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Research Article

volume 52 issue 3 (march 2018) : 353-357,   Doi: 10.18805/ijar.B-3202

Effect of growth factor on in-vitro maturation of porcine oocyte

N
N. Mahanta
D
D. Bhuyan
S
Suresh Kumar
R
R.K. Biswas
D
D.J. Dutta
B
B.C. Deka
A
A. Das
R
R.K. Dewry
G
G Hazarika
1Department of Animal Reproduction, Gynaecology & Obstetrics, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati 781 022, Assam, India
Cite article:- Mahanta N., Bhuyan D., Kumar Suresh, Biswas R.K., Dutta D.J., Deka B.C., Das A., Dewry R.K., Hazarika G (2018). Effect of growth factor on in-vitro maturation of porcine oocyte. Indian Journal of Animal Research. 52(3): 353-357. doi: 10.18805/ijar.B-3202.

ABSTRACT

The present study was aimed to evaluate the beneficial effect of different growth factors on in-vitro maturation of porcine oocytes. Ovaries were collected from a local abattoir immediately after slaughter of the animals and transported to the laboratory. A total of 618 type A and type B oocytes were cultured in TCM-199 containing additives with PMSG and  hCG for the first 22 hrs and without hormones for subsequent 22 hrs of incubation at 39o C under 5 per cent CO2 level and 90-95 per cent humidity. The effects of supplementation of different growth factors viz., EGF, IGF-I and EGF + IGF-I in the medium were studied. The rate of oocytes with cumulus cells expansion was significantly higher (P<0.01) when  growth factors were added as compared to control but it did not differ significantly between growth factors. The rate of nuclear maturation of oocytes was significantly higher (P<0.01) as compare to control for EGF and EGF + IGF-I but not for IGF-I. There was no significant difference in the rate of oocytes with nuclear maturation between the growth factors studied. It can be concluded from the present study that addition of EGF, IGF-I or EGF + IGF and additives along with hormones (PMSG and hCG for first 20-22 hrs) in TCM-199 Medium gives optimum in-vitro maturation rates in porcine oocytes.

