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Screening and evaluation of antioxidant and anticoccidial properties of condensed tannins containing tree leaves of Jammu province

Mohd. Aqib Zargar, A. K. Pathak and Mohd. Iqbal Daing

Division of Animal Nutrition, Faculty of Veterinary Sciences & Animal Husbandry, SKUAST-J, R. S. Pura, Jammu-181 102 (J & K), India.

dranand_pathak@yahoo.com

Page Range:
1101-1104
Article ID:
B-3151
Online Published:
25-04-2017
Abstract

Five promising tanniferous tree leaves viz. Acacia nilotica, Eugenia jambolana, Ficus religiosa, L. leucocephala and Psidium guajava were selected for in vitro antioxidant and anticoccidial properties against avian Eimeria species. Condensed tannins (CT) extracts of above tree leaves were prepared in 70 % acetone and then freeze dried in lyophilizer. Antioxidant property of lyophilized acetone extract of different CT sources was evaluated by in-vitro methods. The 2, 2-diphenyl-1- picryl-hydrazyl (DPPH) free radical scavenging activity was significantly higher in P. guajava as compared to other CT sources. However, hydrogen peroxide (H2O2) scavenging activity was significantly (P<0.05) lower in L. leucocephala compared to other CT sources. The CT extract of A. nilotica, E. jambolana, F. religiosa and P. guajava showed significantly (P<0.05) higher reducing power than that of L. leucocephala. Sporulation inhibition (%) of Eimeria spp. was significantly (P<0.05) higher in E. jambolana and P. guajava, followed by A. nilotica, L. leucocephla and least in F. religiosa, while, E. jambolana and P. guajava showed maximum sporulation inhibition activity @ 4 mg/ml. It was concluded that CT extracts of A. nilotica, E. jambolana, F. religiosa, L. leucocephala and P. guajava leaves possess the antioxidant and anti-coccidian property. It may be eco-friendly socioeconomic alternative, natural antioxidant, anti-coccidian agent and/ or natural feed additive for organic meat production.

