Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

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Indian Journal of Animal Research, volume 51 issue 4 (august 2017) : 648-653

Detection of osteopontin transcript in seminal plasma and its association with post-freeze-thaw quality of cryopreserved spermatozoa in mithun (Bos frontalis)

K.K. Baruah, A. Dhali, B. Bora, A. Mech, M. Mondal
1<p>ICAR-National Research Centre on Mithun,&nbsp;Jharnapani, Medziphema - 797 106, Nagaland, India.</p>
Cite article:- Baruah K.K., Dhali A., Bora B., Mech A., Mondal M. (2016). Detection of osteopontin transcript in seminal plasma and its association with post-freeze-thaw quality of cryopreserved spermatozoa in mithun (Bos frontalis) . Indian Journal of Animal Research. 51(4): 648-653. doi: 10.18805/ijar.v0iOF.4553.

The study aimed to detect osteopontin (OPN) transcript in seminal plasma and to assess the relationship between the presence of the transcript and post-freeze-thaw quality of cryopreserved spermatozoa in mithun (Bos frontalis). Semen samples were collected from five adult bulls through rectal massage method and cryopreserved in tris-egg yolk-glycerol extender. OPN transcript was detected by RT-PCR in the RNA purified from seminal plasma. OPN transcript was found to be present consistently in three animals (OPN+) and absent in two animals (OPN-). Although, sperm viability and acrosomal integrity were found similar at different stages of cryopreservation in both the groups, tail abnormality after final dilution and, head and tail abnormalities after equilibration were found significantly (P<0.05) lesser in the OPN+ compared to OPN- animals. The results indicated that OPN is probably important in stabilizing sperm membrane that resulted in better resistance of sperm to cryopreservation process in OPN+ animals. 

  1. Almeida, E.A., Huovila, A.P., Sutherland, A.E., Stephens, L.E., Calarco, P.G., Shaw, L.M., et al. (1995). Mouse egg integrin alpha 6 beta 1 functions as a sperm receptor. Cell, 81: 1095–1104.

  2. Baruah, K.K., Dhali, A., Mech, A., Bora, B., Das, J., Bora, R., et al. (2013). Effect of concentration and addition method of glycerol on the quality of cryopreserved mithun (Bos frontalis) spermatozoa. J. Anim. Physiol. Anim. Nutr., 97: 1051-1058. 

  3. Boccia, L., Francesco, S.D., Neglia, G., Blasi, M.D., Longobardi, V., Campanile, G. et al. (2013). Osteopontin improves sperm capacitation and in vitro fertilization efficiency in buffalo (Bubalus bubalis). Theriogenology, 80: 212–217.

  4. Brown, L.F., Berse, B., Van de Water, L., Papadopoulos-Sergiou, A., Perruzzi, C.A., Manseau, E.J., et al. (1992). Expression and distribution of osteopontin in human tissues: wide spread association with luminal epithelial surfaces. Mol. Biol. Cell, 3: 1169–1180.

  5. Cancel, A.M., Chapman, D.A. and Killian, G.J. (1997). Osteopontin is the 55-kilodalton fertility-associated protein in holstein bull seminal plasma. Biol. Reprod., 57: 1293-1301. 

  6. Cancel, A.M., Chapman, D.A. and Killian, G.J. (1999). Osteopontin localization in the holstein bull reproductive tract. Biol. Reprod., 60: 454–460.

  7. Craig, A.M. and Denhardt, D.T. (1991). The murine gene encoding secreted phosphoprotein 1 (osteopontin): promoter structure, activity, and induction in vivo by estrogen and progesterone. Gene, 100: 163–171. 

  8. Denhardt, D.T. and Guo, X. (1993). Osteopontin: a protein with diverse functions. FASEB J., 7: 1475–1482.

  9. Desnoyers, L. and Manjunath, P. (1992). Major proteins of bovine seminal plasma exhibit novel interactions with phospholipid. J. Biol. Chem., 267: 10149–10155.

  10. Dhali, A., Karunakaran, M., Mech, A., Nath, N., Prakash, B., Rajkhowa, C. and Mishra, D.P. (2008). Birth of the first mithun (Bos frontalis) calf through artificial insemination. Animal, 2: 879-882. 

  11. Erikson, D.W., Way, A.L., Chapman, D.A. and Killian, G.J. (2007). Detection of osteopontin on Holstein bull spermatozoa, in cauda epididymal fluid and testis homogenates, and its potential role in bovine fertilization. Reproduction, 133: 909–917.

  12. Fraser, L.R., Das Gupta, S. and Mills, C.L. (1996). Fertilization-calcium-dependent events. Fertil. Steril., 65: 1258–1259.

