Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

  • Print ISSN 0367-6722

  • Online ISSN 0976-0555

  • NAAS Rating 6.50

  • SJR 0.263

  • Impact Factor 0.4 (2024)

Frequency :
Monthly (January, February, March, April, May, June, July, August, September, October, November and December)
Indexing Services :
Science Citation Index Expanded, BIOSIS Preview, ISI Citation Index, Biological Abstracts, Scopus, AGRICOLA, Google Scholar, CrossRef, CAB Abstracting Journals, Chemical Abstracts, Indian Science Abstracts, EBSCO Indexing Services, Index Copernicus
Submit

Research Article

Indian Journal of Animal Research, volume 51 issue 3 (june 2017) : 510-517

Molecular and histopathological evaluation of the efficacy of Saponified and Freund’s incomplete adjuvanted paratuberculosis killed vaccine in murine model

Jubeda Begum, Pranabananda Das, Madhu Cholenahalli Lingaraju
1<p><br /> Division of Bacteriology and Mycology,&nbsp;Indian Veterinary Research Institute, Izatnagar-243 122 (U.P.), India.</p>
Cite article:- Begum Jubeda, Das Pranabananda, Lingaraju Cholenahalli Madhu (2017). Molecular and histopathological evaluation of the efficacy of Saponifiedand Freund&rsquo;s incomplete adjuvanted paratuberculosiskilled vaccine in murine model . Indian Journal of Animal Research. 51(3): 510-517. doi: 10.18805/ijar.v0iOF.7602.

ABSTRACT

Johne’s disease (JD) is caused by Mycobacterium avium subspecies paratuberculosis (MAP). Vaccination is regarded as the only cost effective method for controlling JD. Experimental trial for 11 months was carried out by dividing mice into four different groups. Group I- saponified MAP killed vaccine; Group II- Freund’s incomplete adjuvant (FIA) MAP killed vaccine; Group III-saponin adjuvant control; Group IV- FIA control. Mice of all the groups were challenged with 108 colony forming unit live MAP organisms on 40th day after vaccination. A total of 188 samples (52 faecal, 76 sera and 60 tissue samples) were collected from different groups of mice at different interval for monitoring MAP load by Ziehl Neelsen’s staining and nested PCR along with tissue histopathology. Both the vaccinated groups (Group I and II) showed encouraging results in comparison to adjuvant control groups. There was reduction in faecal shedding (p<0.01), tissue load of MAP and less granuloma formation in both the vaccinated groups, however mice vaccinated with saponified MAP antigen gave better result in comparison to FIA vaccinated mice. Results of this study suggested that, saponified MAP killed vaccine in particular might reduce the overall burden of JD and show promising result in future.

REFERENCES


  1. Bannantine, J.P., Everman, J.L., Rose, S.J., Babrak, L., Katani, R., Barletta, R.G., Talaat, A.M., Gröhn, Y.T., Chang, Y.F., Kapur, V., Bermudez, L.E. (2014). Evaluation of eight live attenuated vaccine candidates for protection against challenge with virulent Mycobacterium avium subspecies paratuberculosis in mice. Front. Cell. Infect. Microbiol. 1:4:88.

  2. Carvalho, I.A., Campos, V.E.B., Souza, I.M., Zanardo, L.G., Filho, J.D.R., Gomes, M.J.P., Moreira, M.A.S. (2012). Diagnosis of paratuberculosis in cattle: microbiological culture, serology and PCR. Braz. J. Microbiol. 43: 581–5.

  3. Castellanos, E., de, J.L., Dominguez, L., Aranaz, A. (2012). Progress in molecular typing of Mycobacterium avium subspecies paratuberculosis. Res. Vet. Sci. 92:169 -79.

  4. Chiodini, R.J., Chamberlin, W.M., Sarosiek, J., McCallum, R.W. (2012). Crohn’s disease and the mycobacteriosis a quarter century later: Causation or simple association. Crit. Rev. Microbiol.38: 52-93.

  5. Cho, J., Tauer, L.W., Schukken, Y.H., Gómez, M.I., Smith, R.L., Lu, Z., Grohn, Y.T. (2012). Economic analysis of Mycobacterium avium subspecies paratuberculosis vaccines in dairy herds J. Dairy Sci. 95: 1855-72.

