Indian Journal of Animal Research

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Research Article

Indian Journal of Animal Research, volume 51 issue 5 (october 2017) : 962-966

Detection of cow milk paneer in mixed/buffalo milk paneer through conventional species specific Polymerase Chain Reaction

Tanmay Hazra, Vivek Sharma, Rekha Sharma, S.De, Sumit Arora, Darshan Lal
1<p>ICAR-National Dairy Research Institute,&nbsp;Karnal-132 001, India.</p>
Cite article:- Hazra Tanmay, Sharma Vivek, Sharma Rekha, S.De, Arora Sumit, Lal Darshan (2016). Detection of cow milk paneer in mixed/buffalo milk paneer through conventional species specific Polymerase Chain Reaction . Indian Journal of Animal Research. 51(5): 962-966. doi: 10.18805/ijar.9491.

ABSTRACT

Due to higher market demand of buffalo milk paneer, lower price cow milk is often adulterated with higher cost buffalo milk for preparation of paneer. Till date no rapid technique is available in market to ensure that paneer is made from buffalo milk. Currently a PCR based method has been developed to authenticate the buffalo milk paneer. DNA was isolated from paneer by DNeasy Mericon food kit. A set of bovine specific primers (P1) targeting D-loop (displacement loop) of                            mt- DNA was selected and standardized to amplify cow DNA resulted 126bp amplicon. Using this PCR based approach even upto 1% level of cow milk adulteration in buffalo milk paneer could be detected. 

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