Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

  • Print ISSN 0367-6722

  • Online ISSN 0976-0555

  • NAAS Rating 6.50

  • SJR 0.263

  • Impact Factor 0.4 (2024)

Frequency :
Monthly (January, February, March, April, May, June, July, August, September, October, November and December)
Indexing Services :
Science Citation Index Expanded, BIOSIS Preview, ISI Citation Index, Biological Abstracts, Scopus, AGRICOLA, Google Scholar, CrossRef, CAB Abstracting Journals, Chemical Abstracts, Indian Science Abstracts, EBSCO Indexing Services, Index Copernicus
Submit

Research Article

Indian Journal of Animal Research, volume 52 issue 1 (january 2018) : 20-24

Effect of different levels of egg-yolk on freezability of Jakhrana buck semen

Narendra Kumar, B. Rai, Chetna Gangwar, S.A. Lone, Anshuman Kumar, S.K. Gupta, Vipin Maurya
1<p>Department of Physiology Reproduction and Shelter Management,&nbsp;ICAR- Central Institute for Research on Goats, Makhdoom, Mathura-281 122, Uttar Pradesh, India.</p>
Cite article:- Kumar Narendra, Rai B., Gangwar Chetna, Lone S.A., Kumar Anshuman, Gupta S.K., Maurya Vipin (2016). Effect of different levels of egg-yolk on freezability of Jakhrana buck semen . Indian Journal of Animal Research. 52(1): 20-24. doi: 10.18805/ijar.11326.

ABSTRACT

The present study was designed to determine the effect of different levels of egg-yolk on freezability of Jakhrana buck semen. Six healthy Jakhrana bucks (BW=30 ± 2kg, age=12 ± 0.5 month) were used for semen collection. These bucks were maintained under semi-intensive system at Jakhrana Unit of C.I.R.G. Makhdoom, Mathura. A total of 48 ejaculates (6 bucks × 8 replicates) were collected twice a week using artificial vagina. Each ejaculate was divided into 4 groups (G1, G2, G3 and G4). The G1, G2, G3 and G4 were extended with Tris-egg yolk-citric acid- fructose-glycerol (TEYCFG) extenders containing 5, 10, 15 and 20% egg yolk level, respectively. Each ejaculate was evaluated for sperm motility, viability, abnormality, and hypo-osmotic swelling (HOS) response and acrosome integrity before and after freezing. At pre-freeze stage no significant (P>0.05) difference in sperm motility and viability was found among all groups. Sperm abnormality was significantly (P<0.05) higher in G4 as compared to other groups (G1, G2, G3). The HOS response and acrosomal integrity was significantly (P<0.05) higher in G1, G2 and G3 as compared to G4. However, no significant (P>0.05) difference was observed in HOS response and acrosomal integrity among G1, G2 and G3. At post thaw stage, sperm motility, viability and HOS response was significantly (P<0.05) higher in G1 and G2 as compared to G3 and G4. Sperm abnormality was significantly (P<0.05) lower in G2 as compared to other groups. The acrosomal integrity was significantly (P<0.01) higher in G1 and G2 as compared to G3 and G4. It is concluded that 10% egg yolk in Tris based extender may be the best for successful cryopreservation of Jakhrana buck semen. 

REFERENCES


  1. Ahangari, Y. J., Hamedani, M. A. and Hassani, S. R. (2010). Effect of various concentrations of egg yolk on Dallagh Ram semen characteristics at 5ºC. Aust. Soc. Anim. Prod., 28:88.

  2. Beran, J., Stádník, L. F., Bezdíèek, L., Louda, Cítek, J., and Duchácek, J. (2012). Effect of sire and extender on spermatozoa motility and share of live or dead sperm in bulls’ fresh ejaculate and in AI doses after thawing. Archiv Tierzucht., 55: 207-218.

  3. Bispo, C. A. S., Pugliesi, G., Galvaoc, P., Rodriguesb, M. T., Kerb, P. G., Filgueiras, B. and Carvalhob, G. R. (2011). Effect of low and high egg yolk concentrations in the semen extender for goat semen cryopreservation. Small Rumin. Res., 100: 54-58.

  4. C. Aurich. (2005). Factors affecting the plasma membrane function of cooled-stored stallion spermatozoa Anim. Reprod. Sci., 89: 65-75.

  5. Cabrera, F., Gonzalez, F., Batista, M., Calero, P., Medrano, A., and Gracia, A. (2005).The effect of removal of seminal plasma, egg yolk level and season on sperm freezability of Canary buck (Capra hircus). Reprod. Dom. Anim., 40: 191-195.

  6. Campbell, R.C., Hancock, J. L. and Rothschild, L. (1953). Counting live and dead bulls spermatozoa. J. Exp. Biol., 30: 44-49.

  7. De Leeuw, F. E., De Leeuw, A. M., Den Dass, J. H. and Colenbrander, V. (1993). Effects of various cryoprotective agents and membrane-stabilizing compounds on bull sperm membrane integrity after cooling and freezing. Cryobiology, 30 :32-44.

