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Anthelmintic evaluation of Cucurbita pepo against gastrointestinal nematodes of sheep
First Online 03-03-2017|
The study was aimed at to investigate the anthelmintic efficacy of aqueous and ethanolic extracts of Cucurbita pepo against gastrointestinal nematodes of sheep by scientifically validated in vitro and in vivo tests. In vitro anthelmintic activity was determined by egg hatch assay, larval migration inhibition assay and larval development assay against eggs and larvae of mixed gastrointestinal nematodes of sheep. The extracts which were found to be effective in in vitro assays were further evaluated by in vivo faecal egg count reduction test against mixed species of sheep gastrointestinal nematodes with 3 different doses (125, 250 and 500 mg kg-1). Haematological and serum parameters were estimated before and after treatment to determine the impact of plant extracts on health of the animal. Aqueous extract of C. pepo demonstrated significant inhibition of egg hatch at 40 and 80 mg/ml. The ED50 value of egg hatch inhibition was 3.51mg/ml. In larval migration inhibition assay, both aqueous and ethanolic extracts of C. pepo demonstrated inhibition of larval migration and the LM50 was 1.75 and 0.32 mg/ml respectively. However, in larval development assay, both aqueous and ethanolic extracts showed only marginal inhibition of larval development at all concentrations tested. In faecal egg count reduction test (FECRT), aqueous extracts of C. pepo at 500 mg/kg caused significant reduction in eggs per gram (96.61 per cent) the reduction being higher than the result obtained with albendazole, the positive control (93.25 per cent). There was a significant difference in EPG reduction between sheep treated with albendazole and C. pepo. Haematological and serum parameters were not affected in both the treated and untreated sheep indicating that the extracts did not cause toxic effects. Based on the results obtained in this study, the aqueous extract of C. pepo possesses anthelmintic activity and could offer an alternative source for the control of gastrointestinal nematodes of sheep.
- Adama, K., Amadou, T., Man., N., Isidore., G. B., Valentin., B., Hamidou. T. H. and Gaston. B. M. A., (2012). In vitro anthelmintic activity of Leuceana leucocephala (Lam) De Wit. (Mimosaceae) and Gliricidia sepium (Jacq.) Kunth ex Steud (Fabaceae) leave extract on Haemonchus contortus ova and larvae. J. Chem. Pharm. Res., 4: 303-309.
- Ademola, I.O., Fagbemi. B. O. and Idowu. S. O. (2007). Anthelmintic efficacy of Nauclea latifolia extract against gastrointestinal nematodes of sheep. In vitro and in vivo studies. Afr. J. Trad. Compli. Alt. Med., 4: 148-156.
- Ademola, I.O. and Eloff, J.N. (2010). In vitro anthelmintic activity of Combretum molle (R. Br. ex G. Don) (Combretaceae) against Haemonchus contortus ova and larvae. Vet Parasitol., 169:198-203.
- Ahmed, M., Laing. M. D. and Nsahlai. I. V. (2014). In vivo effect of selected medicinal plants against gastrointestinal nematodes of sheep. Trop.Anim.Health.Pro., 46: 411-417.
- Amin, M. R., Mostofa., M., Islam. M. N. and Asgar. M. A. (2010). Effects of neem, betel leaf, devil’s tree, jute and turmeric against gastrointestinal nematodes in sheep. J. Bangladesh Agril. Univ., 8: 259-263.
- Baldo, R.C. (2001). Comparative efficacy of pine apple leaves bolus and albendazole against GIN of sheep. DVM Dissertation, College of veterinary medicine, university of the Philipppines at Los Barios, 38 pp.
- Behnke, J.M., Buttle., D. J., Stepek., G., Lowe. A. and Duce. I. R. (2008. Developing novel anthelmintics from plant cysteine proteinases. Parasites & Vectors., 1: 29.
- Bendixsen, T., H. T. Hanh., Vu. Van. Dong., Dinh. Van. Binh. and Phuong Song Lien. (2005). Proceedings of International Workshop on Small Ruminant Production and development in South East Asia held at Vietnam. 2 - 4, March. : pp. 97-99.