REFERENCES

  1. Abeydeera, L. R., Wang, W. H., Cantley, T.C., Rieke. A., Murphy. C. N. and Prather. R. S. (2000). Development and viability of pig oocytes matured in a protein-free medium containing epidermal growth factor. Theriogenology., 54: 787–797.
  2. Bastan, A., Polat. B., Acar. D. B., Korkmaz. O. and Colak. A. (2010). Determination of optimal dose of EGF for bovine oocyte maturation and subsequent in vitro fertilization and culture in two media. Turk. J. Vet. Anim. Sci., 34: 33-38. 
  3. Chance, B., Sies. H. and Boveris. A. (1979). Hydroperoxide metabolism in mammalian organs. Physiol. Rev., 59: 527-605. 
  4. Coskun, S. and Lin. Y. C. (1993). Site of action of epidermal growth factor (EGF) on in-vitro porcine oocyte maturation. End. J., 1: 87-91.
  5. Dang, N. T. Q., Tich. N. K., Nguyen. B. X., Ozawa. M., Kikuchi. K., Manabe. N., Ratky. J., Kanai. Y. and Nagai. T. (2010). Introduction of various Vietnamese indigenous pig breeds and their conservation by using assisted reproductive techniques. J. Reprod. Dev., 56: 31-35.
  6. Das, K., Tagatz. G. E., Stout. L. E., Phipps. W. R., Hensleigh. H. C. and Leung. B. S. (1991). Direct positive effect of epidermal growth factor on the cytoplasmic maturation of mouse and human oocyte. Fertil. Steril., 55: 1000-1004.
  7. Ding J. and Foxcroft. G. R. (1994). Epidermal growth factor enhances oocyte maturation in pigs. Mol. Reprod. Dev., 39: 30-40.
  8. Downs, S. M. (1989). Specificity of epidermal growth factor action on maturation of the murine oocyte and cumulus oophorus in-vitro. Biol. Reprod., 41: 371-379.
  9. Downs, S. M., Dow. M. P. D. and Fagbohun. C. F. (1991). The meiotic response of cumulus cell-enclosed mouse oocyte to follicle stimulating hormone in the presence of different macromolecules. J. Exp. Zoo., 258: 373-383.
  10. Fenk, P., Knecht. M. and Catt. (1987). Hormonal control of epidermal growth factor receptors by gonadotropins during granulosa cell differentiation. Endocrinology, 120: 1121-1126.
  11. Food and Agricultural Organization of the United Nation (FAO). (2000). Globe regions-Breeds at risk/Global summary. In: B D S (ed), World Watch List for Domestic Animal Diversity 3rd (ed). Rome, Italy: Food Agric. Org., United Nations, 53-63.
  12. Gall, L., Boulesteix. C., Ruffini. D. and Germain. G. (2005). EGF-induced EGF receptor and MAP kinase phosphorylation in goat cumulus cells during in-vitro maturation. Mol. Reprod. Dev., 71: 489-494.
  13. Gall, L., Chene. N., Dahirel. M., Ruffini. D. and Boulesteix. C. (2004). Expression of epidermal growth factor receptor in the goat cumulus-oocyte complex. Mol. Reprod. Dev., 67: 439-445.
  14. Grupen, C. G., Nagashima. H. and Nottle. M. B. (1997). Role of epidermal growth factor and insulin-like growth factor-1 on procine oocyte maturation and embryonic development in-vitro. Reprod. Fertil. Dev., 9: 571-575.
  15. Hsu, C. J., Holmes. S. D. and Hammond. J. M. (1987). Ovarian epidermal growth factor-like activity. Concentration in porcine follicular fluid during follicular enlargement. Biochem. Biophys. Res. Comm., 147: 242-247.
  16. Kishida, R., Lee. E. S. and Fukui. Y. (2004). In-vitro maturation of porcine oocytes using a defined medium and developmental capacity after intracytoplasmic sperm injection. Theriogenology, 62: 1663-1676.
  17. Krisher, R. L. (2004). The effect of oocyte quality on development. J. Animal Sci.,82: 14-23.
  18. Kumar, D. and Purohit. G. N. (2004). Effect of epidermal and insulin like growth factor-I on cumulus expansion, nuclear maturation and fertilization of buffalo cumulus oocytes complexes in simple serum free media DMEM and Ham’s F10. Vet. Arhiv.,74: 13-25.