  8. Evans G and Maxwell WMC. (1987). Salamon’s Artificial Insemination of Sheep and Goats. Butterworths, Sydney, p. 194.

  9. Evans, R.W., and Setchell, B. P. (1978). Association of exogenous phospholipids with spermatozoa. J. Reprod. Fertil. 53: 357-362.

  10. Farstard W. (1996). Semen cryopreservation in dogs and foxes. Anim. Reprod.Sci., 42 : 251-260.

  11. Gangwar, C., Ranjan, R., Kumar, S., Kharche, S. D., Goel, A. K., Ramachandran, N. and Jindal, S. K. (2014) Use of chelating agent for optimum post thaw quality of buck semen. Indian J. Anim. Sci., 84: 8.

  12. Gundogan, M., Avdatek, F. and Yen, D. (2011). Effect of extenders on motility, morphology and osmotic resistance parameters of ram sperm during liquid storage. Rev. Méd. Vét. 162: 546-551.

  13. Juyena, N. S. (2011) Protein profiles and biochemical characteristics of semen: Influence on frozen thawed spermatozoa quality in rams (Ovis Aries) and Alpacas (Vicugna Aacos). PhD Thesis, Faculty of Veterinary Medicine, Department of Clinics Veterinary Science. University of Paduva, Italy, pp. 123-173.

  14. Kampschmidt, R. F., Mayer, D. T. and Herman, H. A. (1953). Lipid and lipoprotein constituent of egg yolk in the resistance and storage of bull spermatozoa. J. Dairy Sci., 36 : 733-742.

  15. Kharche, S.D., Jindal, S. K., Priyadharsini, R., Satish Kumar, Goel, A.K., Ramachandran, N. and Rout, P. K. (2013). Fertility following frozen semen artificial insemination in Jamunapari goats. Indian J. Anim. Sci., 83: 1071-1073.

  16. Leboeuf, B., Restall, B. and Salamon, S. (2000). Production and storage of goat semen for artificial insemination. Anim. Reprod. Sci., 62: 113-141

  17. Mazur, P. (1984). Freezing of living cells: mechanism and implications. Am. J. Physiol., 247: 125-142.

  18. Moussa, M., Matinet, V., Trimeche, Tainturier, A. D. and M. Anton. (2002). Low density lipoproteins extracted from hen egg yolk by an easy method: cryoprotective effect on frozen-thawed bull semen. Theriogenology. 57:1695–706.

  19. Priyadharsini, R., Jindal, S. K., Sharma, D., Ramachandran, N., Kharche, S. D. and Goel, A. K. (2011). Effect of different egg yolk level on the cryopreservation capability of Jakhrana goat semen. J. Anim. Sci. Adv., 1: 28-37.

  20. Quinn, P. J., Chow, P.Y.W. and White, I.G. (1980). Evidence that phospholipid protects ram spermatozoa from cold shock at plasma membrane site. J. Reprod. Fertil. 60 : 403-407.

  21. Ranjan, R., Ramchandran, N., Jindal, S. K., Goel, A. K. and Kharche, S. D. (2009). Effect of egg yolk levels on keeping quality of Marwari buck semen at refrigeration temperature. Indian J. Anim. Sci., 79: 662-664.

  22. Ranjan, R., Goel, A. K., Ramchandran, N., Kharche, S. D., and Jindal, S. K. (2015). Effect of egg yolk levels and equilibration periods on freezability of jamunapari buck semen. Indian J. Small Rumin., 21: 32-36

  23. Ray, kalyani., Gojen, L. Singh., and Bidhan, Sarkar. (2015). Cryopreservation of Black Bengal buck semen by Tris-based extenders containing different levels of egg-yolk. Annals of Vet. Anim. Sci., 2: 47-54.

  24. Salamon, S. and Maxwell, W.M.C. (2000). Storage of ram semen. Anim. Reprod. Sci. 62 : 77–111.

  25. Schneider, U. and Mazur, P. (1984). Osmotic consequences of cryoprotectant permeability and its relation to survival of frozen-thawed embryos. Theriogenology, 21: 68-79.

  26. Sharma, M., Singh, M., Kapoor, S. and Jaiswal, S. (2012). Inter relationship between some routine semen evaluation parameters in Jersey×local hill cattle crossbred bulls. Open Vet. J., 2: 26-31.

  27. Simpson A.M., Swan M.A., and White, I.G. (1986). Action of phoshatidylcholine in protecting ram sperm from cold shock. Gamete Research, 15: 43-56.

  28. Watson, P.F. (1975). Use of a Giemsa stain to detect changes in acrosomes of frozen spermatozoa. The Vet. Record., 97: 12-15. 

  29. Yimer, N., Noraisyah, A. H., Rosnina, Y., Wahid, H., Sarsaifi, K., and Hafizal, A. M. (2014). Comparison of cryopreservative effect of different levels of omega-3 egg-yolk in citrate extender on the quality of goat spermatozoa. Pak. Vet. J., 34: 347-350.

  30. Vyas, S., Mohan, G., Dhami, A.J. and Sahni, K. L. (1992). Studies on the norms and the correlations of initial and post thaw seminal attributes of triple crossbred bulls. Int. J. Anim. Sci., 7: 73-76.

     
Reviewed By

Download Article
In this Article
    Contact us
    Follow us

    Editorial Board

    View all (0)