Ameliorating aflatoxicosis in commercial broiler chickens by dietary Mycosorb: Heamato-Biochemical studies

DOI: 10.18805/ijar.B-3182    | Article Id: B-3182 | Page : 46-50
Citation :- Ameliorating aflatoxicosis in commercial broiler chickens by dietary Mycosorb: Heamato-Biochemical studies.Indian Journal of Animal Research.2018.(52):46-50
M.R. Wade, D. Sapcota and Urwashi Verma drmangeshwade@gmail.com
Address : Department of Poultry Science, Collage of Veterinary Science, Assam Agricultural University, Khanapara-781 022, Assam, India.
Submitted Date : 5-02-2016
Accepted Date : 13-05-2017


A study was conducted to find out the ameliorating effect of dietary Mycosorb (esterified glucomannan, EGM) in experimental aflatoxicosis of broilers. For this purpose 270 number of day-old Anak 2000 chicks were procured and divided into 6 equal treatment groups. A control ration (T0) was prepared with conventional feedstuffs and an experimental diet (Tx) was prepared from this ration after incorporating aflatoxin (AF) @ 300 ppb produced on rice using Aspergillus flavus: NRRL 2999 strain through fermentation technology. From Tx three rations were prepared by mixing Mycosorb @0.025 per cent of feed (TxM1), 0.50 per cent of feed (TxM2) or 0.10 per cent of feed (TxM3). A positive control diet (T0 M2) was prepared by mixing Mycosorb @ 0.5 per cent in the control ration. All 6 diets were fed to the respective groups for a period 42 days. Significantly (P<0.05) reduced level of serum cholesterol and serum protein levels were observed in aflatoxin-alone fed group; whereas increase surge of glucose, triglyceride and activities of serum enzymes viz., serum glutamate oxaloacetate transaminase, serum glutamate pyruvate transaminase, alkaline phosphatase were recorded in the aflatoxin-alone fed group. However, the increased parameters were corrected to normalcy when Mycosorb was added in the contaminated diet, particularly at 0.1 per cent level. The study showed an ameliorating effect of dietary Mycosorb during experimental aflatoxicosis.


Aflatoxin Broiler chicken Heamato-biochemical parameter Mycosorb.