  19. Lorenzo, P. L., Illera. J. C. and Illera. M. (1995). Role of EGF, IGF-I and cumulus cells on maturation in-vitro of bovine oocytes. Theriogenology, 44: 109-118.
  20. Lorenzo, P. L., Illera. M. J., Illera. J. C. and Illera. M. (1994). Enhancement of cumulus expansion and nuclear maturation during bovine oocyte maturation in-vitro by the addition of epidermal growth factor and insulin-like growth factor. J Reprod Fertil., 101: 697-701.
  21. Marques, M. G., Nicacio. A.C., Oliveira. V. P., Nascimento. A. B., Caetano. H. V. A. C., Mendes. M., Mello. M. R. B., Milazzotto. M. P., Assumpc. M. E. O. and Visintin. J. A. (2007). In-vitro maturation of pig oocytes with different media, hormone and meiosis inhibitors. Anim. Reprod. Sci., 97: 375-381. 
  22. Martin, M. J. (2000). Development of in vivo matured porcine oocytes following Intracytoplasmic Sperm Injection. Biol. Reprod., 63: 109-112. 
  23. May, J. V., Buck. P. A. and Schomberg. D. W. (1987). Epidermal growth factor enhances iodo-follicle stimulating hormone binding by cultured porcine granulosa cells. Endocrinology, 120: 2413-2420.
  24. Merlo, B., Iacono. E., Zambelli. D., Prati. F. and Belluzzi. S. (2005). Effect of EGF on in-vitro maturation of domestic cat oocytes. Theriogenology, 7: 2032-2039.
  25. Nagar, D. and Purohit. G. N. (2005). Effect of epidermal growth factor on maturation and fertilization in vitro of goat folliclular oocytes in a serum free or serum supplemented medium. Vet. Arhiv., 75: 459-467.
  26. Oberlender, G., Murgas. L. D. S., Zangeronimo. M. G., Silva. A. C., Menezes. T. A. and Pontelo. T. P. (2013). Porcine follicular fluid concentration of free insulin-like growth factor-I collected from different diameter ovarian follicles. Pesq. Vet. Bras., 33: 1269-1274.
  27. Prochazka, R., Petlach. M., Nagyova. E.and. Nemcova. L (2011). Effect of epidermal growth factor-like peptides on pig cumulus cell expansion, oocyte maturation, and acquisition of developmental competence in-vitro: comparison with gonadotropins. Reproduction, 141: 425-435.
  28. Reed, M. L., Estrada. J. L., Illera M. J. M. and Petters. R. M. (1993). Effects of epidermal growth factor, insulin-like growth factor-I, and dialyzed porcine follicular fluid on porcine oocyte maturation in-vitro. J. Exp. Zool., 266: 74-78.
  29. Shimada, M., Hernandez-Gonzalez. I., Gonzalez-Robayna. I. and Richards. J. S. (2006). Paracrine and autocrine regulation of epidermal growth factor-like factors in cumulus oocyte complexes and granulosa cells: key roles for prostaglandin synthase 2 and progesterone receptor. Mol. Endocrinol., 20: 1352-13675.
  30. Singh, B., Rutledge. J. M. and Armstrong. D. T. (1995). Epidermal growth factor and its receptor gene expression and peptide localization in porcine ovarian follicles. Mol. Reprod. Dev., 40: 391-399.
  31. Sofi, K. A., Khan. M. L., Islam. R. and Lone. F. A. (2011). Effect of cysteamine and epidermal growth factor supplementation on the in-vitro maturation rate of swine oocytes. Small Ruminant Research., 96: 191-194.
  32. Wang, W. H. and Niwa. K. (1995). Effects of epidermal growth factor (EGF) and gonadotropins on cumulus expansion and nuclear maturation of pig oocytes in serum-free medium. Assist. Reprod. Technol. Androl., 7: 41-55.
  33. Xia, P., Tekpetey. F. R. and Armstrong. D. T. (1994). Effect of IGF-I on pig oocyte maturation, fertilization and early embryonic development in-vitro, and on granulosa and cumulus cell biosynthetic activity. Mol. Reprod. Dev., 38: 373-379. 
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Indian Journal of Animal Research
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    Research Article

