Analysis of genetic diversity of four quails by microsatellite markers

DOI: 10.18805/ijar.8429    | Article Id: B-314 | Page : 1-7
Citation :- Analysis of genetic diversity of four quails by microsatellite markers .Indian Journal Of Animal Research.2016.(50):1-7

Jun.Yan. Bai, You.Zhi. Pang*, Sheng.Jun. Wu, Mei.Qin. Yu and Xiao.Hui. Zhang

pyzh2006@126.com
Address :

College of Animal Science and Technology, 
Henan University of Science and Technology, Henan, Luoyang 471003, China.

Submitted Date : 18-04-2015
Accepted Date : 19-11-2015

Abstract

Genetic diversity of four quail populations, including Korean quails (maroon quails), Peking white quails, Chinese yellow quails and Chinese black quails, were analyzed by microsatellite markers, aiming to provide scientific basis for new breeds of Chinese black quails for egg production as well as the assessment, protection and utilization of Chinese quail’s genetic resources. The results showed that 48 alleles were detected by nine microsatellite markers in the four quail populations, with the mean of 5.33 alleles in each locus. The average effective number of alleles marked by the nine microsatellite markers in Chinese black quails, Peking white quails, Chinese yellow quails and maroon quails were 3.5338, 3.6135, 4.0312 and 3.6508 respectively. The average heterozygosity of the four quail populations were 0.6952, 0.7046, 0.7353 and 0.7096 respectively. The average polymorphic information content of nine microsatellite loci in four quail populations were 0.6204, 0.6587, 0.6942 and 0.6639 , respectively, all of which were greater than 0.5, indicating the four populations’ copious genetic diversity. In this study, the average genetic differentiation coefficient among populations was 0.0349, so the genetic variation among populations accounted for 3.49%, which demonstrated that genetic variation among populations was just a small proportion of the total population genetic variation, and there was little differentiation among the four populations. Cluster analysis indicated that Chinese black quails and Peking white quails were firstly clustered, and then Chinese yellow quails and maroon quails were clustered, and finally the two were clustered together. 

Keywords

Chinese black quails Genetic distance Genetic diversity Microsatellite marker.

References

  1. Amirinia. (2007). Evaluation of Eight Microsatellite Loci Polymorphism in Four Japanese Quail (Coturnix japonica) Strain in Iran. Pakistan Journal of Biological Sciences,10:1195-1199.
  2. Botstein, D., White, R.L., Skolnick, M. and W.Davis, R. (980). Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Am. J. Hum. Genet,32:314-331.
  3. Chang, G.B., Chang, H., Liu, X.P., Wang, H.Y., Xu, W., Zhao, W.M. and Wang, Q.H. (2005). Study on genetic diversity of wild quail in China with microsatellite DNA markers. Acta. Genet. Sin, 8: 795-803.
  4. Farrag,S.A., Tanatarov, A.B., Soltan, M.E., Ismail, M. and Zayed, O.M. (2011). Microsatellite analysis of genetic diversity in three populations of japanese quail (Coturnix coturnix japonica) from kazakhstan. Journal of Animal and Veterinary Advances,18:2376-2383.
  5. Hines. (1981). Linkage relationships among loci of polymorphisms in blood and milk of cattle. OairyIll. Sci, 64:71-76.
  6. Kayan,B.B., Fillon, V., Inoue-Murayama, M., Miwa ., Leroux , S., Fève , K., Monvoisin , J.L., Pitel , F., Vignoles , M., Mouilhayrat, C., Beaumont , C., Ito, S., Minvielle, F., Vignal, A., 2006. Integrated maps in quail (Coturnix japonica) confirm the high degree of synteny conservation with chicken (Gallus gallus) despite 35 million years of divergence. BMC Genomics,7:101.
  7. Meng, Q.M., Sun, Y.Q., Li, D.Q. and Qiao, A.J. (2007). Genetic diversity analysis of Korean quail using microsatellite DNA markers. Fujian J. Anim. Husb. Vet. Med 1:1-2.
  8. Olowofeso, O., Dai, G.J., Wang, J.Y., Xie, K.Z., Li, N.C. and He, Y.Q. (2006). Detection of genetic diversity of four quail populations in East China based on three microsatellite markers. J. Yangzhou University, 1:29-32.
  9. Pang,Y.Z. (2009). Quail Egg from Sexing Supporting Technology Research and Application. China Agriculture Press, Beijing: 62
  10. Wang, Y.H., Chang, H., Xu, W. and Chang, G.B. (2004). Genetic analysis of microsatellite DNA markers in domestic quail and wild Japanese quail populations. Vcta. Veterinariaet. Zootechnica. Sinica, 35:362-366.
  11. Yu.M.Q., Pang, Y.Z., Zhao, S.J., Wu, S.J., Wang, Z.B., Yu, Y.X. and Zhang, X.Y. (2009). Black Feather wood pigeon with quail egg mutant sex-linked genetic mechanisms and its plumage study the relationship between gene interactions. Fifteenth National Animal Genetic Breeding with Symposium Proceedings. China Yangling, 438
  12. Zhao,S.J., Y.Z. Pang., J.Y. Bai. and J.N.Zhang. 2008.Quail egg with yellow feathers karyotype analysis. Eleventh National animal genetic markers with Symposium Proceedings,11:644-647.

Global Footprints