Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

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Indian Journal of Animal Research, volume 51 issue 4 (august 2017) : 660-664

Computer assisted sperm analysis: Relationship between the movement characteristics of buffalo spermatozoa and sire fertility

Raushan K. Singh, A. Kumaresan, M.A. Mir, P. Kumar, S.Chhillar, U.K. Tripathi, S.K. Rajak, S. Nayak, T.K. Mohanty
1<p>Theriogenology Laboratory,&nbsp;ICAR-National Dairy Research Institute, Karnal-132 001, India.</p>
Cite article:- Singh K. Raushan, Kumaresan A., Mir M.A., Kumar P., S.Chhillar, Tripathi U.K., Rajak S.K., Nayak S., Mohanty T.K. (2016). Computer assisted sperm analysis: Relationship between the movement characteristics of buffalo spermatozoa and sire fertility . Indian Journal of Animal Research. 51(4): 660-664. doi: 10.18805/ijar.10768.

The present study was undertaken to identify the differences in sperm kinematics between buffalo bulls with different fertility ratings. Murrah buffalo bulls (n=9) that were routinely used for breeding purpose under progeny testing programme were utilized for the study. Bull fertility was determined based on in vivo fertility trials and the conception rates (CR) were adjusted for different non-genetic parameters. Based on the adjusted CR, bulls were classified into high, medium and low fertile group. Frozen semen samples of these bulls were obtained and sperm kinematic parameters were assessed using a computer assisted sperm analyzer.  The kinematic parameters analyzed included the curvilinear velocity (VCL), the linear velocity (VSL), the average path velocity (VAP), the amplitude of lateral head displacement (ALH), the linearity (LIN), the straightness coefficient (STR) and the beat cross frequency (BCF).  In high fertile bulls, the proportion of motile spermatozoa was higher (p<0.001) than the medium and low fertile bulls. The VAP and VCL of sperm motion were significantly higher (P<0.05) in high fertile bulls compared to either medium or low fertile bulls. The VSL was significantly lower in low fertile bulls (P<0.005) compared to either high or medium fertile bulls. Spermatozoa from high fertile bulls had significantly higher (P<0.05) BCF, STR, ALH and LIN compared to either medium or low fertile bulls. Buffalo bull fertility was significantly and positively correlated with sperm motility, VAP, VSL, VCL and ALH. 

  1. Andrabi, S.M.H. (2009). Factors affecting the quality of cryopreserved buffalo (Bubalus bubalis) bull spermatozoa. Reprod. Domestic Anim. 44: 552-569.

  2. Bailey, J.L., Buhr, M.M. and Robertson, L. (1994). Relationships among in vivo fertility, computer-analysed motility and in vitro Ca2+ flux in bovine spermatozoa. Can. J. Anim. Sci. 74: 53-58.

  3. Barlow, P., Delvigne, A., Van Dromme, J., Van Hoeck, J., Vandenbosch, K. and Leroy, F. (1991). Predictive value of classical and automated sperm analysis for in-vitro fertilization. Hum. Reprod. 6: 1119–1124.

  4. Broekhuijse, M.L.W.J., Sostaric, E., Feitsma, H. and Gadella, B.M. (2012). Relationship of flow cytometric sperm integrity assessments with boar fertility performance under optimized field conditions. J. Anim. Sci. 90: 4327-4336.

  5. Budworth, P.R., Amann, R.P. and Hammerstedt, R.H. (1987). A microcomputer-photographic method for evaluation of motility and velocity of bull sperm. J. Dairy Sci. 70: 1927–1936. 

  6. Dorado, J., Acha, D., Ortiz, I., Galvez, M.J., Carrasco, J.J., Diaz, B. and Hidalgo, M. (2013). Relationship between conventional semen characteristics, sperm motility patterns and fertility of Andalusian donkeys (Equus asinus). Anim. Reprod. Sci. 143: 64-71.

  7. Dorado, J., Munoz-Serrano, A. and Hidalgo, M. (2010). The effect of cryopreservation on goat semen characteristics related to sperm freezability. Anim. Reprod. Sci. 121: 115–123.

  8. Farrell, P.B., Presicce, G.A., Brockett, C.C. and Foote, R.H. (1998). Quantification of bull sperm characteristics measured by computer-assisted sperm analysis (CASA) and the relationship to fertility. Theriogenology. 49: 871-879.

  9. Gadea, J. (2005). Sperm factors related to in vitro and in vivo porcine fertility. Theriogenology. 63: 431–444.

  10. Gillan, L., Kroetsch, T., ChisMaxwell, W.M. and Evans, G. (2008). Assessment of invitro sperm characteristics in relation to fertility in dairy bulls. Anim. Reprod. Sci. 103: 201-214.

  11. Harayama, H., Nishijima, K., Murase, T., Sakase, M. and Fukushima, M. (2010). Relationship of protein tyrosine phosphorylation state withtolerance to frozen storage and the potential to undergo cyclic AMP dependent hyperactivation in the spermatozoa of Japanese black bulls. Mol. Reprod. Dev. 77: 910–921.

  12. Harvey, W.R. (1990). Guide for LSMLMW, PC-1 Version, mixed model least squares and maximum likelihood computer programme. Mimeograph Ohio State Univ., USA.

  13. Hirai, M., Boersma, A., Hoeflich, A., Wolf, E., Foll, J., Aumuller, R. and Braun, J. (2001). Objectively measured sperm motility and sperm head morphometry in boars (Sus scrofa): relation to fertility and seminal plasma growth factors. J. Androl. 22: 104-110.