  1. Aravind, K.L., Patil, V.S., Devegowda, G., Umakantha, B. and Ganpule, S.P. (2003). Efficacy of esterified glucomannan to counteract mycotoxicosis in naturally contaminated feed on performance and serum biochemical, haematological parameters in broilers. Poultry Science, 82: 571–576.
  2. Banlunara, W., Bintvihok, A. and Kumagai, S. (2005). Immunohistochemical study of proliferating cell nuclear antigen in duckling liver fed with aflatoxin B1 and esterified glucomannan. Toxicon, 46: 954-957.
  3. Basmacoglu, H., Oguz, H., Ergu, M., Col, R. and Brdane, Y.O. (2005). Effect of dietary esterified glucomannan on performance, serum biochemistry and hematology in broiler exposed to aflatoxin. Czech Journal of Animal Science, 50 (1): 31-39.
  4. BIS, (1992). Nutrient requirements of Poultry. Bureau of Indian Standards Manak Bhavan, New Delhi. 
  5. Cao, J. and Wang, W. (2014). Effects of astaxanthin and esterified glucomannan on hematological and serum parameters, and liver pathological changes in broilers fed aflatoxin-B1-contaminated feed. Animal Science Journal, 85(2):150-157.
  6. Che, Z., Liu, Y., Wang, H., Zhu, H., Hou, Y. and Ding, B. (2011). The Protective Effects of Different Mycotoxin Adsorbents against Blood and Liver Pathological Changes Induced by Mold-contaminated Feed in Broilers. Asian- Australasian Journal of Animal Sciences, 24(2): 250 – 257. 
  7. Chibanga, J.F., Banda-Nyirenda, D., and Simbaya, J. (2014). Effects of Supplementing Different Levels of Vitamin A to Aflatoxin B1 Contaminated Diets on the Performance of Broiler Chickens. Asian Journal of Natural and Applied Science, 3(4) 35-46. 
  8. Diaz, D.E. and Smith, T.K. (2005). Mycotoxin sequestering agents: Practical tools for neutralisation of mycotoxins. In: The Mycotoxin Blue Book, Diaz D.E. (ed.), Nottingham University Press, Nottingham pp: 323-339.
  9. Diaz, D.E., Hagler, W.M., Hopkins, B.A. and Whitlow, L.W. (2002). Aflatoxin binders I: in vitro binding assay for aûatoxin B1 by several potential sequestering agents. Mycopathologia, 15(6): 223–226. 
  10. Ghahri, H., Reza, H. and Mehdi, A.F. (2010). Evaluation of the efficacy of esterify glucomannan, sodium bentonite, and humic acid to ameliorate the toxic effects of aflatoxin in broilers .Turkish Journal of Veterinary and Animal Science, 34(4): 385-391.
  11. Girish, C.K., and Devegowda, G. (2006). Efficacy of Glucomannan-containing Yeast Product (Mycosorb) and Hydrated Sodium Calcium Aluminosilicate in Preventing the Individual and Combined Toxicity of Aflatoxin and T-2 Toxin in Commercial Broilers. Asian-Australasian Journal of Animal Sciences, 19: 877–883.
  12. Huff, W.E., Kubena, L.F., Harvey, R.B., Corrier, D.E. and Mollenhauer, H.H. (1986). Procession of aflatoxicosis in broiler chickens. Poultry Science, 65: 191- 199.
  13. Kana, J.R., Ngoula, F., Tchoffo, H., Tadondjou, C.D., Sadjo, Y.R., Teguia, A. and Gbemenou, J. B. (2014). Effect of biocharcoals on hematological, serum biochemical and histological parameters in broiler chickens fed aflatoxin B1-contaminated diets. J. Animal Science Advances, 4(7): 939-948
  14. Khatke, P.A., Singh Ram., Mandal, A.B. and Tyagi, P.K. (2013). Ability of biological adsorbents to ameliorate aflatoxicosis in broiler chicken: Effect on blood biochemicals and organ weights. Indian Journal of Poultry Science, 48(1):117-121.
  15. Kumar, C. B., Reddy, B.S.V., Gloridoss, R.G., Prabhu, T.M., Wodeyar, D.S. and Suresh, B.N. (2016)Efficacy of biotechnologically derived product in counteracting ill effects of aflatoxins in broiler chicken. Indian Journal of Animal Research, 50 (1): 75-79
  16. Kumar, Dhanapal S., Rao, S., Govindaraju, P.K.P., Hukkeri, R. and Mathesh, K. (2014). Ameliorative efficacy of citrus fruit oil in aflatoxicosis in broilers: a growth and biochemical study. Turkish Journal of Veterinary and Animal Sciences, 38: 207-211.
  17. Lakkawar, A.W., Sathyanarayana, M.L., Narayanaswamy, H.D., Sugunarao., Yathiraj, S., Isloor, S.K., Shridhar, N.B.and Krishnaveni, N.(2016) Efficacy of diatomacious earth in amelioration of aflatoxin induced toxicity in broiler chicken. Indian Journal of Animal Research, .50 (4): 529-536
