Indian Journal of Animal Research

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Research Article

Indian Journal of Animal Research, volume 52 issue 2 (february 2018) : 208-211

Polymorphism of Prolactin gene in relation to egg production performance in Kadaknath hens

Shiv Kumar Yadav, S.K. Maurya, Alok Kumar Yadav, Kamalesh Yadav, K.D. Singh
1<p>Department of Veterinary Physiology &amp; Biochemistry, College of Veterinary Science &amp; Animal Husbandry<br /> Narendra Deva University of Agriculture &amp; Technology, Kumarganj, Faizabad- 224 229, Uttar Pradesh, India.</p>
Cite article:- Yadav Kumar Shiv, Maurya S.K., Yadav Kumar Alok, Yadav Kamalesh, Singh K.D. (2016). Polymorphism of Prolactin gene in relation to egg productionperformance in Kadaknath hens . Indian Journal of Animal Research. 52(2): 208-211. doi: 10.18805/ijar.11327.

ABSTRACT

Present study was carried out to study egg production performance and polymorphism of Prolactin gene at 24 bp indel locus at promoter region (PRL24). Egg production performances were recorded as age at first egg (AFE), Body Weight at First Egg (WFE), Mean Egg Weight (MEW) and Total No. of Eggs at 90 days of laying (TEN). DNA was isolated from blood of 20 Kadaknath birds collected from wing vein. PRL24 locus for indel polymorphism was amplified by PCR and the product was resolved on 6% native PAGE for genotyping. The AFE (d), WFE (Kg), MEW (g) and TEN of Kadaknath hens in the present study were found to be 188.00±0.71, 1.26±0.03, 42.83±0.21 and 37.75±0.59 respectively. The Prolactin gene locus PRL24 showed two alleles I and D and three genotypes: II, ID and DD. The frequencies of I and D alleles at this locus were 0.55 & 0.45 respectively. The birds of D allele had a significantly (P<0.05) better TEN than birds of I allele.

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