Indian Journal of Animal Research

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Research Article

Indian Journal of Animal Research, volume 52 issue 3 (march 2018) : 353-357

Effect of growth factor on in-vitro maturation of porcine oocyte

N. Mahanta, D. Bhuyan, Suresh Kumar, R.K. Biswas, D.J. Dutta, B.C. Deka, A. Das, R.K. Dewry, G Hazarika
1Department of Animal Reproduction, Gynaecology & Obstetrics, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati 781 022, Assam, India
Cite article:- Mahanta N., Bhuyan D., Kumar Suresh, Biswas R.K., Dutta D.J., Deka B.C., Das A., Dewry R.K., Hazarika G (2018). Effect of growth factor on in-vitro maturation of porcine oocyte. Indian Journal of Animal Research. 52(3): 353-357. doi: 10.18805/ijar.B-3202.

ABSTRACT

The present study was aimed to evaluate the beneficial effect of different growth factors on in-vitro maturation of porcine oocytes. Ovaries were collected from a local abattoir immediately after slaughter of the animals and transported to the laboratory. A total of 618 type A and type B oocytes were cultured in TCM-199 containing additives with PMSG and  hCG for the first 22 hrs and without hormones for subsequent 22 hrs of incubation at 39o C under 5 per cent CO2 level and 90-95 per cent humidity. The effects of supplementation of different growth factors viz., EGF, IGF-I and EGF + IGF-I in the medium were studied. The rate of oocytes with cumulus cells expansion was significantly higher (P<0.01) when  growth factors were added as compared to control but it did not differ significantly between growth factors. The rate of nuclear maturation of oocytes was significantly higher (P<0.01) as compare to control for EGF and EGF + IGF-I but not for IGF-I. There was no significant difference in the rate of oocytes with nuclear maturation between the growth factors studied. It can be concluded from the present study that addition of EGF, IGF-I or EGF + IGF and additives along with hormones (PMSG and hCG for first 20-22 hrs) in TCM-199 Medium gives optimum in-vitro maturation rates in porcine oocytes.

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