Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

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Indian Journal of Animal Research, volume 52 issue 6 (june 2018) : 875-881

Effects of dietary protein and lipid levels on growth, feed utilization and body composition in juvenile goldfish, Carassius auratus

S.S. Belsare, Hukam Singh Dhaker, A.S. Pawase, V.R. Joshi, S.A. Mohite, R.H. Rathod
1<p>College of Fisheries, Dr. B.S. Konkan Agriculture University, Ratnagiri 415 629, Maharashtra, India.</p>
Cite article:- Belsare S.S., Dhaker Singh Hukam, Pawase A.S., Joshi V.R., Mohite S.A., Rathod R.H. (2017). Effects of dietary protein and lipid levels on growth, feed utilization and body composition in juvenile goldfish, Carassius auratus . Indian Journal of Animal Research. 52(6): 875-881. doi: 10.18805/ijar.v0iOF.7816.

An 8-week feeding trial was conducted to determine the optimum dietary protein and lipid levels for growth, feed utilization and body composition of goldfish, Carassius auratus juveniles (1.66 ± 0.018 g). Six diets containing two protein levels (300 and 400 g/kg) and three lipid levels (40, 80 and 120 g/kg) were formulated. Fifteen fish were randomly allotted to 18 aquaria (0.6 × 0.3 × 0.45 m) and fed to apparent satiation. The results showed that weight gain and specific growth rate (SGR) increased in response to higher dietary proteins with highest weight gain and SGR recorded in diet containing 400 g/kg protein with 80 g/kg lipid. Both feed intake (FI) and protein efficiency ratio (PER) were dependent on dietary protein and lipid level (P<0.05). Lipid retention (LR) was negatively correlated while hepatosomatic index (HSI) and viscerosomatic index (VSI) was positively correlated with dietary lipid levels. Whole body protein content decreased (P<0.05) while, lipid content increased with increasing dietary lipid at each protein level. Second-order polynomial regression also revealed diet containing 400 g/kg protein level and 80 g/kg lipid level optimal for the growth and feed utilization of juvenile goldfish, C. auratus. 


  1. Ali, M.Z. and Jauncey, K. (2005). Approaches to optimizing dietary protein to energy ratio for African catfish, Clarius gariepinus (Burchell, 1822). Aquacult. Nutr. 11: 95-101.

  2. Aminikhoei, Z., Choi, J. and Lee, S.M. (2015). Optimal dietary protein and lipid levels for growth of juvenile israeli carp Cyprinus carpio. Fish. Aquat. Sci. 18: 265-271.

  3. AOAC, (2006). Official Methods of Analysis of the Association of Official Analytical Chemists, 18th edn. Association of Official Analytical Chemist, Washington, D.C.

  4. Bandyopadhyay, P., Swain, S.K. and Mishra, S. (2005). Growth and dietary utilisation in goldfish (Carassius auratus Linn.) fed diets formulated with various local agro-produces. Bioresour. Technol. 96: 731-740.

  5. Catacutan, M.R. and Coloso, R.M. (1995). Effect of dietary protein to energy ratios on growth, survival and body composition of juvenile Asian seabass, Lates calcarifer. Aquaculture. 131: 125-133.

  6. Cho, C. Y. and Bureau, D. P. (1995). Determination of the energy requirements of fish with particular reference to salmonids. J. Appl. Ichthyol. 11: 141–163.

  7. Chou, B.-S. and Shiau, S.-Y (1996). Optimal dietary lipid level for growth of juvenile hybrid tilapia Oreochromis niloticus × Oreochromis aureus. Aquaculture. 143: 185-195.

  8. De Silva, S.S. and Anderson, T.A. (1995). Fish Nutrition in Aquaculture. Chapman & Hall, London, UK. pp. 319.

  9. Du, Z.Y., Liu, Y.J., Tian, L.X., Wang, J.T., Wang, Y. and Liang, G.Y. (2005). Effect of dietary lipid level on growth, feed utilization and body composition by juvenile grass carp (Ctenopharyngodon idella). Aquacult. Nutr. 11: 139-146.

  10. Earle, K.E. (1995). The nutritional requirements of ornamental fish. Vet. Q. 17 (Suppl. 1): S53–S55.

  11. Espinós, F.J., Tomás, A., Pérez, L.M., Balasch, S. and Jover, M. (2003). Growth of dentex fingerlings (Dentex dentex) fed diets containing different levels of protein and lipid. Aquaculture. 218: 479-490.

  12. Fiogbé, E.D. and Kestemont, P. (1995). An assessment of the Protein and amino acid requirements in Goldfish (Carassius auratus) larvae. J. Appl. Ichthyol. 11: 282-289.