    volume 52 issue 3 (march 2018) : 353-357,   Doi: 10.18805/ijar.B-3202

    Effect of growth factor on in-vitro maturation of porcine oocyte

    N
    N. Mahanta
    D
    D. Bhuyan
    S
    Suresh Kumar
    R
    R.K. Biswas
    D
    D.J. Dutta
    B
    B.C. Deka
    A
    A. Das
    R
    R.K. Dewry
    G
    G Hazarika
    1Department of Animal Reproduction, Gynaecology & Obstetrics, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati 781 022, Assam, India
    Cite article:- Mahanta N., Bhuyan D., Kumar Suresh, Biswas R.K., Dutta D.J., Deka B.C., Das A., Dewry R.K., Hazarika G (2018). Effect of growth factor on in-vitro maturation of porcine oocyte. Indian Journal of Animal Research. 52(3): 353-357. doi: 10.18805/ijar.B-3202.

    ABSTRACT

    The present study was aimed to evaluate the beneficial effect of different growth factors on in-vitro maturation of porcine oocytes. Ovaries were collected from a local abattoir immediately after slaughter of the animals and transported to the laboratory. A total of 618 type A and type B oocytes were cultured in TCM-199 containing additives with PMSG and  hCG for the first 22 hrs and without hormones for subsequent 22 hrs of incubation at 39o C under 5 per cent CO2 level and 90-95 per cent humidity. The effects of supplementation of different growth factors viz., EGF, IGF-I and EGF + IGF-I in the medium were studied. The rate of oocytes with cumulus cells expansion was significantly higher (P<0.01) when  growth factors were added as compared to control but it did not differ significantly between growth factors. The rate of nuclear maturation of oocytes was significantly higher (P<0.01) as compare to control for EGF and EGF + IGF-I but not for IGF-I. There was no significant difference in the rate of oocytes with nuclear maturation between the growth factors studied. It can be concluded from the present study that addition of EGF, IGF-I or EGF + IGF and additives along with hormones (PMSG and hCG for first 20-22 hrs) in TCM-199 Medium gives optimum in-vitro maturation rates in porcine oocytes.

