Indian Journal of Animal Research

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Research Article

Indian Journal of Animal Research, volume 52 issue 1 (january 2018) : 111-115

Characterization of enterotoxin producing Clostridium perfringens isolated from foods of animal origin

Shahnaz Haque, Rajeev Kumar Sharma, Nagendra Nath Barman, Laishram Babita Devi, Luit Moni Barkalita, Iftikar Hussain
1Department of Microbiology, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati-781022, Assam
Cite article:- Haque Shahnaz, Sharma Kumar Rajeev, Barman Nath Nagendra, Devi Babita Laishram, Barkalita Moni Luit, Hussain Iftikar (2017). Characterization of enterotoxin producing Clostridium perfringens isolated from foods of animal origin. Indian Journal of Animal Research. 52(1): 111-115. doi: 10.18805/ijar.v0iOF.9129.

ABSTRACT

Screening of 105 samples comprising of milk, meat and their products for isolation of Clostridium perfringens revealed a total of 39 (37.14%) samples to be positive for C. perfringens, yielding an equal number of isolates. Among the total isolates, 11 (20.0%) belonged to milk and milk products, of which 10 were isolated from raw cow’s milk. The remaining isolate was from paneer sample. Similarly, 28 (56.0%) C. perfringens isolates were recovered from meat and meat products. Majority (37) of the isolates belonged to toxin type A while two isolates recovered each from raw beef and chicken meats were of toxin type C. Among the 39 isolates of C. perfringens, only 5(12.82%) isolates could reveal the presence of cpe (enterotoxin) gene. The protein profile (SDS-PAGE) of both the crude and partially purified enterotoxins prepared from cpe positive C. perfringens isolates (type A and C) revealed a very little difference. Both the partially purified enterotoxins of type A and C of C. perfringens displayed almost similar type of cytopathic effects (CPE) and death of cells in Vero cell line.

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