Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

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Indian Journal of Animal Research, volume 52 issue 4 (april 2018) : 569-573

In vitro nutritional evaluation and methane production of deoiled ajwain meal as a potential ruminant feed ingredient

Mayank Gautam, J.P. Sehgal, Madhu Mohini, Ritika Gupta, Tarun Kumar Varun, Srobana Sarkar
1<p>Animal Nutrition Division,&nbsp;ICAR-National Dairy Research Institute, Karnal-132 001, Haryana, India.</p>
Cite article:- Gautam Mayank, Sehgal J.P., Mohini Madhu, Gupta Ritika, Varun Kumar Tarun, Sarkar Srobana (2016). In vitro nutritional evaluation and methane production of deoiledajwain meal as a potential ruminant feed ingredient . Indian Journal of Animal Research. 52(4): 569-573. doi: 10.18805/ijar.v0i0f.3788.

The present study was undertaken to evaluate deoiled ajwain (Trachyspermum ammi) meal (DOAM) for its in vitro nutritional evaluation and methane mitigation potential. For this purpose, a concentrate mixture based on maize and soybean meal as a major feed ingredient was formulated and designated as control ‘C’. The concentrate mixture was reformulated using 5, 10, 15 and 20 parts (kg/100kg) of DOAM for experimental groups i.e. T1, T2, T3 and T4, respectively and made iso-nitrogenous by addition of urea. The values of DM, CP, EE, CF, TA, NFE, NDICP and ADICP (%) in DOAM were 88.41, 14.57, 2.65, 16.35, 12.75, 53.68, 51.86 and 17.35, respectively. Dry matter degradability and digestibility of T1 and T2 were comparable with those of control whereas significant (P<0.05) depression was observed when 15% (T3) and 20% (T4) of DOAM was incorporated. Addition of 10% DOAM in the concentrate mixture caused significant (P<0.05) decrease of methane production from 46.65 mg/g DM (control) to 39.70 ml/gm DM. It can be concluded from the present study that addition of 10% deoiled ajwain meal (DOAM) in the concentrate in concentrate mixture resulted in lower methane production and has no adverse effect on DM, OM and CP digestibility.


  1. Agarwal, N., Shekhar, C., Kumar, R., Chaudhary, L.C. and Kamra, D.N. (2009). Effect of peppermint (Mentha piperita) oil on in vitro methanogenesis and fermentation of feed with buffalo rumen liquor. Anim. Feed Sci. Technol., 148: 321-327.

  2. Alan, L. and Miller, N.D. (1996). Antioxidant flavonoids, structure, function and chemical usage. Alt. Med. Rev., 1: 103-111.

  3. AOAC. (2005). Official Methods of Analysis. 18th Ed. Association of Official Analytical Chemists, Maryland, USA.

  4. Calsamiglia, S., Busquet, M., Cardozo, P.W., Castillejos, L. and Ferret, A. (2007). Invited review: Essential oils as modifiers of rumen microbial fermentation, J. Dairy Sci., 90: 2580–2595.

  5. Eckard, R.J., Grainger, C. and De Klein, C.A.M. (2010). Options for the abatement of methane and nitrous oxide from ruminant production: A review, Livest. Sci., 130: 47–56.

  6. Hu, W.L., Liu, J.X., Ye, J.A., Wu, Y.M. and Guo, Y.Q. (2005). Effect of tea saponin on rumen fermentation in vitro. Anim. Feed Sci. Technol., 120: 333–339.

  7. IPCC. (2007). Climate Change 2007: Impacts, Adaptation and Vulnerability. Intergovernmental Panel on Climate Change, Brussels. 

  8. Javed, S., Shahid, A.A., Haider, M.S., Umeera, A., Ahmad, R. and Mushtaq, S. (2012). Nutritional, phytochemical potential and pharmacological evaluation of Ngella sativa (Kalonji) and Trachyspermum ammi (Ajwain). J. Med. Plants Res., 6: 768-775.