    REFERENCES

    1. Abeydeera, L. R., Wang, W. H., Cantley, T.C., Rieke. A., Murphy. C. N. and Prather. R. S. (2000). Development and viability of pig oocytes matured in a protein-free medium containing epidermal growth factor. Theriogenology., 54: 787–797.
    2. Bastan, A., Polat. B., Acar. D. B., Korkmaz. O. and Colak. A. (2010). Determination of optimal dose of EGF for bovine oocyte maturation and subsequent in vitro fertilization and culture in two media. Turk. J. Vet. Anim. Sci., 34: 33-38. 
    3. Chance, B., Sies. H. and Boveris. A. (1979). Hydroperoxide metabolism in mammalian organs. Physiol. Rev., 59: 527-605. 
    4. Coskun, S. and Lin. Y. C. (1993). Site of action of epidermal growth factor (EGF) on in-vitro porcine oocyte maturation. End. J., 1: 87-91.
    5. Dang, N. T. Q., Tich. N. K., Nguyen. B. X., Ozawa. M., Kikuchi. K., Manabe. N., Ratky. J., Kanai. Y. and Nagai. T. (2010). Introduction of various Vietnamese indigenous pig breeds and their conservation by using assisted reproductive techniques. J. Reprod. Dev., 56: 31-35.
    6. Das, K., Tagatz. G. E., Stout. L. E., Phipps. W. R., Hensleigh. H. C. and Leung. B. S. (1991). Direct positive effect of epidermal growth factor on the cytoplasmic maturation of mouse and human oocyte. Fertil. Steril., 55: 1000-1004.
    7. Ding J. and Foxcroft. G. R. (1994). Epidermal growth factor enhances oocyte maturation in pigs. Mol. Reprod. Dev., 39: 30-40.
    8. Downs, S. M. (1989). Specificity of epidermal growth factor action on maturation of the murine oocyte and cumulus oophorus in-vitro. Biol. Reprod., 41: 371-379.
    9. Downs, S. M., Dow. M. P. D. and Fagbohun. C. F. (1991). The meiotic response of cumulus cell-enclosed mouse oocyte to follicle stimulating hormone in the presence of different macromolecules. J. Exp. Zoo., 258: 373-383.
    10. Fenk, P., Knecht. M. and Catt. (1987). Hormonal control of epidermal growth factor receptors by gonadotropins during granulosa cell differentiation. Endocrinology, 120: 1121-1126.
    11. Food and Agricultural Organization of the United Nation (FAO). (2000). Globe regions-Breeds at risk/Global summary. In: B D S (ed), World Watch List for Domestic Animal Diversity 3rd (ed). Rome, Italy: Food Agric. Org., United Nations, 53-63.
    12. Gall, L., Boulesteix. C., Ruffini. D. and Germain. G. (2005). EGF-induced EGF receptor and MAP kinase phosphorylation in goat cumulus cells during in-vitro maturation. Mol. Reprod. Dev., 71: 489-494.
    13. Gall, L., Chene. N., Dahirel. M., Ruffini. D. and Boulesteix. C. (2004). Expression of epidermal growth factor receptor in the goat cumulus-oocyte complex. Mol. Reprod. Dev., 67: 439-445.
    14. Grupen, C. G., Nagashima. H. and Nottle. M. B. (1997). Role of epidermal growth factor and insulin-like growth factor-1 on procine oocyte maturation and embryonic development in-vitro. Reprod. Fertil. Dev., 9: 571-575.
    15. Hsu, C. J., Holmes. S. D. and Hammond. J. M. (1987). Ovarian epidermal growth factor-like activity. Concentration in porcine follicular fluid during follicular enlargement. Biochem. Biophys. Res. Comm., 147: 242-247.
    16. Kishida, R., Lee. E. S. and Fukui. Y. (2004). In-vitro maturation of porcine oocytes using a defined medium and developmental capacity after intracytoplasmic sperm injection. Theriogenology, 62: 1663-1676.
    17. Krisher, R. L. (2004). The effect of oocyte quality on development. J. Animal Sci.,82: 14-23.
    18. Kumar, D. and Purohit. G. N. (2004). Effect of epidermal and insulin like growth factor-I on cumulus expansion, nuclear maturation and fertilization of buffalo cumulus oocytes complexes in simple serum free media DMEM and Ham’s F10. Vet. Arhiv.,74: 13-25.
    19. Lorenzo, P. L., Illera. J. C. and Illera. M. (1995). Role of EGF, IGF-I and cumulus cells on maturation in-vitro of bovine oocytes. Theriogenology, 44: 109-118.
    20. Lorenzo, P. L., Illera. M. J., Illera. J. C. and Illera. M. (1994). Enhancement of cumulus expansion and nuclear maturation during bovine oocyte maturation in-vitro by the addition of epidermal growth factor and insulin-like growth factor. J Reprod Fertil., 101: 697-701.
    21. Marques, M. G., Nicacio. A.C., Oliveira. V. P., Nascimento. A. B., Caetano. H. V. A. C., Mendes. M., Mello. M. R. B., Milazzotto. M. P., Assumpc. M. E. O. and Visintin. J. A. (2007). In-vitro maturation of pig oocytes with different media, hormone and meiosis inhibitors. Anim. Reprod. Sci., 97: 375-381. 
    22. Martin, M. J. (2000). Development of in vivo matured porcine oocytes following Intracytoplasmic Sperm Injection. Biol. Reprod., 63: 109-112. 
    23. May, J. V., Buck. P. A. and Schomberg. D. W. (1987). Epidermal growth factor enhances iodo-follicle stimulating hormone binding by cultured porcine granulosa cells. Endocrinology, 120: 2413-2420.
    24. Merlo, B., Iacono. E., Zambelli. D., Prati. F. and Belluzzi. S. (2005). Effect of EGF on in-vitro maturation of domestic cat oocytes. Theriogenology, 7: 2032-2039.
    25. Nagar, D. and Purohit. G. N. (2005). Effect of epidermal growth factor on maturation and fertilization in vitro of goat folliclular oocytes in a serum free or serum supplemented medium. Vet. Arhiv., 75: 459-467.
    26. Oberlender, G., Murgas. L. D. S., Zangeronimo. M. G., Silva. A. C., Menezes. T. A. and Pontelo. T. P. (2013). Porcine follicular fluid concentration of free insulin-like growth factor-I collected from different diameter ovarian follicles. Pesq. Vet. Bras., 33: 1269-1274.
    27. Prochazka, R., Petlach. M., Nagyova. E.and. Nemcova. L (2011). Effect of epidermal growth factor-like peptides on pig cumulus cell expansion, oocyte maturation, and acquisition of developmental competence in-vitro: comparison with gonadotropins. Reproduction, 141: 425-435.
    28. Reed, M. L., Estrada. J. L., Illera M. J. M. and Petters. R. M. (1993). Effects of epidermal growth factor, insulin-like growth factor-I, and dialyzed porcine follicular fluid on porcine oocyte maturation in-vitro. J. Exp. Zool., 266: 74-78.
    29. Shimada, M., Hernandez-Gonzalez. I., Gonzalez-Robayna. I. and Richards. J. S. (2006). Paracrine and autocrine regulation of epidermal growth factor-like factors in cumulus oocyte complexes and granulosa cells: key roles for prostaglandin synthase 2 and progesterone receptor. Mol. Endocrinol., 20: 1352-13675.
    30. Singh, B., Rutledge. J. M. and Armstrong. D. T. (1995). Epidermal growth factor and its receptor gene expression and peptide localization in porcine ovarian follicles. Mol. Reprod. Dev., 40: 391-399.
    31. Sofi, K. A., Khan. M. L., Islam. R. and Lone. F. A. (2011). Effect of cysteamine and epidermal growth factor supplementation on the in-vitro maturation rate of swine oocytes. Small Ruminant Research., 96: 191-194.
    32. Wang, W. H. and Niwa. K. (1995). Effects of epidermal growth factor (EGF) and gonadotropins on cumulus expansion and nuclear maturation of pig oocytes in serum-free medium. Assist. Reprod. Technol. Androl., 7: 41-55.
    33. Xia, P., Tekpetey. F. R. and Armstrong. D. T. (1994). Effect of IGF-I on pig oocyte maturation, fertilization and early embryonic development in-vitro, and on granulosa and cumulus cell biosynthetic activity. Mol. Reprod. Dev., 38: 373-379. 
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