  13. Gangadhara, B., Nandesha, M.C., Varghese, T.J. and Keshavanath, P. (1997). Effect of varying protein and lipid levels on the growth of Rohu, Labeo rohita. Asian Fish. Sci. 10: 139-147.

  14. Gao, W., Liu, Y.-J., Tian, L.-X., Mai, K.-S., Liang, G.-Y., Yang, H.-J., Huai, M.-Y. and Luo, W.-J. (2011). Protein-sparing capability of dietary lipid in herbivorous and omnivorous freshwater finfish: a comparative case study on grass carp (Ctenopharyngodon idella) and tilapia (Oreochromis niloticus x O. aureus). Aquacult. Nutr. 17: 2-12.

  15. Jantrarotai, W., Sitasit, P., Jantrarotai, P., Viputhanumas, T. and Srabua, P. (1998). Protein and energy levels for maximum growth, diet utilization, yield of edible flesh and protein sparing of hybrid Clarias catfish (Clarias macrocephalus x Clarias gariepinus). J. World. Aquac. Soc. 29: 281-289.

  16. Kaushik, S.J.and Medale, F. (1994) Energy requirement, utilization and dietary supply to salmonids. Aquaculture. 121: 81-97.

  17. Kim, L.O. and Lee, S.-M. (2005). Effect of dietary protein and lipid levels on growth and body composition of bagrid catfish, Pseudobagrus fulvidraco. Aquaculture. 243: 323-329.

  18. Lee, S.-M. and Kim, K.-D. (2005). Effect of various levels of lipid exchanged with dextrin at different protein level in diet on growth and body composition of juvenile flounder Paralichthys olivaceus. Aquac. Nutr.11: 435–442.

  19. Lee, J.S. and Newman, M.E. (1997). Aquaculture Business, 2nd edn. Interstate Publishers, IL, USA. pp.393-432.

  20. Liu, X.Y., Wang, Y. and Ji, W.X. (2011). Growth, feed utilization and body composition of Asian catfish (Pangasius hypophthalmus) fed at different protein and lipid levels. Aquacult. Nutr. 17: 578-584.

  21. Lochmann, R. and Phillips, H. (1994). Dietary protein requirement of juvenile Golden Shiners (Notemigonus crysoleucas) and Goldfish (Carassius auratus) in aquaria. Aquaculture, 128, 277–285.

  22. Mohanta, K.N. and Subramanian, S. (2002). Effect of diets with protein from different sources on the growth of goldfish, Carassius auratus. Israeli J. Aquacult-Bamidgeh. 54: 134-140.

  23. Mohanta, K.N., Mohanty, S.N., Jena, J. and Sahu, N.P. (2009). A dietary energy level of 14.6 MJ kg-1 and protein-to-energy ratio of 20.2g MJ-1 results in best growth performance and nutrient accretion in silver barb Puntius gonionotus fingerlings. Aquacult. Nutr. 15: 627-637.

  24. National Research Council, (2011). Nutrient Requirements of Fish. The National Academy Press, Washington, DC, USA.

  25. Page, J.W. and Andrews, J.W. (1973). Interaction of dietary levels of protein and energy on channel catfish (Ictalurus punctatus). J. Nutr. 103: 1339-1346.

  26. Salhi, M., Bessonart, M., Chediak, G., Bellagamba, M. and Carnevia, D. (2004). Growth, feed utilization and body composition of black catfish, Rhamdia quelen fry fed diets containing different protein and energy levels. Aquaculture, 231: 435-444.

  27. Schuchardt, D., Vergara, J.M., Fernández-Palacios, H., Kalinowski, C.T., Hernández-Cruz, C.M., Izquierdo, M.S. and Robaina, L. (2008). Effects of different dietary protein and lipid levels on growth, feed utilization and body composition of red porgy (Pagrus pagrus) fingerlings. Aquacult. Nutr. 14: 1-9.

  28. Smartt, J. (2001). History and development of the Goldfish. In: Goldfish Varieties and Genetics: A Handbook for Breeders (Smartt, J. eds). Fishing News Books, Blackwell Science Ltd., Oxford. pp. 11-25.

  29. Snedecor, G.W. and Cochran, W.G. (1967). Statistical Methods, 6th ed. Oxford and IBH Publishing Co., New Delhi, pp. 593.

  30. Wang, Y.Y., Ma, G.J., Shi, Y., Liu, D.S., Guo, J.X., Yang, Y.H. and Chen, C.D. (2013). Effects of dietary protein and lipid levels on growth, feed utilization and body composition in Pseudobagrus ussuriensis fingerlings. Aquacult. Nutr. 19: 390-398. 

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