  9. Krishnamoorthy, U., Sniffen, C.J., Stern, M.D. and Van Soest, P.J. (1983). Evaluation of a mathematical model of rumen digestion and an in vitro simulation of rumen proteolysis to estimate the rumen-undegraded nitrogen content of feedstuffs. Br. J. Nutr., 50: 555-568.

  10. Licitra, G.T., Harnandez, M. and Van Soest, P.J. (1996). Standardizations of procedures for nitrogen fractionation of ruminant feeds. Anim. Feed Sci. Technol., 57: 347-358. 

  11. Menke, K.H. and Steingass, H. (1988). Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid. Anim. Res. Dev., 28: 7-55.

  12. Niwas, R., Singh, D.P., Paswan, V.K., Albial, A.M. and Kumar, S. (2013). Comparative impact of formulated herbal nutraceuticals on performance of crossbred calves. The Bioscan, 8: 519-521.

  13. Patra, A.K., Kamra, D.N. and Agarwal, N. (2006). Effect of plant extract on in vitro methanogenesis, enzyme activities and fermentation of feed in rumen liquor of buffalo. Anim. Feed Sci. Technol., 128: 276–291.

  14. Patra, A.K., Kamra, D.N., Agarwal, N. and Chatterjee, P.N. (2009). Effects of Terminalia belerica and Allium sativum as feed additives on rumen fermentation, enzyme activities and microbial populations in Murrah buffaloes. Indian J. Anim. Nutr., 24: 251-255.

  15. Pawar, M.M., Kamra, D.N., Agarwal, N. and Chaudhary, L.C. (2014). Effects of essential oils on in vitro methanogenesis and fermentation of feed with buffalo rumen liquor. Agric. Res., 3: 67- 74.

  16. Pruthi, J.S. (1992). Spices and Condiments. 4th ed., National Book Trust, New Delhi, India.

  17. Sachan, J., Kumar, R., Vaswani, S., Kumar, V. and Roy, D. (2014). Effect of addition of herbs on in vitro rumen fermentation and digestibility of feed. Indian J. Anim. Res., 48: 88-90.

  18. Snedecor, G.W. and Cochran, W.C. (1994). Statistical Methods, 8th Ed. Oxford and IBH Publishing Co., New Delhi, India.

  19. Sniffen, C.J., O’Connor, J.D., Van Soest, P.J., Fox, D.G. and Russel, J.B. (1992). A net carbohydrate and protein system for evaluating cattle diets II Carbohydrate and protein availability. J. Anim. Sci., 70: 3562-3577. 

  20. SPSS. (2007). Statistical Packages for Social Sciences. Version 16, SPSS Inc., Linois, USA.

  21. Tavendale, M.H., Meagher, L.P., Pacheco, D., Walker, N., Attwood, G.T. and Sivakumaran, S. (2005). Methane production from in vitro rumen incubations with Lotus pedunculatus and Medicago sativa, and effects of extractable condensed tannin fractions on methanogenesis. Anim. Feed Sci. Technol., 123–124: 403–419.

  22. Tilley, J.M.A. and Terry, R.A. (1963). A two stage technique for in-vitro digestion of forage crops. J. Br. Grassland Soc., 18: 104-111.

  23. Treas, G.E. and Evans, W.C. (2002). Pharmacognosy, International 15th edition, Saunder Edinburgh, New York, pp 258.

  24. Tsimidou, M. and Boskou, D. (1994). Antioxidant activity of essential oils from the plants of the Lamiaceae family, In Spices, Herbs and Edible Fungi, pp. 273-284.

  25. Van Soest, P.J., Robertson, J.B. and Lewis, B.A. (1991). Methods for dietary fibre, neutral detergent fibre and non-starch polysaccharides in relation to animal nutrition. J. Dairy Sci., 74: 3583-3597.

  26. Yadav, R.P. and Singh, D.P. (2008). The effect of herbal drug on growth performance of crossbred calves. Asian J. Animal Sci., 3: 210-214. 

  27. Yatoo, M.A., Chaudhary, L.C., Agarwal, N. and Kamra, D.N. (2014). Effect of a blend of essential oils on in vitro methanogenesis and feed fermentation with buffalo rumen liquor. Anim. Feed Sci. Technol., 14: 371-377.

     

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