Sequence characterization and polymorphism detection in AQP7 gene of Murrah buffalo

DOI: 10.18805/ijar.v0iOF.4549    | Article Id: B-3237 | Page : 378-382
Citation :- Sequence characterization and polymorphism detectionin AQP7 gene of Murrah buffalo .Indian Journal Of Animal Research.2018.(52):378-382

Ragini Kumari, K.P. Ramesha, Rakesh Kumar, P. Divya, Anjali Kumari, Beena Sinha and D.S. Gonge

raginindri@gmail.com
Address :

Genetics Laboratory, Dairy Production Section, Southern Regional Station of ICAR- National Dairy Research Institute, Adugodi, Bengaluru-560 030, Karnataka, India.
 

Submitted Date : 10-05-2016
Accepted Date : 12-10-2016

Abstract

Aquaporin 7 (AQP7) gene is a member of aqua-glyceroporins which transports glycerol and water to spermatids. The present study aimed to investigate the polymorphisms within exons 2, 3, 4, 5 and their flanking intronic regions in AQP7 gene of Murrah bulls. Genomic DNA was extracted from 69 Murrah bulls blood samples and was subjected to polymerase chain reaction- single strand conformation polymorphism (PCR-SSCP) analysis. PCR-SSCP analysis revealed a total of eight different variants for amplicons of exons 4 and 5. Amplicon of exon 4 revealed three different patterns viz. E4P1, E4P2 and E4P3 with the frequency of 0.30, 0.22 and 0.48, respectively. Analysis of exon 5 revealed five unique SSCP patterns viz. E5P1, E5P2, E5P3, E5P4 and E5P5, with the frequency of 0.10, 0.37, 0.20, 0.20 and 0.13, respectively. Sequence analysis showed 16 single nucleotide polymorphisms, 7 of which were observed in coding region. Amplicons of exons 2 and 3 showed monomorphic patterns. However, compared to the reference sequence of taurine cattle one transition (C6878T) in exon 3 and 4 transitions (G2099A, C2116T, A2117G, G6848C) in intron 2 were observed for all the bulls under study. The genetic variability identified in the AQP7 gene may serve as potential genetic marker(s) for semen quality traits in buffalo.

Keywords

AQP7 gene Murrah bulls PCR-SSCP Polymorphism.

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