Indian Journal of Animal Research

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Research Article

Indian Journal of Animal Research, volume 51 issue 3 (june 2017) : 510-517

Molecular and histopathological evaluation of the efficacy of Saponified and Freund’s incomplete adjuvanted paratuberculosis killed vaccine in murine model

Jubeda Begum, *, Pranabananda Das, Madhu Cholenahalli Lingaraju
1<p><br /> Division of Bacteriology and Mycology,&nbsp;Indian Veterinary Research Institute, Izatnagar-243 122 (U.P.), India.</p>
Cite article:- Begum Jubeda, *, Das Pranabananda, Lingaraju Cholenahalli Madhu (2017). Molecular and histopathological evaluation of the efficacy of Saponifiedand Freund&rsquo;s incomplete adjuvanted paratuberculosiskilled vaccine in murine model . Indian Journal of Animal Research. 51(3): 510-517. doi: 10.18805/ijar.v0iOF.7602.

ABSTRACT

Johne’s disease (JD) is caused by Mycobacterium avium subspecies paratuberculosis (MAP). Vaccination is regarded as the only cost effective method for controlling JD. Experimental trial for 11 months was carried out by dividing mice into four different groups. Group I- saponified MAP killed vaccine; Group II- Freund’s incomplete adjuvant (FIA) MAP killed vaccine; Group III-saponin adjuvant control; Group IV- FIA control. Mice of all the groups were challenged with 108 colony forming unit live MAP organisms on 40th day after vaccination. A total of 188 samples (52 faecal, 76 sera and 60 tissue samples) were collected from different groups of mice at different interval for monitoring MAP load by Ziehl Neelsen’s staining and nested PCR along with tissue histopathology. Both the vaccinated groups (Group I and II) showed encouraging results in comparison to adjuvant control groups. There was reduction in faecal shedding (p<0.01), tissue load of MAP and less granuloma formation in both the vaccinated groups, however mice vaccinated with saponified MAP antigen gave better result in comparison to FIA vaccinated mice. Results of this study suggested that, saponified MAP killed vaccine in particular might reduce the overall burden of JD and show promising result in future.

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