep and goats in Burdur region, Kafkas. Univ. Vet. Fak. Derg., 15: 163–167
- Lamm, C.G., Broaddus, C.C. and Holyoak, G.R. (2009). Distribution of bovine viral diarrhea virus antigen in aborted fetal and neonatal goats by immunohistochemistry. Vet. Pathol., 46: 54–58
- Mahmoud, M.A. and Ahmed, S.A. (2009). Prevalence of bovine herpesvirus-1 in sheep and goats in Egypt. Global. Veterinaria., 3: 472-479
- Oguzoglu, T.C., Muz, D., Yilmaz, V., Alkan, F., Akca, Y. and Burgu, I. (2010). Molecular characterization of bovine virus diarrhea viruses species 2 (BVDV-2) from cattle in Turkey, Trop. Anim. Health. Prod., 42: 1175–1180
- Rasmussen, T.B., Reimann, I., Hoffmann, B., Depner, K., Uttenthal, A. and Beer, M. (2008). Direct recovery of infectious pestivirus from a full-length RT-PCR amplicon. J. Virol. Methods., 149:330-333
- Rocha, M.A., Barbosa, E.F., Guedes, R.M., Lage, A.P., Leite, R.C. and Gouveia, A.M. (1999). Detection of BHV-1 in a naturally infected bovine fetus by a nested PCR assay. Vet. Res. Commun., 23:133-41.
- Roizman, B., Desroisers, R.C., Fleckenstein, B., Lopez, C., Minson, A.C. and Studdert, M.J. (1992). The family herpesviridae: an update. Arch. Virol., 123: 425-449
- Saliki, J.T. and Dubovi, E.J. (2004). Laboratory diagnosis of bovine viral diarrhea virus infections. Vet. Clin. Nort. Am.: Food Anim. Pract., 20:69–83.
- Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989). Molecular cloning. In: Sambrook J, Fritsch EF, Maniatis T (Eds.) A Laboratory Manual. Cold Spring Harbor Laboratory Press, New York.
- Sasani, F., Vazirian, A., Javanbakht, J. and Aghamohammd Hassan, M. (2013). Detection of Infectious Bovine Rhinotracheitis in Natural Cases of Bovine Abortion by PCR and Histopathology Assays. American Journal of Clinical and Experimental Medicine 1:35-39 doi: 10.11648/j.ajcem.20130102.11
- Scott, J.C., Dutta, S.K. and Myrup, A.C. (1983). In vivo harboring of EHV-1 in leukocyte populations and subpopulations and their quantitation from experimentally infected ponies. Am. J. Vet. Res., 44: 1344–1348
- Seal, B.S. and Whetstone, C.A. (1994). Immedinate-early gene expression and gene mapping comparisons among isolates of bovine herpesvirus 1 and 5. Vet Microbiol 38: 369-84
- Sevik, M. and Avci, O. (2015). Molecular detection of Bovine Herpesvirus Type 1 from aborted small ruminant foetuses. Eurasian. J. Vet. Sci., 31: 57-59
- Takiuchi, E., Me´dici, K.C., Alfieri, A.F. and Alfieri, A.A. (2005). Bovine herpesvirus type 1 abortions detected by a semi- nested PCR in Brazilian cattle herds. Res. Vet. Sci., 79: 85–88
- Tan, M.T., Karaoglu, M.T., Erol, N., and Yildirim, Y. (2006). Serological and virological investigations of bovine viral diarrhoea virus (BVDV) infection in dairy cattle herds in Aydin province. Turk. J. Vet. Anim. Sci., 30: 299–304
- Wellemans, G., Van Opdenbosch, E. and Mammerickx, M. (1986). Experimental inoculation of bovine herpesvirus 4 (strain LVR 140) in pregnant and nonpregnant cows. Ann. Res. Vet., 17:89–94
- Wellenberg, G.J., Verstraten, E., Belak, S., Verschuren, F.A.M., Rijsewijk, R., Peshev, R. and Van Oirschot, J.T. (2001). Detection of bovine herpes virus 4 glycoprotein B and thymidine kinase DNA by PCR assays in bovine milk. J. Virol. Methods., 97: 101-112
- Willoughby, K.,Valdazo-Gonzalez, B., Laey, M., Gilray, M. and Nettleton, P.F. (2006). Development of a real time RT- PCR to detect and type ovine pestiviruses. J. Virol. Methods., 132: 187-194
- Van Engelenburg, F.A.C, Maes, R.K., Van Oirsschot, J.T. and Rijsewijk, F.A.M. (1993). Development of a rapid and sensitive polymerase chain reaction assay for detection of bovine herpesvirus type 1 in bovine semen. J. Clin. Microbiol., 31: 3129-35
- Vilcek, S., Herring, A.J., Herring, J.A., Nettleton, P.F., Lowings, J.P. and Paton, D.J. (1994). Pestiviruses isolated from pigs, cattle and sheep can be allocated into at least three genogroups using polymerase chain reaction and restriction endonuclease analysis. Arch. Virol. 136: 309-23
- Yavru, S., Avci, O. and Kale, M. (2014). Serologic and virologic investigation of BHV-1, BVDV and BHV-4 in cattle with metritis. Animal and Veterinary Sciences., 2(5):142-145 doi: 10.11648/j.avs.20140205.12
- Yazici, Z., Serdar, M.S., Okur Gumusova, S. and Albayrak, H. (2012). Molecular diagnosis and seroepidemiology of pestiviruses in sheep. Vet. Arhiv., 82: 35-45
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