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Current IssueEditorial BoardOnline First ArticlesArchive

Research Article

volume 51 issue 6 (december 2017) : 993-997,   Doi: 10.18805/ijar.v0iOF.9136

Assessment of antioxidants for preservation of crossbred bull semen in Tris based extender

T
T.K.S. Rao
T
T.K. Mohanty
M
M. Bhakat
1Artificial Breeding Research Center, National Dairy Research Institute, Karnal-132 001, Haryana, India
Cite article:- Rao T.K.S., Mohanty T.K., Bhakat M. (2017). Assessment of antioxidants for preservation of crossbred bull semen in Tris based extender. Indian Journal of Animal Research. 51(6): 993-997. doi: 10.18805/ijar.v0iOF.9136.

ABSTRACT

Present study was aimed to improve poor quality crossbred bulls semen, as occurrence of poor quality ejaculates are one of the major problems in crossbred bulls. Antioxidants and its combination was tried to overcome such problems. Vitamin E, Vitamin C and Vitamin E+C were supplemented at dose rate of 1mg, 5 mM and 1mg + 5mM per ml respectively in split ejaculates to evaluate semen quality during preservation at refrigerated temperature and cryopreservation. The results showed that semen characteristics were better in antioxidant supplemented ejaculates. Total sperm abnormality were significantly lower (p<0.05) in antioxidant supplemented group.  The seminal characteristics of crossbred bull semen showed significantly better (p<0.05) performance on preservation, when fortified with Vitamin E as compared to Vitamin C alone as well as Vitamin E and C in combination. The performance of semen additives, were supportive to semen characteristics especially for Vitamin E, moreover fortification with Vitamin E+C was slightly superior to Vitamin C. It can be concluded that fortification of Vitamin E has beneficial role in semen quality improvement followed by Vitamin E+C and Vitamin C. 

