Sequence characterization and polymorphism detection in AQP7 gene of Murrah buffalo

DOI: 10.18805/ijar.v0iOF.4549    | Article Id: B-3237 | Page : 378-382
Citation :- Sequence characterization and polymorphism detectionin AQP7 gene of Murrah buffalo .Indian Journal Of Animal Research.2018.(52):378-382

Ragini Kumari, K.P. Ramesha, Rakesh Kumar, P. Divya, Anjali Kumari, Beena Sinha and D.S. Gonge
Address :

Genetics Laboratory, Dairy Production Section, Southern Regional Station of ICAR- National Dairy Research Institute, Adugodi, Bengaluru-560 030, Karnataka, India.

Submitted Date : 10-05-2016
Accepted Date : 12-10-2016


Aquaporin 7 (AQP7) gene is a member of aqua-glyceroporins which transports glycerol and water to spermatids. The present study aimed to investigate the polymorphisms within exons 2, 3, 4, 5 and their flanking intronic regions in AQP7 gene of Murrah bulls. Genomic DNA was extracted from 69 Murrah bulls blood samples and was subjected to polymerase chain reaction- single strand conformation polymorphism (PCR-SSCP) analysis. PCR-SSCP analysis revealed a total of eight different variants for amplicons of exons 4 and 5. Amplicon of exon 4 revealed three different patterns viz. E4P1, E4P2 and E4P3 with the frequency of 0.30, 0.22 and 0.48, respectively. Analysis of exon 5 revealed five unique SSCP patterns viz. E5P1, E5P2, E5P3, E5P4 and E5P5, with the frequency of 0.10, 0.37, 0.20, 0.20 and 0.13, respectively. Sequence analysis showed 16 single nucleotide polymorphisms, 7 of which were observed in coding region. Amplicons of exons 2 and 3 showed monomorphic patterns. However, compared to the reference sequence of taurine cattle one transition (C6878T) in exon 3 and 4 transitions (G2099A, C2116T, A2117G, G6848C) in intron 2 were observed for all the bulls under study. The genetic variability identified in the AQP7 gene may serve as potential genetic marker(s) for semen quality traits in buffalo.


AQP7 gene Murrah bulls PCR-SSCP Polymorphism.


  1. Agre, P. (2006). The aquaporin water channels. Proc. Am Thorac Soc., 3: 5-13
  2. Agre, P., King, L. S., Yasui, M., Guggino, W. B., Oltersen, O. P., Fujiyoshi, Y., Engel, A. and Nielsen, S. (2002). Aquaporin water channels from atomic structure to clinical medicine. J. Physiol., 542: 3-16.
  3. Beitz, E., Kumagami, H., Krippeit-Drews, P., Ruppersberg, J. P. and Schultz, J. E. (1999). Expression pattern of aquaporin water channels in the inner ear of the rat. The molecular basis for a water regulation system in the endolymphatic sac. Hear Res., 132: 76–84.
  4. Borgnia, M., Nielsen, S., Engel, A. and Agne, P. (1999). Cellular and molecular biology of the aquaporin water channels. Annu. Rev. Biochem., 68: 425-458.
  5. Calamita, G., Mazzone, A., Bizzoca, A. and Svelto, M. (2001). Possible involvement of aquaporin 7 and 8 in rat testis development and spermatogenesis. Biochem. Biophys. Res. Commun., 288: 619-625.
  6. Frigeri, A., Nicchia, G.P., Verbavatz, J.M., Valenti, G. and Svelto, M. (1998). Expression of aquaporin 4 in fast twitch fibres of mammalian skeletal muscle. J. Clin. Invest., 102: 695-703.
  7. Ishibashi, K., Kuwahara, M., Gu, Y., Kageyama, Y., Tohsaka, A., Suzuki, F., Marumo, F. and Sasaki, S. (1997). Cloning and functional expression of a new water channel abundantly expressed in the testis permeable to water, glycerol, and urea. J. Biol. Chem., 272: 20782-20786.
  8. Ishibashi, K., Morinaga, T., Kuwahare, M., Sasaki, S., Imai, M., (2002). Cloning and identification of a new member of water channel (AQP10) as an aquaglyceroporin. Biochim. Biophys. Acta., 1576: 335-340.
  9. Kumar, S., Deb, R., Singh, U., Ganguly, I., Mal, D.K., Singh, R., Sharma, S., Singh, G., Singh, R., Kumar, M. and Sharma, A. (2014). SNPs at exonic region of aquaporin 7 (AQP 7) gene may effects semen quality parameters among crossbred bulls. J. Genet., 93:108-112.
  10. Li, X., Yu, H. and Koide, S.S. (1994). The water channel gene in human uterus. Biochem. Mol. Biol. Int., 32: 371-377.
  11. Ma, T.H., Liu, J.F., Zhao, R.F., Jiang, H., Dai, L.S., Zhao, Y.M., Zhao, Z.H. and Zhang, J.B. (2011). Association analysis of aquaporin 7 (AQP7) gene variants with semen quality fertility in bulls. Turk. J. Vet. Anim. Sci., 35: 63-66.
  12. Miller, S.A, Dykes, D. and Polesky, H.F. (1988). A sample salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Res.,16:1215.
  13. Page, E., Winterfield, J., Goings, G., Bastawrous, A. and Upshaw-Earley, J. (1998). Water channel proteins in rat cardiac myocytecaveolae: osmolarity-dependent reversible internalization. Am J. Physiol. Heart Circ Physiol., 274:1988-    2000.
  14. Rui-feng, Z., Ji-feng. and Zhi-hui, Z. (2009). Single Nucleotide Polymorphism of exon 2 of aquaporin 7 gene and its association with semen quality in cow. China Herbivores.,4.
  15. Sambrook, J. and Russell, D. W. (2001). Molecular cloning. A laboratory manual. Vol 1: Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, pp: 1.32-1.34 and 8.14.
  16. Suzuki-Toyota, F., Ishibashi, K. and Yuasa, S. (1999). Immuno histochemical localization of a water channel, aquaporin 7 (AQP7), in the rat testis. Cell Tissue Res., 295: 279-285.
  17. Zardoya, R. (2005). Phylogeny and evolution of the major intrinsic protein family. Biol. Cell., 97: 397-414.

Global Footprints