Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

  • Print ISSN 0367-6722

  • Online ISSN 0976-0555

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Indian Journal of Animal Research, volume 51 issue 6 (december 2017) : 1057-1061

Some immunological studies on a new vaccine derived from bacteriophage lysate of Pasteurella multocida in mice

Yanglem Pushpa, Sabia Qureshi, Hari Mohan Saxena
1<p>Department of Veterinary Microbiology, College of Veterinary Science,&nbsp;GADVASU, Ludhiana-141 004, Punjab, India.</p>
Cite article:- Pushpa Yanglem, Qureshi Sabia, Saxena Mohan Hari (2016). Some immunological studies on a new vaccine derived frombacteriophage lysate of Pasteurella multocida in mice . Indian Journal of Animal Research. 51(6): 1057-1061. doi: 10.18805/ijar.v0iOF.7000.

The study was undertaken to evaluate the humoral immune response in mice induced by a novel phage lysate vaccine against Hemorrhagic Septicemia developed from Pasteurella multocida grown under iron-restricted conditions and lysed by a bacteriophage. Two groups of mice were immunized with lysate vaccine (LV) and conventional killed HS vaccine (CV) and blood was collected at various days post – immunization (DPI). The serum protein concentration in lysate vaccinated mice (4.675±0.223) was significantly higher (p<0.05) than the conventional vaccinated mice (4.100±0.282) at 150 DPI. The serum globulin levels at 90 DPI and 180 DPI in LV mice (1.330±0.071 and 0.650±0.100) were significantly (p<0.01) higher than the CV mice (0.850±0.084 and 0.366±0.098). The serum IgG levels at 150 DPI and 180 DPI in LV (0.564±0.188 and 0.485±0121) mice were significantly higher (p<0.01) than the CV mice (0.178±0.039 and 0.121±0.026). Although our results in mice are promising, further studies involving bovines are needed.

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