- Chiejina, S.N. (2001). The epidemiology of helminthic infections of domestic animals in the tropics with emphasis on fascioliasis and parasitic gastroenteritis. In: perspectives on helminthology (Chowdhury N, Tada I, Eds) Science Publishers, Inc Enfield.
- Coles, G.C., Bauer., C. Borgsteede., F. H. M. Geerts., S., Taylor. M. A. and Waller. P. J. (1992). World Association for the Advancement of Veterinary Parasitology methods for the detection of anthelmintic resistance in nematodes of Veterinary importance. Vet. Parasitol., 44: 35-44.
- Cowan, M. M. (1999). Plant products as antibacterial agents. Clinical Microbiol.Rev., 12: 564-582.
- Eguale, T., Tadesse. D. and Giday. M. (2011). In vitro anthelmintic activity of crude extracts of five medicinal plants against egg-hatching and larval development of Haemonchus contortus. J. Ethnopharmacol., 137: 108-113.
- Ferreira, L.E., Castro, P. M. N., Chagas, A. C. S., França, S. C. and Beleboni, R. O. (2013). In vitro anthelmintic activity of aqueous leaf extract of Annona muricata L. (Annonaceae) against Haemonchus contortus from sheep. Exp. Parasitol., 134: 327-332.
- Githiori, J.B., Hogland., J. Waller. P. J. and Baker. R.L. (2004). Evaluation of anthelmintic properties of some plants used as livestock dewormers against Haemonchus contortus infection in sheep. Parasitol., 129: 245-53.
- Hordegen, P., Hertzbeng., H., Helimann, J., Langhans, W., and Maurer. V., (2003). The anthelmintic efficacy of five plant products against gastrointestinal Trichostrongylids in artificially infected lambs. Vet.Parasitol., 117: 51-60.
- Hossain, S.A., Mostofa, M., Alam., M. N., Awal. M. A., and Ahmed. N. U., (1996). Comparative efficacy of modern anthelmintics and neem (leaves and seeds) in the treatment of bovine nematodiasis. Prog. Agric., 7: 29-33.
- Hubert, J. and Kerboeuf. D. A. (1992). Microlarval development assay for the detection of anthelmintic resistance in sheep nematodes. Vet. Rec., 130: 442-446.
- Jackson, F., Jackson. E. and Coop. R. L. (2001). Larval migration inhibition assay for determination of susceptibility of nematodes to levamisole. Practical exercise in Parasitology by David W.Halton, Jenzey M.Behnke and Ian Marshall, Cambridge University Press. : pp. 321 - 327.
- Jain, N.C. (1986). Schalm’s Veterinary Haematology. 4th Edn. (ed N. C. Jain) Lea and Febiger. : Philadelphia pp. 1221.
- Jorge, F.S.F., Peaden, P. and Keiser. J. (2011). In vitro trematocidal effects of crude alcoholic extracts of Artemisia annua, A. absinthium, Asimina triloba, and Fumaria officinalis, Trematocidal plant alcoholic extracts. Parasitol. Res., 109: 1585-1592.
- Kamaraj, C., Rahuman., A. A. Elango. G., Bagavan. A. and Zahir. A. A., (2011). Anthelmintic activity of botanical extracts against sheep gastrointestinal nematodes. Haemonchus contortus. Parasitol.Res., 109: 37-45.
- Laurence, D. R. and Banarach. A.L. (1964). Evaluation of drug activities Pharmacometrics. Academic Press, London.
- Lorena, M., Bde. and Bevilaqua. C. M. L. (2011). Effects of Myracrodruon urundeuva extracts on egg hatching and larval exsheathment of Hamonchus contortus. Parasitol. Res., 109: 893-898.
- MAFF. (1971). Manual of Veterinary Parasitological laboratory Techniques. Ministry of Agriculture, Fisheries and Food, Technical Bulletin, HMSO, London, pp. 36-42.
