Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

  • Print ISSN 0367-6722

  • Online ISSN 0976-0555

  • NAAS Rating 6.50

  • SJR 0.263

  • Impact Factor 0.4 (2024)

Frequency :
Monthly (January, February, March, April, May, June, July, August, September, October, November and December)
Indexing Services :
Science Citation Index Expanded, BIOSIS Preview, ISI Citation Index, Biological Abstracts, Scopus, AGRICOLA, Google Scholar, CrossRef, CAB Abstracting Journals, Chemical Abstracts, Indian Science Abstracts, EBSCO Indexing Services, Index Copernicus
Submit

Research Article

Indian Journal of Animal Research, volume 51 issue 4 (august 2017) : 732-736

Molecular detection of porcine circo virus type 2 and porcine parvo virus in pigs having reproductive problems and histopathological studies in the tissue of aborted pig foetuses 

S.R. Pegu, D.K. Sarma, S. Rajkhowa, M. Choudhury, D. Sarma, J.P. Das
1<p>ICAR-National Research Centre on Pig,&nbsp;Guwahati-781 131, Assam, India.</p>
Cite article:- Pegu S.R., Sarma D.K., Rajkhowa S., Choudhury M., Sarma D., Das J.P. (2016). Molecular detection of porcine circo virus type 2 and porcine parvo virus inpigs having reproductive problems and histopathologicalstudies in the tissue of aborted pig foetuses . Indian Journal of Animal Research. 51(4): 732-736. doi: 10.18805/ijar.v0i0f.3790.

ABSTRACT

The present study was undertaken to detect the presence of porcine circovirus type 2 (PCV2) and porcine parvo virus (PPV) in 54 porcine aborted foetuses, stillborns and mummified foetuses.  Foetal  tissues of   heart, lung, spleen and lymphnodes were used for extraction of total DNA and the presence of viral  pathogens were confirmed through polymerase chain reaction (PCR) technique. Out of 54 samples, nine (16.6%) samples were positive for PCV2, eight (14.8%) samples were positive for PPV while co-infection with PCV2 and PPV was detected in four (7.4%) samples. The histopathological changes in the foetuses were found to be mostly necrosis in the cells of the developing organs viz. lungs, kidney, liver, heart, brain, spleen and lymphnodes. Lymphoid depletion was observed in the lymphoid follicles of the lymphnodes. Meningoencephalitis in the cerebrum was also seen in both PPV and PCV2 infected foetuses. The results indicated that PPV and PCV2 could be an important infectious agent in cases of porcine stillbirths, abortions and mummified foetuses.

REFERENCES


  1. Altherr, B., Zimmermann, P., Etschmann, B., Ritzmann, M., Heinritzi, K., Selbitz, H. J., Truyen, U. (2003). Detection of PCV 2 and PPV in aborted fetuses. 4th International symposium on emerging and re-emerging pig diseases—    Rome. 218–219

  2. Arnauld, C., Legeay, O., Laurian, Y., Thiery, R., Denis, M., Blandchard, P. and Jestin, A. (1998). Development of a PCR-    based method coupled with a microplate colorimetric assay for the detection of PPV and application to diagnosis in piglet tissues and human plasma. Molecular and Cellular Probes. 12: 407–416

  3. Ellis, J., Krakowka, S., Lairmore, M.; Haines, D., Bratanich, A., Clark, E., Allan, G., Konoby, C., Hassard, L., Meehan, B., Martin, K., Harding, J., Kennedy, S., McNeilly, F. (1999). Reproduction of lesions of postweaning multisystemic wasting syndrome in gnotobiotic piglets. Journal of Veterinary Diagnostic Investigation. 11: 3-14

  4. Hansen, M.S., Hjulsager, C.K., Bille-Hansen, V., Haugegaard, S., Dupont, K., Hogedal, P., Kunstmann, L., Larsen, L.E. (2010). Selection of method is crucial for the diagnosis of porcine circovirus type 2 associated reproductive failures. Veterinary Microbiology. 144: 203-209

  5. Hogg, A. and Levis, D.G. (1989). G89-926 swine reproductive problems: infectious causes. Historical Materials from University of Nebraska-Lincoln Extension. Paper 1381

  6. Holler, L.D. (1994). Diagnosis of swine abortions. Swine Health and Production. 2: 29-31

  7. Kim, J., Han, D., Choi, C., Chae, C. (2003). Simultaneous detection and differentiation between porcine circovirus and porcine parvovirus in boar semen by multiplex seminested polymerase chain reaction. Journal of Veterinary Medical Science. 65: 741-744

  8. Kim, J., Jung, K.; Chae, C. (2004). Prevalence of porcine circovirus type 2 in aborted fetuses and stillborn piglets. Veterinary Record. 155: 489-492.

