Characterization and validation of point mutation in exon 19 of CACNA2D1 gene in Karan Fries (Bos taurus x Bos indicus) cattle

DOI: 10.18805/ijar.5668    | Article Id: B-3078 | Page : 227-230
Citation :- Characterization and validation of point mutation in exon 19 of CACNA2D1 gene in Karan Fries (Bos taurus x Bos indicus) cattle .Indian Journal Of Animal Research.2017.(51):227-230

Ankit Magotra, I.D. Gupta*, Archana Verma, M.V. Chaudhari, Ashwani Arya, M.R. Vineeth, Rakesh Kumar and A. Sakthivel Selvan

Address :

Dairy Cattle Breeding Division, ICAR- National Dairy Research Institute, Karnal-132 001, India

Submitted Date : 23-07-2015
Accepted Date : 14-09-2015

Abstract

The objective of this study was to characterize and validate the candidate point mutation in Calcium channel, voltage-dependent, alpha-2/ delta subunit 1 (CACNA2D1) gene in Karan Fries (Bos taurus x Bos indicus) cattle. The CACNA2D1 gene reported as one of the potential candidate gene influencing Somatic cell Score and Mastitis. A PCR product of 249 bp amplifying the exon 19 and partial 18 and 19 intronic region of CACNA2D1 gene was digested with Hae III restriction enzyme to screen the reported point mutation. A monomorphic banding pattern with genotype AA was found in Karan Fries cattle. Sequencing was also carried out to characterize and explore insilico screened mutation in the nucleotide sequence of a particular region. The result indicates highly conserved sequence in Karan Fries cattle. The Phylogenetic tree revealed that Karan Fries cattle were closer to Bos taurus cattle, Bos mutus (Yak), and Bison bison (American buffalo) compared to other species.

Keywords

CACNA2D1 Karan Fries Monomorphic Mutation.

References

  1. Buitkamp, J., D. Ewald .,J. Masabanda .,M.D. Bishop. and R. Fries. (2003). FISH and RH mapping of the bovine alpha (2)/    delta calcium channel subunit gene (CACNA2D1). Animal Genetics, 34:302–318
  2. Daetwyler, H.D., F.S. Schenkel ., M. Sargolzaei . and J.B.M. Robinson. (2008). Genome scans in dairy cattle using a dense SNP map. J Dairy Sci., 91:3225–3236
  3. Edgar, C.Robert. and Muscle. (2004). Multiple sequence alignment with high accuracy and high throughput, Nucleic Acids Research, 32:1792- 1797.
  4. Gabashvili, I.S., B.H. Sokolowski ., C.C. Morton. and A.B. Giersch, (2007). Ion Channel Gene Expression in the Inner Ea. J. Assoc. Res. Otolaryngol., 8: 305–28.
  5. Hille. And Bertil. (2001). Ion Channels of Excitable Membranes (3rd Ed.). Sunderland, Mass: Sinauer Associates. ISBN 0-87893-321-2.
  6. Hou, GY., Z.R. Yuan., X. Gao., Y.L. Jun., H.J. Gao., J.B.Chen. and S.Z. Xu . (2010). Genetic polymorphisms of CACNA2D1 gene and their association with carcass and meat quality traits in cattle. Biochem Genet., 48:751–759
  7. Longeri. M., M. Polli., M.G. Strillacci., A.B. Samore. and M. Zanotti (2006). Short communication: quantitative trait loci affecting the somatic cell score onchromosomes 4 and 26 in Italian Holstein cattle. J Dairy Sci., 89:3175–3177
  8. Rosyara, P.N. 2006. Requirement of robust molecular marker technology for plant breeding applications. Journal of Plant Breed Gr., 1: 67
  9. Purves .(2001). Channels and Transporters. In Dale Purves, George J. Augustine, David Fitzpatrick, Lawrence. C. Katz, Anthony-Samuel LaMantia, James O. McNamara, S. Mark Williams, editors. Neuroscience., Chapter 4: (2nd ed.).
  10. Rupp, R. and D. Boichard. (2003). Genetics of resistance to mastitis in dairy cattle. Vet Res., 34:671–688
  11. Sambrook, J. and D. Russell. (2001). Cold Spring Harbor, NY: Cold Spring Harbor Laboratory. Molecular Cloning: a Laboratory Manual, 3rd edn.
  12. Tamura, K., D. Peterson., N. Peterson., G. Stecher., M. Nei. and S. Kumar . (2011). Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol., 28:2731-9
  13. Vicini, S.(1999). New perspectives in the functional role of GABA(A) channel heterogeneity. Mol. Neurobiol., 19: 97–110.
  14. Yuan, Z., J. Li., L. Zhang., C. Chen., X. Chen., X. Gao., J. Chen., H. Gao. and S. Xu. (2011b). Single nucleotide polymorphism of CACNA2D1 gene and its association with milk somatic cell score in cattle. Mol. Biol. Rep., 38: 5179-5183.
  15. Yuan, Z., J. Li., L. Zhang., C. Chen., X. Chen., X. Gao., J. Chen., H. Gao. and S. Xu. (2011a). Novel SNPs polymorphism of bovine CACNA2D1 gene and their association with somatic cell score. Afr. J. Biotechnol. 10: 1789-1793
  16. Zhang, Q., D. Boichard., I. Hoeschele., C. Ernst., A. Eggen., B. Murkve., M. Pfister-Genskow., L. A. Witte., F.E. Grignola., P. Uimari., G. Thaller., M.D. Bishop. (1998). Mapping quantitative trait loci for milk production and health of dairy cattlein a large outbred pedigree. Genetics 149:1959–1973

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