REFERENCES

  1. Akhter, S., Rakha, B. A., Ansari, M. S., Andrabi, S. M. H. and Ullah, N. (2011). Storage of Nili-Ravi buffalo (Bubalus bubalis) semen in skim milk extender supplemented with ascorbic acid and á-tocopherol. Pakistan J. Zool., 43 (2): 273-277. 
  2. Andrabi, S. M. H. (2009). Factors affecting the quality of cryopreserved buffalo (Bubalus bubalis) bull spermatozoa. Reprod. Dom. Anim., 44: 552-569.
  3. Andrabi, S. M. H., Ansari, M. S., Ullah, N. and Afzal, M. (2008). Effect of non-enzymatic antioxidants in extender on post-thaw quality of buffalo (Bubalus bubalis) bull spermatozoa. Pakistan Vet. J., 28: 159-162.
  4. Anghel, A., Zamfirescu, S., Coprean, D., Sogorescu, Elena. (2000).The effects of cystein, bovine serum albumin and vitamin e on the calitative parameters of frozen thawed ram semen. Annals of RSCB. Vol. XIV, Issue 2.
  5. Azawi, O. I. and Hussein, E. K. (2013). Effect of Vitamin C or E supplementation to Tris diluent on semen quality of Awassi rams preserved at 5oC. Vet Res Forum, 4 (3): 157-160.
  6. Balla, B. A., Medinab, V., Gravancea C. G. and Baumbera, J. (2001). Effect of antioxidants on preservation of motility, viability and acrosomal integrity of equine spermatozoa during storage at 5°C. Theriogenology, 56(4): 577-589. 
  7. Bansal, A. K. and Bilaspuri, G. S. (2010). Impact of oxidative stress and antioxidants on semen functions. Vet. Med. Int. 2010: 686137.
  8. Batool, A., Mehboob, Kh, Quadeer, S., Sajjad Ansari, M., Allah Rakha, B., Ullah, N., Hassan Andrabi, S. M. and Akhter, Sh. (2012). Pakistan J. Zool, 44 (6), 1487-1491.
  9. Baumber, J., Ball, B.A., Gravance, C.G., Medina, V. and Davies-morel, M.C.G. (2000). The effect of reactive oxygen species on equine sperm motility, viability, acrosome integrity, mitochondrial membrane potential and membrane lipid peroxidation. J. Androl. 21:895-902.
  10. Beheshti, R., Asadi, A., Maheri-Sis, N. (2011). The effect of vitamin E on post-thawed buffalo bull sperm parameters. J. Am. Sci. 7(6): 227-231.
  11. Bhakat, M., T.K. Mohanty, V.S. Raina, A.K. Gupta, P.K. Pankaj, R.K. Mahapatra and M. Sarkar. (2011). Study on suitable semen additives incorporation into the extender stored at refrigerated temperature. Asian Australas. J. Anim. Sci, 24(10): 1348-1357.
  12. Blesbois, E., Grasseau, J. and Seigneurin, F. (2005). Membrane fluidity and the ability of domestic bird spermatozoa to survive cryopreservation. Reproduction, 129: 371-378.
  13. Boonsorn, T., Kongbuntad, W., Narkkong, N. A. (2010). Effect of catechin addition to extender on sperm quality and lipid peroxidation in boar semen. Am. Eurasian J. Agric. Environ. Sci., 7:283-288.
  14. Breininger, E., Beorlegui, B., Cristian, M. and Beconi, T. (2005). Alpha-tocopherol improves biochemical and dynamic parameters in cryopreserved boar semen. Theriogenology, 63: 2126-2135.
  15. Buetter, G.R. (1993). The pecking order of free radicals and oxidants: lipid peroxidation, alpha-tocopherol and ascorbate. Arch Biochem. Biophys., 300: 535-543. 
  16. Cerolini, S., Maldjian, A., Surai, P. and Nobel, R. (2000). Viability and susceptibility to lipid peroxidation to lipid peroxidation and fatty acid composition of boar semen during liquid storage. Anim. Reprod. Sci., 58: 99-11.
  17. Chaudhari, D. V., Dhami, A. J., Hadiya, K. K. and Patel, J. A. (2015). Relative efficacy of egg yolk and soya milk based extenders for cryopreservation (-196oC) of buffalo semen. Vet. World., 8(2): 239-244.
  18. Fraga, C. D., Motchnik, P. A., Shinegana, M.K. Helbock, H.J., Jacob, R. A., Ames, B. N. (1991). Ascorbic acid protects aginst oxidative DNA damage in human sperm. Proc. Natl. Acad. Sci., 88:1103-1106. 
  19. Gadea, J., Selles, E., Macro, M. A. and Coy, P. Matás C, Romar R and Ruiz S. (2004). Decrease in glutathione content in boar sperm after cryopreservation. Effect of the addition of reduced glutathione to the freezing and thawing extenders. Theriogenology, 62: 690-701.
  20. Halliwell, B., Gutteridge, J. M. C. (1999). Free Radicals in Biology and Medicine. 3rd ed.: Oxford University Press, New York, USA, 122-167.
  21. Hartono, M. (2008). Optimalization of Vitamin E in egg yolk citrate extender to preserve semen quality of “Boer” goat. Indonesian Trop. Anim. Agric., 33 (1): 11-19. ISSN 0410-6320