  18. Maldhure, N. A. and Churchil, R. R.(2015).Effect of dietary esterified glucomannan on performance of broilers exposed to aflatoxin. Indian Journal of Animal Research, 49 (5) : 658-661
  19. Mani, K., Sundaresan, K.and Viswanathan, K. (2000). Effect of dietary aflatoxin B1 on the blood constituents in commercial broilers. Indian Journal of Poultry Science, 35: 176-180. 
  20. Motawe, H.F.A., Abdel Salam, A.F. and El Kh, M. (2014). Reducing the toxicity of aflatoxin in broiler chickens’ diet by using probiotic and yeast. International Journal of Poultry Science, 13 (7): 397-407.
  21. Oguz, H., F. Kurtoglu, V. Kurtoglu, and Y.O.Birdane (2002). Evaluation of biochemical characters of broiler chickens during dietary aflatoxin (50 and 100 ppb) and clinoptilolite exposure. Research in Veterinary Science, 73:101-103.
  22. Oguz, H., Kecec, T., Birdane, Y.O., Onde, R.F. and Kurtoglu, V. (2000). Effect of clinoptilolite on serum biochemical and haematological characters of broiler chickens during experimental aflatoxicosis. Research in Veterinary Science, 69: 89–93.
  23. Romer, T. R. (1975). Screening method for the detection of aflatoxins in mixed feed and other agricultural commodities with subsequent confirmation and quantitative measurement of aflatoxins in positive samples. Journal of the Association Official Analytical Chemists, 58: 500-06.
  24. Rosa, C.A.R., Miazzo, R., Magnoli, C., Salvano, M., Chiacchiera, S.M., Ferrero, S., Saenz, M., Carvalho, E.C.Q. and Dalcero, A. (2001). Evaluation of the efficacy bentonite from the south of Argentina to ameliorate the toxic effects of aflatoxin in broilers. Poultry Science, 80: 139–144.
  25. Sapcota, D., Islam, R. and Upadhyaya, T.N. (2006c). Experimental aflatoxicosis in commercial broilers and its amelioration by deity Andrographis paniculata: A haemato- pathological study. Animal Nutrition and Feed Technology, 6: 177- 184.
  26. Sapcota, D., Islam, R., Upadhyaya, T.N. and Borah, M. (2006b). Induced aflatoxicosis and its amelioration by dietary Piper nigurm and methomin in broiler chickens: Haemato-biochemical organ weight and leg/feather score studies. Indian Journal of Animal Science, 76 (5): 408-412.
  27. Sapcota, D., R. Islam, and T.N. Upadhyaya (2006a). Dietary supplementation of Emblica officinalis for amelioration of experimental aflatoxicosis in commercial broilers. Animal Nutrition and Feed Technology, 6: 65-71.
  28. Shatwell, D., Hesseltine, L., Stubblefield, C.W. and Sorensorwg, R.D. (1966). Production of aflatoxin on rice. Applied Microbiology, 14: 425-428.
  29. Snedecor, G.W.,and Cochran, W.G. (1994). Statistical methods, 8th ed., (Iowa State University Press, Ames, Iowa, USA.
  30. Suksombat, W., Suksombat, P. and Mirattanaphrai, R. (2011). Effect of commercial or bovine yeasts on the performance and blood variables of broiler chickens intoxicated with aflatoxins. World Academy of Science, Engineering and Technology, 5: 10-22. 
  31. Thaxton, J.P., Tung, H.T. and Hamilton, P.B. (1974). Immunosuppression in chickens by aflatoxin. Poultry Science, 53 (2): 721-5. 
  32. Tingting, L., Shibin, Y., Zejun, Z. and Guiping, Y. (2015).Protective effects of sodium selenite against aflatoxin B1 on haemoglobin content, erythrocyte count, and immune adherence function in broilers Indian Journal of Animal Research,49 (3) : 360-363
  33. Valchev, I., D. Kanakov., L. Ts. HristovLazarov, R. Binev, N. Grozeva, and Y. Nikolov (2014). Investigations on the liver function of broiler chickens with experimental aflatoxicosis. Bulgarian Journal of Veterinary Medicine, 17(4): 302-13. 
  34. Verma, J., Swain, B.K. and Johri, T.S. (2012). Effect of aflatoxin and ochratoxin a on biochemical parameters in broiler chickens. Poultry Science, 91 (11):2792-2801. 
  35. Wang, R.J., Fui, S.X., Miao, C.H. and Feng, D.Y. (2006). Effects of different mycotoxin adsorbents on performance, meat characteristics and blood profiles of avian broilers fed mold contaminated corn. Asian-Australasian Journal of Animal Sciences, 19(1): 72-79. 
  36. Yildirim, E., Yalçinkaya, I., Kanbur, M., Cinar, M. and Oruç, E. (2011). Effects of yeast glucomannan on performance, some biochemical parameters and pathological changes in experimental aflatoxicosis in broiler chickens. Revue de Medecine Véterinaire, 162:8-    9, 413-420 

Global Footprints