  13. Gabler, C., Chapman, D.A. and Killian, G.J. (2003). Expression and presence of osteopontin and integrins in the bovine oviduct during the oestrous cycle. Reproduction, 126: 721–729.

  14. Goncalves, R.F., Chapman, D.A., Bertolla, R.P., Eder, I. and Killian, G.J. (2008). Pre-treatment of cattle semen or oocytes with purified milk osteopontin affects in vitro fertilization and embryo development. Anim. Reprod. Sci., 108: 375–383.

  15. Killian, G.J., Chapman, D.A. and Rogowski, L.A. (1993). Fertility-associated proteins in Holstein bull seminal plasma. Biol. Reprod., 49: 1202–1207.

  16. Kovacs, A. and Foote, R.H. (1992). Viability and acrosome staining of bull, boar and rabbit spermatozoa. Biotech. Histochem., 67:119-124

  17. Luedtke, C.C., McKee, M.D., Cyr, D.G., Gregory, M., Kaartinen, M.T., Mui., J., et al. (2002). Osteopontin expression and regulation in the testis, efferent ducts and epididymis of rats during postnatal development through to adulthood. Biol. Reprod., 66: 1437–1448.

  18. Mahmoud, K.G.M., El-Sokary, A.A.E., Abou El-Roos, M.E.A., Abdel Ghaffar, A.D. and Nawito, M. (2013). Sperm characteristics in cryopreserved buffalo bull semen and field fertility. Iranian J. Appl. Anim. Sci., 3: 777-783. 

  19. Manjunath, P. and Therien, I. (2002). Role of seminal plasma phospholipid-binding proteins in sperm membrane lipid modification that occurs during capacitation. J. Reprod. Immunol., 53: 109–119.

  20. Metz, K.W., Berger, T. and Clegg, E.D. (1990). Adsorption of seminalplasma-proteins by boar spermatozoa. Theriogenology, 34: 691–700.

  21. Monaco, E., Gasparrini, B., Boccia, L., Rosa, A.D., Attanasio, L., Zicarelli, L. and Killian, G. (2009). Effect of osteopontin (OPN) on in vitro embryo development in cattle. Theriogenology, 71: 450–457.

  22. Moura, A.A., Chapman, D.A. and Killian, G.J. (2006). Proteins of the accessory sex glands associated with the oocyte-    penetrating capacity of cauda epididymal sperm from holstein bulls of documented fertility. Mol. Reprod. Dev., 74: 214–222.

  23. Muino-Blanco, T., Perez-Pe, R. and Cebrian-Perez, J.A. (2008). Seminal Plasma Proteins and Sperm Resistance to Stress. Reprod. Domest. Anim., 43: 18–31. 

  24. Ollero, M., Pascual, M.L., Muino-Blanco, T., Cebrian-Perez, J.A. and Lopez-Perez, M.J. (1994). Revealing surface changes associated with maturation of ram spermatozoa by centrifugal counter-current distribution in an aqueous two-    phase system. J. Chromatogr. A., 668: 173–178.

  25. Pero, M.E., Killian, G.J., Lombardi, P., Zicarelli, L., Avallone, L. and Gasparrini, B. (2006). Identification of osteopontin in water buffalo semen. Reprod. Fertil. Dev., 19: 279.

  26. Rebolledo, A.D., Sierra, L.N., Tamayo, A.C., Loria, A.A., Denis, S.E., Oses, R.B., et al., (2007). Fertility in hair sheep inseminated with freeze spermatozoa rediluted with seminal plasma. Rev. Cient. Fac. Cienc. Veter., 17: 73–76.

  27. Rodriguez, C.M., Day, J.R. and Killian, G.J. (2000). Osteopontin gene expression in the holstein bull reproductive tract. J. Androl., 21: 414–420. 

  28. Siiteri, J.E., Ensrud, K.M., Moore, A. and Hamilton, D.W. (1995). Identification of osteopontin (OPN) mRNA and protein in the rat testis and epididymis, and on sperm. Mol. Reprod. Dev., 40: 16–28.

  29. Souza, C.E.A., Moura, A.A., Monaco, E. and Killian, G.J. (2008). Binding patterns of bovine seminal plasma proteins A1D A2, 30 kDa and osteopontin on ejaculated sperm before and after incubation with isthmic and ampullary oviductal fluid. Anim. Reprod. Sci., 105: 72–89.

  30. Wolf, D. and Voglmayr, J. (1984). Diffusion and regionalization in membranes of maturing ram spermatozoa. J. Cell. Boil., 98: 1678–1684.


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