  22. Herdis, I., Kusuma, M., Surachman, M., Riza, I. K., Sutama, I., Inounu, B. P. and Arifiantini, I. (2002). Improvement of frozen semen quality of Garut Sheep through the addition of alpha-tocopherol into yolk egg-skim milk diluents. Jurnal Ilmu Ternak dan Veteriner 7 (1): 12-17.
  23. Jeyendran, R.S., van der Ven, H.H., Perez Pelaez, M., Crabo, B.G., Zaneveld, L.J.D. (1984). Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characteristics. J. Reprod. Fertil., 70:219-28.
  24. Kurien, V. (1987). True dimension of Dairy development- In Dairy India pp. 21, 3rd ed (P. R. Gupta, ed.) Priyadershin Vihar, Delhi, India. 
  25. Lewis, S. E. M., Sterling, E. S. L., Young, I. S. (1997). Comparison of individual antioxidants of sperm and seminal plasma in fertile and infertile men. Fertil. Steril., 67:142-147.
  26. Luvoni, G. C. (2006). Gamete cryopreservation in the domestic cat. Theriogenology, 66 (1):101-111.
  27. Mittal, P. K., Ananad, M., Madan, A. K., Yadav, S. and Kumar, J. (2014). Antioxidant capacity of Vitamin E, Vitamin C and their combination in cryopreserved Bhadawari bull semen. Vet. World., 7:1127-1131.
  28. Michael, A. J., Alexopoulos, C., Pontiki, E. A., Hadjipavlou-Litina, D. J., Saratsis, Ph., Ververidis, H. N., Boscos, C. M. (2007). Quality and reactive oxygen species of extended canine semen after vitamin C supplementation. Theriogenology, 70:827-835.
  29. Niki, E. (1991). Action of Ascorbic acid as a scavenger of active and stable oxygen radicals. Am. J. Clin. Nutr., 54: 1119s-1124s.
  30. Pena, A. I., Lugilde, L. L., Barrio, M., Herradon, P. G. and Quintela, L. A. (2003). Effect of Equex from different sources on post thaw survival, longevity and intracellular Ca2+ concentration of dog spermatozoa. Theriogenology, 59 (8): 1725-1739.
  31. Pour, H. A., Tashmasbi, A. and Naserain, A. (2013). The influence of Vitamin E on semen characteristics of ghezel rams in during cooling and frozen process. Euro. J. Zool. Res., 2 (5): 94-99. 
  32. Sajjad, M., Ali, S., Ullah, N., Anwar, M., Akhter, S. and Andrabi, S. M. H. (2007). Blood serum testosterone level and its relationship with scrotal circumference and semen characteristics in Nili-Ravi buffalo bulls. Pakistan Vet. J., 27: 63-66. 
  33. Saleh, R and Agarwal, A. (2002). Saleh, R.A. and Agarwal, A. (2002) Oxidative stress and male infertility: From research bench to clinical practice. J. Androl, 23: 737-752. 
  34. Shukla, M.S. and Misra, A.K. (2005). Effect of antioxidants alpha -tocopherol, ascorbic acid and n-propyl gallate on Murrah semen cryopreservation. Buffalo J., 21(1):27-38.
  35. Sies, H. (1993). Stratgies of antioxidant defense. Eur.J.Biochem. 215:213-219.
  36. Singh, C. V. (2016). Crossbreeding in cattle for milk production: achievements, challenges and opportunities in India- A review. Adv.Dairy Res., 4 (3): 158. 
  37. Snedecor, G.W. and Cochran, W.G. (1994). Statistical Methods. 6th edn., Oxford and IBH publ. co., New Delhi. 
  38. Sonmez, M. and Demirci, E. (2004). The effect of ascorbic acid on the freezability of ram semen dilutd with extenders containing different proportion of glycerol. Turk. J. Ve.t Anim. Sci. 28: 893-899.
  39. Storey, B.T. (1997). Biochemistry of the induction and prevention of lipoperoxidative damage in human spermatozoa. Mol. Hum.Reprod. 3:203-213.
  40. Stradaioli, G., Noro, T., Sylla, L. and Monaci, M. (2007). Decrease in glutathione (GSH) content in bovine sperm after cryopreservation: Comparison between two extenders. Theriogenology, 67: 1249-1255.
  41. Swain, D.K. and Kundu, A. K. (2009). Effect of antioxidants on membrane integrity of cryopreserved bull sperm. Indian Vet. J., 86:1076-1077.
  42. Tabatabaei, S., Batavani, R. and Ayen, E. (2011). Effect of Vitamin E addition to chicken semen on sperm quality during in vitro storage of semen. Vet. Res. Forum, 2 (2): 103-111. 
  43. Verma, A. and Kanwar, K.C. (1998). Human sperm motility and lipid peroxidation in different ascorbic acid concentrations: An invitro analysis. Androlgia, 30:325-329. 
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Indian Journal of Animal Research
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    Research Article

