Indian Journal of Animal Research

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Research Article

Indian Journal of Animal Research, volume 51 issue 3 (june 2017) : 464-468

Histomorphology and scanning electron microscopy of the pharyngeal tonsil in goats

V. R. Indu*, K.M. Lucy, N. Ashok, S. Maya, V.L. Gleeja
1<p>Department of Veterinary Anatomy,&nbsp;College of Veterinary and Animal Sciences, Mannuthy- 680 651, India</p>
Cite article:- Indu* R. V., Lucy K.M., Ashok N., Maya S., Gleeja V.L. (2016). Histomorphology and scanning electron microscopy of the pharyngealtonsil in goats . Indian Journal of Animal Research. 51(3): 464-468. doi: 10.18805/ijar.8428.

ABSTRACT

Gross and histological studies were conducted on the pharyngeal tonsil of six male crossbred goats of six months of age. In the nasopharynx, pharyngeal tonsil was located on the caudal part of the pharyngeal septum and was 5.54±1.41cm long and 2.19±0.92cm wide. It presented numerous longitudinally arranged primary and secondary folds. Histologically the tonsil was lined by pseudostratified ciliated columnar epithelium comprising of 8-14 rows of nuclei of three types of cells, viz. basal, supporting and goblet cells. This epithelium was transformed at places into follicle-associated epithelium (FAE) and was characterized by decreased height of the epithelial cells, absence of cilia and goblet cells and heavy infiltration of lymphocytes through the interrupted basement membrane. The height of surface epithelium was 87.33± 1.20µm and that of follicle-associated epithelium was 52.33± 5.21µm. Propria-submucosa comprised of a central axis of loosely arranged connective tissue with dense aggregates of lymphoid tissue, fine blood capillaries and few nerve fibres folded around it. The cryptolymphatic units and tonsillar nodules of varying shape and dimensions constituted the majority of the lymphoid tissue. The average diameter of lymphoid nodules was 921.67±8.72µm and the lymphocyte count per nodule was 32233.23±324.24.  The average number of lymphatic nodules counted per field under low power magnification of microscope was 2.5±0.43 and the internodular distance was 29.83±1.40µm.  In scanning electron microscopy surface of the pharyngeal tonsil was covered by two types of epithelium viz., the ciliated respiratory surface epithelium and the FAE consisting predominantly of three types of non-ciliated microvillus cells. 

REFERENCES


  1. Anderson, J.C. (1974). The response of the tonsil and associated lymph nodes of gnotobiotic piglets to the presence of bacterial antigen in the oral cavity. J. Anat. 117: 191-198.

  2. Bancroft, J.D. and Gamble, M. (2003). Theory and Practice of Histological Techniques. (5th Ed.). Churchill Livingstone, New York, 796p. 

  3. Baykan, M., Celik, I., Gezici, H., Donmez, H., Eken, H., Sur, E. and Ozkan, Y.(2001). A light microscopic study on the uptake and transportation route of carbon particles in the canine palatine tonsil. Revue Méd. Vét. 152: 709-715.

  4. Billen, F., Peeters, D., Dehard, S., Day, M. J. and Clercx, C. (2006). Distribution of leucocyte subsets in the canine pharyngeal tonsil. J. Comp. Path. 135: 63-73.

  5. Casteleyn, C., Cornelissen, M., Simoens, P. and Broeck, V.W.(2010). Ultramicroscopic examination of the ovine tonsillar epithelia. Anat. Rec. 293: 879–888.

  6. Cocquyt, G., Baten, T., Simoens, P. and Broeck, W.V.D. (2005). Anatomical localisation and histology of the ovine tonsils. Vet. Immunol. Immunopathol. 107: 79–86.

  7. Casteleyn, C., Breugelmans, S., Simoens, P. and Broeck, V.W. (2011). The tonsils revisited: Review of the anatomical localization and histological characteristics of the tonsils of domestic and laboratory animals. Clin. Dev. Immunol. 21: 1-14.

  8. Hafeez, A., Khan, M.Y. and Minhas, L.A. (2009). A comparative histological study of the surface epithelium and high endothelial venules in the subepithelial compartments of human nasopharyngeal and palatine tonsils. J. Coll. Phys. Surg. Pakistan. 19: 333-337.

  9. Kumar, P. and Nagpal, S.K. (2007). Histology and histochemistry of the nasopharyngeal tonsil of sheep. Haryana Vet. 46: 75-79.

  10. Kumar, P. and Timoney, J.F.(2001). Light and electron microscopic studies on the nasopharynx and nasopharyngeal tonsil of the horse. Anat. Histol. Embryol. 30: 77–84.

  11. Kumar, P. and Kumar, P. (2004). Histology, histochemistry and scanning electron microscopy of the nasopharynx of the goat (Capra hircus). Haryana. Vet. 43: 51-55.

  12. Kumar, P., Kumar, P. and Kumar, S. (2006). Light and scanning electron microscopic studies on the nasopharyngeal tonsil of the goat. Indian J. Anim. Sci. 76: 452–455.

  13. Kumar, P., Kumar, P., Kumar, P. and Jain, R.K. (2011). Light and scanning electron microscopy of nasopharynx of sheep. Haryana. Vet. 49: 31-34.

  14. Kumar, P., Timoney, J.F. and Sheoran, A.S. (2001). M cells and associated lymphoid tissue of the equine nasopharyngeal tonsil. Equine Vet. J. 33: 224-230.

  15. Liu, Z., Yu, Q., Li, P. and Yang, Q. (2012). Histological and ultrastructural examinations of porcine tonsils. Anat. Rec. 295: 686–690.

  16. Luna, L.G. (1968). Manual of Histological Staining Methods of the Armed Forces Institute of Pathology. (3rd Ed.). Mc Graw-Hill Book Company, New York, 258p.

  17. Mair, T.S., Batten, E.H., Stokes, C.R. and Bourne, F.J. (1987). The histological features of the immune system of the equine respiratory tract. J. Comp. Pathol. 97: 575–586.

  18. Morgan, K.T., Jiang, X.Z., Patterson, D.L. and Gross, E.A. (1984). The nasal mucociliary apparatus. Correlation of structure and function in the rat. Am. Rev. Resp. Dis. 130: 275-281.

  19. Palmer, M.V., Stasko, J., Ray, W.T. and Thacker, T.C. (2011). Examination of the reticular epithelium of the bovine pharyngeal tonsil. Anat. Rec. 294: 1939–1950.

  20. Perry, M. and Whyte, A. (1998). Immunology of the tonsils. Immunol. Today. 19: 414–421.

  21. Singh, U.B. and Sulochana, S. 1996. Handbook of Histological and Histochemical Techniques. Premier Publishing House, Hyderabad, 111p.

  22. Tenorio, E.M.L. and Pabst, R. (2006). MALT structure and function in farm animals. Vet. Res. 37: 257–280.

  23. Toppets, V., Defaweux, V., Piret, J., Kirschvink, N., Grobet, L. and Antoine, N. (2011). Features of follicular dendritic cells in ovine pharyngeal tonsil: An in vivo and in vitro study in the context of Scrapie pathogenesis. Vet. Immunol. Immunopathol. 141: 26–32.

     
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