Loading...

Histology and immunohistochemistry of the palatine tonsil in goats

DOI: DOI:10.18805/ijar.v0iOF.7657    | Article Id: B-3248 | Page : 508-512
Citation :- Histology and immunohistochemistry of the palatine tonsil in goats .Indian Journal of Animal Research.2018.(52):508-512

V.R. Indu, K.M. Lucy, N. Ashok, S. Maya and P.M. Priya

indu@kvasu.ac.in
Address :

Department of Veterinary Anatomy, College of Veterinary and Animal Sciences, Mannuthy- 680651, Kerala, India.

Submitted Date : 25-05-2016
Accepted Date : 14-02-2017

Abstract

Histological studies were conducted on the palatine tonsil of six male crossbred goats of six months of age. The tonsils were lined by a stratified squamous non-keratinized surface epithelium which continued into the crypts forming the non-reticular epithelium.  At some areas in the crypts, the non-reticular epithelium associated with lymphoid follicles showed a great reduction in height with only one to two intact cell layers and were called reticular epithelium or lymphoepithelium. Propria-submucosa of the palatine tonsil was characterized by dense irregular connective, lymphoid, glandular, adipose and muscular tissues. Lymphoid tissue constituted majority of the palatine tonsil and was organized into primary and secondary lymphoid nodules and dense diffuse lymphatic tissue. Average diameter of lymphoid nodules was 684.17±6.88µm while the lymphocyte count in the nodules was 28826.54±236.25.  The average number of lymphatic nodules counted per field under low power magnification of microscope was 2.67±0.42 and the internodular distance was 34.67±1.41. Glandular tissue was present in the deeper areas of propria-submucosa. A well developed connective tissue capsule separated the lymphoid and glandular tissues of the palatine tonsil. In the immunohistochemical staining technique strong positive reaction for cytoplasmic IgG bearing B- lymphocytes was noticed within the germinal centre of lymphoid nodules, towards the base of the FAE and some cells even infiltrated the crypt epithelium.  In the mantle zone and internodular area, reaction was very mild indicating that T- lymphocytes predominated in these areas.  It was concluded that the palatine tonsils were histologically mature as a local defence mechanism against the harmful substances to be encountered from the environment after birth. 
 

Keywords

Goats Histology Immunohistochemistry Palatine Tonsil.

References

  1. Anderson, J.C. (1974). The response of the tonsil and associated lymph nodes of gnotobiotic piglets to the presence of bacterial antigen in the oral cavity. J. Anat. 117: 191-198.
  2. Bancroft, J.D. and Gamble, M. (2003). Theory and Practice of Histological Techniques. (5th Ed.). Churchill Livingstone, New York, 796p. 
  3. Breugelmans, S., Spiegelaere, W.D., Casteleyn, C., Simoens, P. and Van den Broeck, W. (2011). Differences between the ovine tonsils based on an immunohistochemical quantification of the lymphocyte subpopulations. Comp. Immunol., Microbiol. Infect. Dis. 34: 217–225.
  4. Baykan, M., Celik, I., Gezici, H., Donmez, H., Eken, H., Sur, E. and Ozkan, Y. (2001). A light microscopic study on the uptake and transportation route of carbon particles in the canine palatine tonsil. Revue Méd. Vét. 152: 709-715.
  5. Belz, G.T. and Heath, T.J. (1995). Intercellular and lymphatic pathways of the canine palatine tonsils. J. Anat. 187: 93-105.
  6. Casteleyn, C., Broeck, V.W. and Simoens, P. (2007). Histological characteristics and stereological volume assessment of the ovine tonsils. Vet. Immunol. Immunopathol.120: 124–135.
  7. Cocquyt, G., Baten, T., Simoens, P. and Broeck, W.V.D. (2005). Anatomical localisation and histology of the ovine tonsils. Vet. Immunol. Immunopathol. 107: 79–86.
  8. Kelly, D.E., Wood, R.L. and Enders, A.C. (1983). Lymphatic organs. In: Bailey’s Textbook of Microscopic Anatomy. (18thEd.). Williams and Wilkins, London, pp. 438-471.
  9. Kumar, P., Kumar, P. and Kumar, S. (2006). Light and scanning electron microscopic studies on the nasopharyngeal tonsil of the goat. Indian J. Anim. Sci. 76: 452–455.
  10. Kumar, P., Mahesh, R., Singh, G. and Nagpal, S.K. (2008). Light microscopic studies on the palatine tonsil of sheep. Haryana Vet. 47: 15-18.
  11. Kumar, P. and Timoney, J.F. (2005a). Histology, immunohistochemistry and ultrastructure of the equine palatine tonsil. Anat. Histol. Embryol. 34: 192–198. 
  12. Luna, L.G. (1968). Manual of Histological Staining Methods of the Armed Forces Institute of Pathology. (3rd Ed.). Mc Graw-Hill Book Company, New York, 258p.
  13. Perry, M. and Whyte, A. (1998). Immunology of the tonsils. Immunol. Today. 19: 414–421.
  14. Raju, N.K.B, Geetha, R, Sabiba, H.B and Usha Kumary, S.(2012) Histochemical studies on the palatine tonsil of sheep (Ovis aries). IJAVMS.6:392-397.
  15. Ramos, J.A., Ramis, A.J., Marco, A., Domingo, M., Rabanal, R. and Ferrer, L. (1992). Histochemical and immunohistochemical study of the mucosal lymphoid system in swine. Am. J. Vet. Res. 53: 1418-1426.
  16. Salles, M.W.S. and Middleton, D.M. (2000). Lymphocyte subsets in porcine tonsillar crypt epithelium. Vet. Immunol. Immunopathol. 77: 133-144.
  17. Singh, U.B. and Sulochana, S. (1996). Handbook of Histological and Histochemical Techniques. Premier Publishing House, Hyderabad, 111p.
  18. Sternberger, L.A. (1986). Immunocytochemistry. (3rd Ed.) John Wiley and Sons, New York. 257p.
  19. Tenorio, E.M.L. and Pabst, R. (2006). MALT structure and function in farm animals. Vet. Res. 37: 257–280.
  20. Velinova, M., Thielen, C., Mélot, F., Eicher, S., Heinen, E., Antoine, N. and Donga, J. (2001). New histochemical and ultrastructural observations on normal palatine tonsils. Vet. Rec. 149: 61. 
  21. Williams, D.M. and Rowland, A.C. (1972). The palatine tonsils of the pig - an afferent route to the lymphoid tissue. J. Anat. 113: 131-137.

Global Footprints