    volume 51 issue 6 (december 2017) : 993-997,   Doi: 10.18805/ijar.v0iOF.9136

    Assessment of antioxidants for preservation of crossbred bull semen in Tris based extender

    T
    T.K.S. Rao
    T
    T.K. Mohanty
    M
    M. Bhakat
    1Artificial Breeding Research Center, National Dairy Research Institute, Karnal-132 001, Haryana, India
    Cite article:- Rao T.K.S., Mohanty T.K., Bhakat M. (2017). Assessment of antioxidants for preservation of crossbred bull semen in Tris based extender. Indian Journal of Animal Research. 51(6): 993-997. doi: 10.18805/ijar.v0iOF.9136.

    ABSTRACT

    Present study was aimed to improve poor quality crossbred bulls semen, as occurrence of poor quality ejaculates are one of the major problems in crossbred bulls. Antioxidants and its combination was tried to overcome such problems. Vitamin E, Vitamin C and Vitamin E+C were supplemented at dose rate of 1mg, 5 mM and 1mg + 5mM per ml respectively in split ejaculates to evaluate semen quality during preservation at refrigerated temperature and cryopreservation. The results showed that semen characteristics were better in antioxidant supplemented ejaculates. Total sperm abnormality were significantly lower (p<0.05) in antioxidant supplemented group.  The seminal characteristics of crossbred bull semen showed significantly better (p<0.05) performance on preservation, when fortified with Vitamin E as compared to Vitamin C alone as well as Vitamin E and C in combination. The performance of semen additives, were supportive to semen characteristics especially for Vitamin E, moreover fortification with Vitamin E+C was slightly superior to Vitamin C. It can be concluded that fortification of Vitamin E has beneficial role in semen quality improvement followed by Vitamin E+C and Vitamin C. 