  14. Holt, C., Holt, W.V., Moore, H.D., Reed, H.C. and Curnock, R.M. (1997). Objectively measured boar sperm motility parameters correlate with the outcomes of on-farm inseminations: results of two fertility trials. J. Androl. 18: 312-323.

  15. Hunter, T. (2000). Signaling—2000 and beyond. Cell. 100: 113-127.

  16. Januskauskas, A., Johannisson, A. and Rodriguez-Martinez, H. (2003). Subtle membrane changes in cryopreserved bull semen in relation with sperm viability, chromatin structure, and field fertility. Theriogenology. 60: 743-758.

  17. Kumar, D., Kumar, P., Singh, P., Yadav, S.P., Sarkar, S.K., Bharadwaj, A. and Yadav, P.S. (2014). Characteristics of frozen thawed semen in predicting the fertility of buffalo bulls. Indian. J. Anim. Sci. 84: 389–392.

  18. Kumaresan, A. and Ansari, M.R. (2001). Evaluation of conception rate in buffaloes (Bubalus bubalis) with reference to semen quality, oestrus stage and inseminator. Indian. J. Anim. Sci. 71: 144-145.

  19. Kumaresan, A., Johannisson, A., Humblot, P. and Bergqvist, A. (2012). Oviductal fluid modulates the dynamics of tyrosine phosphorylation in cryopreserved boar spermatozoa during capacitation. Mol. Reprod. Dev. 79: 525 – 540.

  20. Moore, H.D.M. and Akhondi, M.A. (1996). Fertilizing capacity of rat spermatozoa is correlated with decline in straight-    line velocity measured by continuous computer-aided sperm analysis: epididymal rat spermatozoa from the proximal cauda have a greater fertilizing capacity in vitro than those from the distal cauda or vas deferens. J. Androl. 17: 50–60.

  21. MSP. (2012). Minimum standards for production of bovine frozen semen. National Dairy Development Board, India. pp: 24.

  22. Nie, G.J., Wenzel, J.G.W. and Johnson, K.E. (2002). Comparison of pregnancy outcome in mares among methods used to evaluate and select spermatozoa for insemination. Anim. Reprod. Sci. 69: 211–222.

  23. Olds-Clarke, P. (1996). How does poor motility alter sperm fertilizing ability? J. Androl. 17: 183–186.

  24. Parinaud, J., Richoilley, G., Moutaffian, H., ieitez, G. V. and Mieusset, R. (1996). Are the characteristics of spermatozoa in the insemination medium useful for predicting in vitro fertilization results? Int. J. Androl. 19: 103-108.

  25. Perumal, P., Srivastava, S.K., Ghosh, S.K. and Baruah K.K. (2014). Computer-assisted sperm analysis of freezable and nonfreezable Mithun (Bos frontalis) semen. Journal of Animals. 18: 2014.

  26. Perumal, P., Selvaraju, S., Selvakumar, S., Barik, A.K., Mohanty, D.N., Das, S., Das, R.K. and Mishra, P.C. (2011). Effect of pre-freeze addition of cysteine hydrochloride and reduced glutathione in semen of crossbred jersey bulls on sperm parameters and conception rates. Reprod. Domestic Anim. 46: 636–641.

  27. Rodriguez-Martinez, H. (2013). Semen evaluation techniques and their relationship with fertility. Anim. Reprod. 10: 148-159.

  28. Sanchez-Partida, L.G., Windsor, D.P., Eppleston, J., Setchell, B.P. and Maxwell, W.M.C. (1999). ertility and its relationship to motility characteristics of spermatozoa in ewes after cervical, transcervical and intrauterine insemination with frozen-thawed ram semen. J. Androl. 20: 280–288.

  29. Silva, A.R., Cardoso, R.D.C.S., Silva, L.D.M., Chirinea, V.H., Lopes, M.D. and Souza, F.F. (2006). Prognostic value of canine frozen-thawed semen parameters on in vitro sperm-oocyte interactions. Theriogenology. 66: 456–462.

  30. Srivastava, A.K. and Kumaresan, A. (2014). Scope of buffaloes in Indian dairy industry. Asian Buffalo Magazine. pp: 16-27. 

  31. Tanghe, S., Van Soom, A., Sterckx, V., Maes, D. and De Kruif, A. (2002). Assessment of different sperm quality parameters to predict in vitro fertility of bulls. Reprod. Domestic Anim. 37: 127-132.

  32. Van den Bergh, M., Emiliani, S., Biramane, J., Vannin, A.S. and Englert, Y. (1998). A first prospective study of the individual straight line velocity of the spermatozoon and its influences on the fertilization rate after intra-cytoplasmic sperm injection. Hum. Reprod. 13: 3103–3107.

  33. Verstegen, J., Iguer-Ouada, M. and Onclin, K. (2002). Computer assisted semen analyzers in andrology research and veterinary practice. Theriogenology. 57: 149–179.

  34. Watson, P.F. (2000). The causes of reduced fertility with cryopreserved semen. Anim. Reprod. Sci. 60: 481-492.

  35. Zhang, B.R., Larsson, B., Lundeheim, N. and RodriguezMartinez, H. (1998). Sperm characteristics and zona pellucida binding in relation to field fertility of frozen thawed semen from dairy AI bulls. Int. J. Androl. 21: 207-216.


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