Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

  • Print ISSN 0367-6722

  • Online ISSN 0976-0555

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Indian Journal of Animal Research, volume 52 issue 1 (january 2018) : 51-55

Nucleotide variability in Beta 2 Microglobulin (b2M) gene and its association with colostral IgG levels in buffaloes (Bubalus bubalis)

Rajni Chaudhary, Subodh Kumar, H.M. Yathish, A. Sivakumar, Chinmoy Mishra, Amit Kumar, Anuj Chauhan, B. Sivamani, N.R. Sahoo
1<p style="text-align: justify;">Division of Animal Genetics,&nbsp;ICAR-Indian Veterinary Research Institute, Breilly-243 122, Uttar Pradesh, India.&nbsp;</p>
Cite article:- Chaudhary Rajni, Kumar Subodh, Yathish H.M., Sivakumar A., Mishra Chinmoy, Kumar Amit, Chauhan Anuj, Sivamani B., Sahoo N.R. (2016). Nucleotide variability in Beta 2 Microglobulin (b2M) gene and its association with colostral IgG levels in buffaloes (Bubalus bubalis) . Indian Journal of Animal Research. 52(1): 51-55. doi: 10.18805/ijar.v0iOF.4566.

The present study was taken up to find out Beta 2 Microglobulin (b2M) gene variants and their association with IgG concentrations in colostrum of Murrah buffaloes. 40 newly parturated Murrah buffaloes dams were included in the present study. Polymerase chain reaction- single stranded conformational polymorphism (PCR-SSCP) technique was used to explore the polymorphism in b2M gene of buffaloes. The PCR-SSCP analysis and sequencing of different patterns obtained by silver staining of fragments pertaining to exon 1 through 4 and partial introns 1 through 3, revealed 9 SNPs which were further used to prepare the haplotypes. 7 different haplotypes were observed. Indirect ELISA was done to estimate IgG concentrations in colostrum (collected 1 hour before first milking) obtained from buffaloes. IgG levels in colostrum ranged from 11.22 to 185.1 mg/ml with a mean IgG concentration of 51.71 ± 5.99 mg/ml. The effect of different haplotypes was evaluated on IgG concentrations. The least square analysis of variance could not exhibit a significant effect of buffalo dam haplotype on colostral IgG concentration.

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