    REFERENCES

    1. Akhter, S., Rakha, B. A., Ansari, M. S., Andrabi, S. M. H. and Ullah, N. (2011). Storage of Nili-Ravi buffalo (Bubalus bubalis) semen in skim milk extender supplemented with ascorbic acid and á-tocopherol. Pakistan J. Zool., 43 (2): 273-277. 
    2. Andrabi, S. M. H. (2009). Factors affecting the quality of cryopreserved buffalo (Bubalus bubalis) bull spermatozoa. Reprod. Dom. Anim., 44: 552-569.
    3. Andrabi, S. M. H., Ansari, M. S., Ullah, N. and Afzal, M. (2008). Effect of non-enzymatic antioxidants in extender on post-thaw quality of buffalo (Bubalus bubalis) bull spermatozoa. Pakistan Vet. J., 28: 159-162.
    4. Anghel, A., Zamfirescu, S., Coprean, D., Sogorescu, Elena. (2000).The effects of cystein, bovine serum albumin and vitamin e on the calitative parameters of frozen thawed ram semen. Annals of RSCB. Vol. XIV, Issue 2.
    5. Azawi, O. I. and Hussein, E. K. (2013). Effect of Vitamin C or E supplementation to Tris diluent on semen quality of Awassi rams preserved at 5oC. Vet Res Forum, 4 (3): 157-160.
    6. Balla, B. A., Medinab, V., Gravancea C. G. and Baumbera, J. (2001). Effect of antioxidants on preservation of motility, viability and acrosomal integrity of equine spermatozoa during storage at 5°C. Theriogenology, 56(4): 577-589. 
    7. Bansal, A. K. and Bilaspuri, G. S. (2010). Impact of oxidative stress and antioxidants on semen functions. Vet. Med. Int. 2010: 686137.
    8. Batool, A., Mehboob, Kh, Quadeer, S., Sajjad Ansari, M., Allah Rakha, B., Ullah, N., Hassan Andrabi, S. M. and Akhter, Sh. (2012). Pakistan J. Zool, 44 (6), 1487-1491.
    9. Baumber, J., Ball, B.A., Gravance, C.G., Medina, V. and Davies-morel, M.C.G. (2000). The effect of reactive oxygen species on equine sperm motility, viability, acrosome integrity, mitochondrial membrane potential and membrane lipid peroxidation. J. Androl. 21:895-902.
    10. Beheshti, R., Asadi, A., Maheri-Sis, N. (2011). The effect of vitamin E on post-thawed buffalo bull sperm parameters. J. Am. Sci. 7(6): 227-231.
    11. Bhakat, M., T.K. Mohanty, V.S. Raina, A.K. Gupta, P.K. Pankaj, R.K. Mahapatra and M. Sarkar. (2011). Study on suitable semen additives incorporation into the extender stored at refrigerated temperature. Asian Australas. J. Anim. Sci, 24(10): 1348-1357.
    12. Blesbois, E., Grasseau, J. and Seigneurin, F. (2005). Membrane fluidity and the ability of domestic bird spermatozoa to survive cryopreservation. Reproduction, 129: 371-378.
    13. Boonsorn, T., Kongbuntad, W., Narkkong, N. A. (2010). Effect of catechin addition to extender on sperm quality and lipid peroxidation in boar semen. Am. Eurasian J. Agric. Environ. Sci., 7:283-288.
    14. Breininger, E., Beorlegui, B., Cristian, M. and Beconi, T. (2005). Alpha-tocopherol improves biochemical and dynamic parameters in cryopreserved boar semen. Theriogenology, 63: 2126-2135.
    15. Buetter, G.R. (1993). The pecking order of free radicals and oxidants: lipid peroxidation, alpha-tocopherol and ascorbate. Arch Biochem. Biophys., 300: 535-543. 
    16. Cerolini, S., Maldjian, A., Surai, P. and Nobel, R. (2000). Viability and susceptibility to lipid peroxidation to lipid peroxidation and fatty acid composition of boar semen during liquid storage. Anim. Reprod. Sci., 58: 99-11.
    17. Chaudhari, D. V., Dhami, A. J., Hadiya, K. K. and Patel, J. A. (2015). Relative efficacy of egg yolk and soya milk based extenders for cryopreservation (-196oC) of buffalo semen. Vet. World., 8(2): 239-244.
    18. Fraga, C. D., Motchnik, P. A., Shinegana, M.K. Helbock, H.J., Jacob, R. A., Ames, B. N. (1991). Ascorbic acid protects aginst oxidative DNA damage in human sperm. Proc. Natl. Acad. Sci., 88:1103-1106. 
    19. Gadea, J., Selles, E., Macro, M. A. and Coy, P. Matás C, Romar R and Ruiz S. (2004). Decrease in glutathione content in boar sperm after cryopreservation. Effect of the addition of reduced glutathione to the freezing and thawing extenders. Theriogenology, 62: 690-701.
    20. Halliwell, B., Gutteridge, J. M. C. (1999). Free Radicals in Biology and Medicine. 3rd ed.: Oxford University Press, New York, USA, 122-167.