- Mesquita, Mde. A. and Batista. Je. S. J. (2013). Anthelmintic activity of Eucalyptus staigeriana encapsulated oil on sheep gastrointestinal nematodes. Parasitol. Res., 112: 3161-3165.
- Nogueira, F. A. and Fonseca. L. D. (2012). Anthelminthic efficacy of banana crop residues on gastrointestinal nematodes of sheep: in vitro and in vivo tests. Parasitol. Res., 111: 317-323.
- Nogueira, F. A., Fonseca. L. D. and Bda. Silva. R. (2012) . In vitro and in vivo efficacy of aqueous extract of Caryocar brasiliense camb to control gastrointestinal nematodes in sheep. Parasitol. Res., 111: 325-330.
- Ogbonnia, O.S., Nkemehule. F. E. and Anyika. E. N. (2009). Evaluation of acute and sub chronic toxicity of Stachytarpheta angustilolia (Mill) Vahl (Fam.Verbanaceae) extract in animals. Afr. J. Biotech., 8: 1793-1799.
- Onyeyili, P.A., Nwosu., C. O. Amin. J. D. and Jibike. J. I. (2001). Anthelmintic activity of crude aqueous extract of Nauclea latifolia stems bark against ovine nematodes. Fitoterapia., 72: 12-21.
- Oze, G.O., Nwanjo. H. U. and Onyeze. G. O. (2007). Nephrotoxicity caused by the extract of Alstonia boonei (De Wild) stem bark in Guinea pigs. Internet J. Nutr. Wellness., 3: 2.
- Rao, C, V., A.R.Verma., P.K.Gupta. and M.Vijayakumar. (2007). Antiinflammatory and anti-nociceptive activities of Fumaria indica whole plant extracts om experimental animals. Acta. Pharma., 57: 491-498.
- Rob, S., M. Mostafa., Awal., M. A., Shahiduzzaman. M. and Sardar. S. A. (2004). Comparative efficacy of albendazole (Endokil) and neem (Azadirachta indica) leaves extract against haemonchosis in sheep. Prog.Agric., 15: 33-39.
- Ruben, D. K., Baltini., S., Andrew. W., and Abdulrahman. F. I., (2011). Preliminary phytochemical screening and in vitro anthelmintic effects of aqueous extracts of Salvadora persica and Terminalia avicennoides against strongyle nematodes of small ruminants in Nigeria. J. Ani. Vet. Sci., 10: 437-442.
- Schalm, O.W., Jain. N. C. and Carrol. E. J. (1975). Veterinary Haematology. 3rd Edn. Lea and Febiger. Philadelphia pp. 15-81.
- Snedecor, G.W. and Cochran. W. G. (1976). Statistical Methods. 1976: 6th Edn. Oxford and IBH Publishing Co., Calcutta.
- Soro, M., Kone. W. M. and Bonfoh. B. (2013). In vivo anthelmintic activity of Anogeissus leicarpus Guill & Perr (Combretaceae) against nematodes in naturally infected sheep. Parasitol. Res., 112: 2681-2688.
- Strickland, V.J., Krebs. G. L. and Potts. W. (2007). Pumpkin kernel and garlic as alterative treatments for the control of Haemonchus contortus in sheep. CSIRO publishing- Animal Production Science.
- Sujith, S., Sreedevi., R. Priya., M. N. Deepa. C. K., Darsana. U., Sreeshitha. S. G., Suja. R. S. and Juliet. S. (2015). Anthelmintic activity of three Indian medicinal plants. Int.J.Phcog. Phytochem.Res ., 7: 361-364.
- Villasener, I.M., Cabrera., M.A. Meneses., K.B. Rivera. V.R.R. and Vilasenor. R.M. (1998). Comparative antidiabetic activities of some medicinal plants. J.Ethnopharmacol., 22: 1-2.
- Wang, L and Waller. C. L. (2006). Recent advances in extraction of naturaceuticals from plants. Trends Food Sci. Technol., 17: 300-312.
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