  6. Debroy, B., Tripathi, B.N., Sonawane, G.G., Bind, R.B. (2012). Detection of Mycobacterium avium subspecies paratuberculosis (MAP) in alcohol-fixed tissues of sheep by ISMav2 gene PCR and its comparison with histopathology, bacterial culture and IS900 PCR. Small Ruminant Res. 105: 329-34.

  7. Dhand, N.K., Johnson, W.O., Eppleston, J., Whittington, R.J., Windsor, P.A. (2013). Comparison of pre- and post-vaccination ovine Johne’s disease prevalence using a Bayesian approach. Prev. Vet. Med. 111: 81-91.

  8. Fridriksdottir, V., Gunnarsson, E., Sigurdarson, S., Gudmundsdottir, K.B. (2000). Paratuberculosis in Iceland: Epidemiology and control measures, past and present. Vet Microbiol;77:263-7.

  9. Garg, R., Patil, P.K., Singh, S.V., Sharma, S., Gandham, R.K., Singh, A.V., Filia, G., Singh, P.K., Jayaraman, S., Gupta, S., Chaubey, K.K., Tiwari, R., Saminathan, M., Dhama, K., Sohal, J.S. (2015). Comparative evaluation of different test combinations for diagnosis of Mycobacterium avium subspecies paratuberculosis infecting dairy herds in India. Biomed. Res. Int. 983-978.

  10. Groenendaal, H., Zagmutt, F.J., Patton, E.A., Wells, S.J. (2015). Cost-benefit analysis of vaccination against Mycobacterium avium ssp. paratuberculosis in dairy cattle, given its cross-reactivity with tuberculosis tests. J. Dairy Sci. 98: 6070-84.

  11. Hinesa, M.E., 2nd, Stabel, J.R., Sweeney, R.W., Griffin, F., Talaat, A.M., Bakker, D. (2007). Experimental challenge models for Johne’s disease: A review and proposed international guidelines. Vet. Microbiol. 122:197-222.

  12. Hinesb, M.E., 2nd, Stiver, S., Giri, D., Whittington, L., Watson, C., Johnson, J. (2007). Efficacy of spheroplastic and cell-    wall competent vaccines for Mycobacterium avium subsp. paratuberculosis in experimentally-challenged baby goats. Vet. Microbiol. 120: 261-83.

  13. Jayaraman, S., Jain, M., Dhama, K., Singh, S.V., Datta, M., Jain, N., Chaubey, K.K., Gupta, S., Aseri, G.K., Khare, N., Yadav, P., Bhatia, A.K., Sohall, J.S. (2016). DIVA technology: indispensable tool for the control of Johne’s disease. JEBAS. 4:16-25.

  14. Knust, B., Patton, E., Ribeiro-Lima, J., Bohn, J.J., Wells, S.J. (2013). Evaluation of the effects of a killed whole-cell vaccine against Mycobacterium avium subsp paratuberculosis in 3 herds of dairy cattle with natural exposure to the organism. J. Am. Vet. Med. Assoc. 242: 663–9.

  15. Koets, A.P., Eda, S., Sreevatsan, S. (2015). The within host dynamics of Mycobacterium avium ssp. paratuberculosis infection in cattle: where time and place matter. Vet Res. 46: 61.

  16. Kurade, N.P., Tripathi, B.N., Rajukumar, K., Parihar, N.S. (2004). Sequential development of histologic lesions and their relationship with bacterial isolation, fecal shedding, and immune responses during progressive stages of experimental infection of lambs with Mycobacterium avium subsp. paratuberculosis. Vet. Pathol. 41: 378-87.

  17. Lindblad, E.B. (2000). Freund’s adjuvants. In: O’Hagan DT ed. Methods in Molecular Medicine: Vaccine Adjuvants—    Preparation Methods and Research Protocols. Totowa NJ: Humana Press. p 49–63.

  18. McNees, A.L., Markesich, D., Zayyani, N.R., Graham, D.Y. (2015). Mycobacterium paratuberculosis as a cause of Crohn’s disease. Expert. Rev. Gastroenterol. Hepatol. 9:12.1523-34.

