Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

  • Print ISSN 0367-6722

  • Online ISSN 0976-0555

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Indian Journal of Animal Research, volume 50 issue 2 (april 2016) : 224-229

Analysis of bovine viral diarrhoea virus in Korea dairy farms

Hong-Je Park1, 2, Gyeong-Dong Kim1, Chi-Ho Lee2, Myung-Hwa Kang3, Kwan-Sik Min*
1<p>Animal Biotechnology, Graduate School of Future Convergence Technology,&nbsp;Institute of Genetic Engineering, Hankyong National University, Ansung 17579, Republic of Korea.</p>
Cite article:- Park1 Hong-Je, 2, Kim1 Gyeong-Dong, Lee2 Chi-Ho, Kang3 Myung-Hwa, Min* Kwan-Sik (2016). Analysis of bovine viral diarrhoea virus in Korea dairy farms . Indian Journal of Animal Research. 50(2): 224-229. doi: 10.18805/ijar.9374.

A total of 2,194 bulk-tank milks from dairy farms were investigated, and of which 842 dairy farms (55,263 cattle) were assessed to detect persistently infected (PI) individual cattle by performing ELISA. Reverse transcription (RT)-PCR and immunohistochemistry (IHC) were used to develop a rapid screening test for detecting Bovine viral diarrhoea virus (BVDV) in ear tissues. Testing for the bulk-milk tank from dairy farms showed that 2,007 (91.5%) out of a total of 2,194 farms were determined to be positive for BVDV, while only 187 bulk-milk tanks (8.5%) were negative. A total of 55,263 cattle were tested for BVDV, of which 669 (1.2%) cattle from 387 farms (46%) were identified as PI. The distribution of PI was 178 (65.4%) of 272 herds under 14 months. A 220 (80.9%) herds were found in less than 23 month ages. Four infected herds were identified as PI based on RT-PCR analysis. BVDV protein was shown to be localized within epidermal hair bulb cells. This approach of targeted testing of dairy herds using ELISA and RT-PCR for pre-diagnostic testing proves to markedly reduce BVDV-infected herds in Korean dairy farms and in Korean Native Cows. 

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