Keywords
Anti-coccidian; Antioxidants; Condensed tannins extract; Tree leaves
References
  1. Abbas, R. Z., Iqbal, Z., Khan, M. N., Zafar, M. A. and Zia, M. A. (2010). Anticoccidial activity of Curcuma longa L. in Broiler Chickens. Braz. Arch. Biol. Technol. 53: 63–67.
  2. AOAC. (2000). Official methods of analysis (16th ed.). Association of Official Analytical Chemists. Washington, DC.
  3. Atawodi, S. E. (2005). Antioxidant potential of African medicinal plants. Afr J Biotechnol. 4 (2): 128-133. 
  4. Awais, M. M., Akhtar, M., Muhammad, F., Haq, A. U. and Anwar, M. I. (2011). Immuno therapeutic effects of some sugar cane (Saccharum officinarum L.) extracts against coccidiosis in industrial broiler chickens. Exp. Parasitol. 128: 104–110.
  5. Barrau, E., Fabre, N., Fouraste, I. and Hoste, H. (2005). Effect of bioactive compounds from sainfoin (Onobrychis viciifolia Scop.) on the in vitro larval migration of Haemonchus contortus: role of tannins and flavonol glycosides. Parasitol. 131: 531-538.
  6. Braca, A. T., Nunziatina, D. B., Lorenzo, D.I., Pizza, C., Politi, M. and Morelli, I. (2001). Antioxidant principles from Bauhinia terapotensis. J. Nat. Prod., 64: 892-895.
  7. Chew, Y. L., Goh, J. K. and Lim, Y. Y. (2009). Assessment of in-vitro antioxidant capacity and polyphenolic composition of selected medicinal herbs from Leguminosae family in Peninsular Malaysia. Food Chem. 19: 373-378.
  8. Dalloul, R. A. and Lillehoj, H. S. (2006). Poultry coccidiosis: r ecent a dvancements in control measures and vaccine development. Expert Rev. Vac. 5: 143-163.
  9. Dey, A., Dutta, N., Sharma, K. and Pattanaik, A. K. (2006). Evaluation of condensed tannins from tropical tree leaves and its impact on in vitro nitrogen degradability of groundnut cake. Anim. Nutr. Feed Tech. 6 (2): 215-222.
  10. Dinis, T.C. P., Madeira, V.M.C. and Almeida, L.M. (1994). Action of phenolic derivatives (acetaminophen, salicylate, and 5-    aminosalicylate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers. Arch. Biochem. Biophys. 315: 161-169.
  11. Dubey, M., Dutta, N., Banerjee, P. S., Pattanaik, A. K., Sharma, K. and Singh, M. (2012). Effect of condensed tannins supplementation through a tree leaves mixture on erythrocytic antioxidant status and gastrointestinal nematodes in kids. Anim. Nutr. Feed Tech. 12: 91-102.
  12. Elzaawely, A.A., Xuan, T.D. and Tawata, S. (2005). Antioxidant and antibacterial activities of Rumex japonicas HOUTT. Aerial Parts Biol. Pharmacol. Bull. 28: 2225-2230.
  13. Gulcin, I., Okyay, M., Kufrayvioglu, O. I. and Asla, A. (2002). Determination of antioxidant activity of Lichen Certraria islandica (L.), acetylcholine. J. Ethnopharmacol. 79: 325-329.
  14. Halliwell, B., Gutteridge, J.M.C. and Aruoma, O.I. (1987). The deoxyribose method: A simple “test-tube” assay for determination of rate constants for reactions of hydroxyl radicals. Anal. Biochem. 165 (1): 215- 219.
  15. Horigome, T., Kumar, R. and Okamotok, K. (1988). Effects of condensed tannins prepared from leaves of fodder plants on digestive enzymes in vitro and in the intestine of rats. Br. J. Nutr. 60: 275-285.
  16. Javanmardi, J., Stushnoff, C., Locke, E. and Vivanco, J. M. (2003). Antioxidant activity and total phenolic content of Iranian Ocimum accessions. Food Chem. 83: 547-550.
  17. Jones, G. A., McAllister, T. A., Muir, A. D. and Cheng, K. J. (1994). Effects of sainfoin (Onobrychis viciifolia Scop.) condensed tannin on growth and proteolysis by four strains of ruminal bacteria. Appl. Envirn. Microbiol. 60: 1374-1378.
  18. Katalinic, V., Milos, M., Kulisic, T. and Jukic, M. (2006). Screening of 70 medicinal plant extracts for antioxidant capacity and total phenols. Food chem. 94: 550-557. 
  19. Makkar, H.P.S. (2000). Quantification of tannins in tree foliage. A laboratory manual for the FAO/IAEA co-ordinated research project. Joint FAO/IAEA working document, IAEA, Viena. pp 1-26.
  20. Makkar, H.P.S. (2003). Effect and fate of tannins in ruminant animals, adaption to tannins and strategies to overcome detrimental effects of feeding tannin-rich feeds. Small Rum. Res. 49: 249–56.
  21. Miller, H.E., Rigelhof, F., Marquart, L., Prakash, A. and Kanter, M. (2000). Antioxidant Content of Whole Grain Breakfast Cereals, Fruits and Vegetables. J. Am. Coll. Nutr. 19(3): 312S-319S.
  22. Min, W., Dalloul, R.A. and Lillehoj, H.S. (2004). Application of biotechnological tools for coccidia vaccine development. J. Vet. Sci. 5: 279-288.
  23. Molan, A. L., Zhuojian, L. I. U. and Shampa, D. E. (2009). Effect of pine bark (Pinus radiata) extracts on sporulation of coccidian oocysts. Folia Parasitol. 56: 1-5.