  9. Kumar, S.K., Selvaraj, R., Hariharan, T., Chanrahassan, C. and Reddy, Y.K.M. (2014). Porcine Circovirus-2 an emerging disease of crossbred pigs in Tamil Nadu, India. International Journal of Science, Environment and Technology. 3:1268-1272

  10. Maldonado, J., Segalés, J., Martinez-Puig, D., Calsamiglia, M., Riera, P., Domingo, M., Artigas, C. (2005). Identification of viral pathogens in aborted fetuses and stillborn piglets from cases of swine reproductive failure in Spain. Veterinary Journal. 169: 454-456

  11. Mateusen, B., Maes, D.G., Van Soom, A., Lefebvre, D., Nauwynck, H.J. (2007). Effect of a porcine circovirus type 2 infection on embryos during early pregnancy. Theriogenology. 68: 896–901

  12. Mengeling, W. L. (2006). Porcine parvovirus. In: Straw, B., D’Allaire, S., Taylor, D., Zimmerman, J. (Eds.). Diseases of swine. Iowa State University Press, Ames, Iowa. 373-385

  13. Park, J.S., Kim, Y., Jung, K., Choi, C., Lim, J-K., Kim, S-H, Chae, C. (2005). Birth abnormalities in pregnant sows infected intranasally with porcine circovirus 2. Journal of Comparative Pathology.132: 130-144

  14. Pescador, C., Bandarra, P., Castro, L., Antoniassi, N.A.B., Ravazzolo, A.P., Sonne, L., Cruz, C.E.F., Driemeier, D. (2007). Co-infection by porcine circovirus type 2 and porcine parvovirus in aborted fetuses and stillborn piglets in southern Brazil. Pesquisa Veterinária Brasileira. 27: 425-429

  15. Pittman, S.J. (2008). Reproductive failure associated with porcine circovirus type 2 in gilts. Journal of Swine Health and Production. 16:144-148

  16. Rocha, D.L., Alberton G.C., Santos E.J.L.D. (2010). Identification of porcine circovirus type 2 and porcine parvovirus in porcine stillbirths and mummified fetuses from farms in Brazil. Ci. Anim. Bras., Goiânia. 11:600-606

  17. Rose, N., Blanchard, P., Cariolet, R., Grasland, B., Amenna, N., Oger, A., Durand, B., Balasch, M., Jestin, A., Madec, F. (2007). Vaccination of porcine circovirus type 2 (PCV2) infected sows against porcine parvovirus (PPV) and erysipelas: effect on post-weaning multisystemic wasting syndrome (PMWS) and on PCV2 genome load in the offspring. Journal of Comparative Pathology. 136: 133-144

  18. Sanchez, R.E., Nauwynck, H., McNeilly, F., Allan, G., Pensaert, M.B. (2001). Porcine circovirus 2 infection in swine fetuses inoculated at different stages of gestation. Veterinary Microbiology. 83:169-176

  19. Segalés, J., Allan, G.M., Domingo, M. (2006). Porcine circovirus diseases. In: Straw, B., D’Allaire, S., Taylor, D., Zimmerman, J. (Eds.). Diseases of swine. Ames, Iowa: Iowa State University Press. 299-307

  20. Sharma, R and Saikumar G. (2008). PCV 2 associated reproductive failure in Indian pigs. Indian Journal of Animal Science. 78:1239–1241

  21. Sharma, R. and Saikumar, G. (2010). Porcine parvovirus and porcine circovirus 2 associated reproductive failure and neonatal mortality in crossbred Indian pigs. Tropical Animal Health and Production. 42: 515–522

  22. Thomson, G.R. and Proszesky, L. (1994). PPV infection. In: Coetzer, J.A.W., Thomson, G.R., Tustin, R.C. (Eds). Infectious diseases of livestock, Oxford Univ. Press, South Africa. 2: 884–894

     
Reviewed By

Download Article
In this Article
    Contact us
    Follow us

    Editorial Board

    View all (0)