  19. OIE Terrestrial Manual. (2014) Chapter 2.1.11. Paratuberculosis (Johne’s disease).

  20. Reddacliff, L., Eppleston, J., Windsor, P., Whittington, R., Jones, S. (2006). Efficacy of a killed vaccine for the control of paratuberculosis in Australian sheep flocks. Vet. Microbiol. 11:577-90.

  21. Saminathan, M., Rana, R., Ramakrishnan, M.A., Karthik, K., Malik, Y.S., Dhama, K. (2016). Prevalence, diagnosis, management and control of important diseases of ruminants with special reference to Indian scenario. JEBAS. 4: 338-67.

  22. Saxegaard F, Fodstad FH. (1985). Control of paratuberculosis (Johne’s disease) in goats by vaccination. Vet Rec. 116:    439-41.

  23. Senthil Kumar, T.M.A., Parthiban, M., Ganesan, P.I., Shrine Nagalakshmi, K., Ramadass, P. (2014). Use of nested PCR for the detection of Mycobacterium avium subsp. paratuberculosis in faecal samples. Indian Vet. J. 91: 27-9.

  24. Sigurdsson, B. (1960). A killed vaccine against paratuberculosis (Johnes disease) in sheep. Am. J. Vet. Res. 21: 54-67.

  25. Sikandar, A., Cheema, A.H., Younus, M., Aslam, A., Zaman, M.A., Rehman, T. (2012). Histopathological and serological studies on paratuberculosis in cattle and buffaloes. Pakistan. Vet. J. 32: 547-51.

  26. Singh, S.V., Singh, P.K., Singh, A.V., Sohal, J.S. Sharma, M.C. (2010). Therapeutic effects of a new Indigenous Vaccine developed using novel native “Indian Bison type” genotype of Mycobacterium avium subspecies paratuberculosis for the control of clinical Johne s disease in naturally infected goatherds in India. Vet. Med. Int. 351846

  27. Singh, S.V., Singh, A.V., Singh, P.K., Gupta, S., Singh, H., Singh, B., Vinodh Kumar, O.R., Rajendiran, A.S., Swain, N., Sohal, J.S. (2013). Evaluation of Indigenous vaccine developed using “Indian Bison Type” genotype of Mycobacterium avium subspecies paratuberculosis strain “S5” of goat origin in a sheep flock endemic for Johne’s disease: A three years trial in India. WJV. 3: 52-59

  28. Sohal, J.S., Singh, S.V., Singh, B., Thakur, S., Aseri, G.K., Jain, N., Jayaraman, S., Yadav, P., Khare, N., Gupta, S., Chaubey, K.K., Dhama, K. (2015). Control of Paratuberculosis: Opinions and Practices. Adv. Anim. Vet. Sci. 3:156-63.

  29. Stabel, J.R., Barnhill, A., Bannantine, J.P., Chang, Y.F., Osmanc, M.A. (2012). Evaluation of protection in a mouse model after vaccination with Mycobacterium avium subsp. paratuberculosis protein cocktails. Vaccine. 31: 127–34.

  30. Stills Jr., H.F., (2005). Adjuvants and antibody production: dispelling the myths associated with Freund’s complete and other adjuvants. ILAR J. 46: 280-93.

  31. Sweeney, R.W., Collins, M.T., Koets, A.P., McGuirk, S.M., Roussel, A.J. (2012). Paratuberculosis (Johne’s disease) in cattle and other susceptible species. J. Vet. Intern. Med. 26: 1239-50

  32. Tewari, D., Hovingh, E., Linscott, R., Martel, E., Lawrence, J., Wolfgang, D., Griswold, D. (2014). Mycobacterium avium subsp. paratuberculosis antibody response, fecal shedding, and antibody cross-reactivity to Mycobacterium bovis in M. avium subsp. paratuberculosis-infected cattle herds vaccinated against Johne’s disease. Clin. Vaccine Immunol. 21: 698-703. 
Reviewed By

Download Article
In this Article
    Contact us
    Follow us

    Editorial Board

    View all (0)