    21. Hartono, M. (2008). Optimalization of Vitamin E in egg yolk citrate extender to preserve semen quality of “Boer” goat. Indonesian Trop. Anim. Agric., 33 (1): 11-19. ISSN 0410-6320
    22. Herdis, I., Kusuma, M., Surachman, M., Riza, I. K., Sutama, I., Inounu, B. P. and Arifiantini, I. (2002). Improvement of frozen semen quality of Garut Sheep through the addition of alpha-tocopherol into yolk egg-skim milk diluents. Jurnal Ilmu Ternak dan Veteriner 7 (1): 12-17.
    23. Jeyendran, R.S., van der Ven, H.H., Perez Pelaez, M., Crabo, B.G., Zaneveld, L.J.D. (1984). Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characteristics. J. Reprod. Fertil., 70:219-28.
    24. Kurien, V. (1987). True dimension of Dairy development- In Dairy India pp. 21, 3rd ed (P. R. Gupta, ed.) Priyadershin Vihar, Delhi, India. 
    25. Lewis, S. E. M., Sterling, E. S. L., Young, I. S. (1997). Comparison of individual antioxidants of sperm and seminal plasma in fertile and infertile men. Fertil. Steril., 67:142-147.
    26. Luvoni, G. C. (2006). Gamete cryopreservation in the domestic cat. Theriogenology, 66 (1):101-111.
    27. Mittal, P. K., Ananad, M., Madan, A. K., Yadav, S. and Kumar, J. (2014). Antioxidant capacity of Vitamin E, Vitamin C and their combination in cryopreserved Bhadawari bull semen. Vet. World., 7:1127-1131.
    28. Michael, A. J., Alexopoulos, C., Pontiki, E. A., Hadjipavlou-Litina, D. J., Saratsis, Ph., Ververidis, H. N., Boscos, C. M. (2007). Quality and reactive oxygen species of extended canine semen after vitamin C supplementation. Theriogenology, 70:827-835.
    29. Niki, E. (1991). Action of Ascorbic acid as a scavenger of active and stable oxygen radicals. Am. J. Clin. Nutr., 54: 1119s-1124s.
    30. Pena, A. I., Lugilde, L. L., Barrio, M., Herradon, P. G. and Quintela, L. A. (2003). Effect of Equex from different sources on post thaw survival, longevity and intracellular Ca2+ concentration of dog spermatozoa. Theriogenology, 59 (8): 1725-1739.
    31. Pour, H. A., Tashmasbi, A. and Naserain, A. (2013). The influence of Vitamin E on semen characteristics of ghezel rams in during cooling and frozen process. Euro. J. Zool. Res., 2 (5): 94-99. 
    32. Sajjad, M., Ali, S., Ullah, N., Anwar, M., Akhter, S. and Andrabi, S. M. H. (2007). Blood serum testosterone level and its relationship with scrotal circumference and semen characteristics in Nili-Ravi buffalo bulls. Pakistan Vet. J., 27: 63-66. 
    33. Saleh, R and Agarwal, A. (2002). Saleh, R.A. and Agarwal, A. (2002) Oxidative stress and male infertility: From research bench to clinical practice. J. Androl, 23: 737-752. 
    34. Shukla, M.S. and Misra, A.K. (2005). Effect of antioxidants alpha -tocopherol, ascorbic acid and n-propyl gallate on Murrah semen cryopreservation. Buffalo J., 21(1):27-38.
    35. Sies, H. (1993). Stratgies of antioxidant defense. Eur.J.Biochem. 215:213-219.
    36. Singh, C. V. (2016). Crossbreeding in cattle for milk production: achievements, challenges and opportunities in India- A review. Adv.Dairy Res., 4 (3): 158. 
    37. Snedecor, G.W. and Cochran, W.G. (1994). Statistical Methods. 6th edn., Oxford and IBH publ. co., New Delhi. 
    38. Sonmez, M. and Demirci, E. (2004). The effect of ascorbic acid on the freezability of ram semen dilutd with extenders containing different proportion of glycerol. Turk. J. Ve.t Anim. Sci. 28: 893-899.
    39. Storey, B.T. (1997). Biochemistry of the induction and prevention of lipoperoxidative damage in human spermatozoa. Mol. Hum.Reprod. 3:203-213.
    40. Stradaioli, G., Noro, T., Sylla, L. and Monaci, M. (2007). Decrease in glutathione (GSH) content in bovine sperm after cryopreservation: Comparison between two extenders. Theriogenology, 67: 1249-1255.
    41. Swain, D.K. and Kundu, A. K. (2009). Effect of antioxidants on membrane integrity of cryopreserved bull sperm. Indian Vet. J., 86:1076-1077.
    42. Tabatabaei, S., Batavani, R. and Ayen, E. (2011). Effect of Vitamin E addition to chicken semen on sperm quality during in vitro storage of semen. Vet. Res. Forum, 2 (2): 103-111. 
    43. Verma, A. and Kanwar, K.C. (1998). Human sperm motility and lipid peroxidation in different ascorbic acid concentrations: An invitro analysis. Androlgia, 30:325-329. 
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