Indian Journal of Animal Research

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Indian Journal of Animal Research, volume 52 issue 1 (january 2018) : 173-174

Comparative efficacy of cryoprotectant in the lyophilization of pigeon paramyxovirus-1 vaccine

Mousam Das, Devi Prasad Isore, Chanchal Guha, Ujjwal Biswas, Amaresh Chatterjee
1<p>Department of Veterinary Microbiology, West Bengal University of Animal &amp;Fishery Sciences,&nbsp;37, K. B. Sarani, Kolkata- 700037, West Bengal, India.</p>
Cite article:- Das Mousam, Isore Prasad Devi, Guha Chanchal, Biswas Ujjwal, Chatterjee Amaresh (2017). Comparative efficacy of cryoprotectant in the lyophilizationof pigeon paramyxovirus-1 vaccine . Indian Journal of Animal Research. 52(1): 173-174. doi: 10.18805/ijar.v0iOF.7656.

ABSTRACT

Preparation of live attenuated freeze dried Pigeon Paramyxo Virus-1 (PPMV-1) vaccine selection of proper cryoprotectant which could preserve and protect the virus against injury due to reduction of temperature at the time of lyophilization, was very necessary. PPMV-1 locally isolated lentogenic strain was selected as seed virus which was properly attenuated by serially passaging in 9 to 10 days old embryonated specific pathogen free (SPF) fowl eggs and produced master seed virus and working seed virus. Five per cent lactalbumin hydrolysate with 10% sucrose (LAS) and 1% polyvinyl pyrrolidone (PVP) were used as two different cryoprotectant for lyophilization of working seed virus. After lyophilization value of haemagglutination (HA) titer and embryo infective dose fifty per ml (EID50/ml) were changed and concluded that both LAS and PVP could be used as suitable cryoprotectant where LAS was better than PVP which was first time done in the whole world.

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