  24. Nogueira, V. A., France, T. N. and Peixoto, P. V. (2009). Ionophore poisoning in animals. Pesq. Vet. Bras. 29: 191-197.
  25. Ogawa, K., Sakakibara, H., Iwata, R., Ishii, T., Sato, T., Goda, T., Shimoi, K. and Kumazawa, S. (2008). Anthocyanin composition and antioxidant activity of the crowberry (Empetrum nigrum) and other berries. J. Agric. Food Chem. 56: 4457-4462.
  26. Oh, H. I. and Hoff, F. (1986). Effect of condensed grape tannins on the in vitro activity o f digestive proteases and activation of their zymogens. J. Food Sci. 51: 577-580.
  27. Omoruyi, B.E., Bradley, G. and Afolayan, A. (2012). Antioxidant and phytochemical properties of Carpobrotus edulis (L.) bolus leaf used for the management of common infections in HIV/AIDS patients in Eastern Cape Province. BMC Comple. Alter. Med. 12: 215.
  28. Oyaizu, M. (1986). Studies on product of reaction prepared from glucose amine. Jap. J. Nutr. 44: 307-315.
  29. Park, E.J. and Jhon, D.Y. (2010). The antioxidant, angiotensin converting enzyme inhibition activity, and phenolic compounds of bamboo shoot extracts. Food Sci. Tech. 43: 655– 659.
  30. Pathak, A. K. (2013). Potential of using condensed tannins to control gastrointestinal nematodes and improve small ruminant performance. Int. J. Mol. Vet. Res. 3 (8): 36-50. doi: 10.5376/ijmvr.2013.03.0008.
  31. Pathak, A. K., Dutta, N., Banerjee, P. S. and Sharma, K. (2013b). Effect of tannin extracts from tropical tree leaves on larvae and adult Haemonchus contortus. Indian Vet. J. 90 (1): 32-34.
  32. Pathak, A. K., Dutta, N., Banerjee, P. S., Pattanaik, A. K. and Sharma, K. (2013a). Influence of dietary supplementation of condensed tannins through leaf meal mixture on nutrient intake, utilization and performance of Haemonchus contortus infected sheep. Asian-Aust. J. Anim. Sci. 26 (10): 1446-1458.
  33. Pathak, A. K., Dutta, Narayan, Banerjee, P. S., Sharma, K. and Pattanaik, A. K. (2013c). Efficacy of various condensed tannins extracts from tanniferous tree leaves on egg hatching inhibition of Haemonchus contortus. Vet. Pract. 14 (1): 127-129.
  34. Pathak, A. K., Dutta, Narayan., Banerjee, P. S., Goswami, T. K. and Sharma, K. (2014). Effect of condensed tannins supplementation through leaf meal mixture on voluntary feed intake, immune response and worm burden in Haemonchus contortus infected sheep. J. Parasit. Dis. DOI 10.1007/s12639-014-0455-1. Published online: 18 April 2014.
  35. Pathak, A. K., Dutta, Narayan., Pattanaik, A. K., Singh, A., Narang, A. and Sharma, K. (2015). Effect of condensed tannins supplementation from tanniferous tree leaves on methane production and efficiency of microbial biomass production in vitro. Anim. Nutr. Feed Tech. 15 (1):91-100. DOI: 10.5958/0974-181X.2015.00010.4.
  36. Pratt, D. E. (1992). Natural antioxidant from plant material. In: IMT, Huang, CT. HO and CY Lee (Eds). Phenolic compounds in food and their effects on health. N Y Am. Chem. Soc. pp 54-72.
  37. Saradha, M. and Paulsamy, S. (2012). In vitro antioxidant activity and polyphenol estimation of methanolic extract of endangered medicinal tree species, Hildegardia populifolia (Roxb.) Schott & Endl. Int. J. Phytomed. 4: 362-368.
  38. Shah, S. V., Baliga, R., Rajapurkar, M., and Fonseca, V. A. (2007). Oxidants in chronic kidney disease. J. Am. Soc. Nephrol. 18: 16-28.
  39. Shyur, Lie-Fen, Tsung Jieh-hen, Chen Ie-Hsin, Chiu Chih- Yang and Lo Chiu-Ping (2005). A ntioxidant properties of extracts from medicinal plants popularly used in Taiwan, Int J Appl Sci Eng. 3 (3): 195-202.
  40. Singh, Surender, Pathak, A. K., Khan, M. and Sharma, R. K. (2015). Multi- nutrient blocks with a nd without tanniferous leaf meal mixture: Formulation and preparation under sub-tropical environment of Jammu. J. Anim. Res. 5(1): 7-14. DOI Number: 10.5958/2277-940X.2015.00002.9. 
  41. Singleton, V.L., Orthofer, R. and Lamuela-Raventós, R. M. (1999). Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods Enzy. 299: 152-178.
  42. Snedecor, G. W. and Cochran, W. E. (1994). Statistical Methods, 6th Ed. 1967; Oxford and IBH Publishing Co., Delhi, India.
  43. Wei, S.D., Zhou, H.C. and Lin, Y.M. (2010). Antioxidant activities of extract and fractions from the hypocotyls of the mangrove plant Kandelia candel. Int. J. Mol. Sci. 11: 4080-4093.
  44. Zhang, L. L., Lin, Y.M., Zhou, H.C., Wei S. D. and Chen, J. H. (2010). Condensed tannins from mangrove species Kandelia candel and Rhizophora mangle and their antioxidant activity. Mol. 15: 420-431.
  45. Zhang, L.L. and Lin, Y. M. (2008). HPLC, NMR and MALDI-TOF MS analysis of condensed tannins from Lithocarpus glaber leaves with potent free radical scavenging activity. Mol. 13: 2986-2997. 
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