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Comparative efficacy of cryoprotectant in the lyophilization of pigeon paramyxovirus-1 vaccine

Mousam Das, Devi Prasad Isore, Chanchal Guha, Ujjwal Biswas, Amaresh Chatterjee

Department of Veterinary Microbiology, West Bengal University of Animal &Fishery Sciences, 37, K. B. Sarani, Kolkata- 700037, West Bengal, India.

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Preparation of live attenuated freeze dried Pigeon Paramyxo Virus-1 (PPMV-1) vaccine selection of proper cryoprotectant which could preserve and protect the virus against injury due to reduction of temperature at the time of lyophilization, was very necessary. PPMV-1 locally isolated lentogenic strain was selected as seed virus which was properly attenuated by serially passaging in 9 to 10 days old embryonated specific pathogen free (SPF) fowl eggs and produced master seed virus and working seed virus. Five per cent lactalbumin hydrolysate with 10% sucrose (LAS) and 1% polyvinyl pyrrolidone (PVP) were used as two different cryoprotectant for lyophilization of working seed virus. After lyophilization value of haemagglutination (HA) titer and embryo infective dose fifty per ml (EID50/ml) were changed and concluded that both LAS and PVP could be used as suitable cryoprotectant where LAS was better than PVP which was first time done in the whole world.

Cryoprotectant, Lactalbumin hydrolysate, Lyophilization, Polyvinyl pyrrolidone, Sucrose.
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