Indian Journal of Animal Research

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Research Article

Indian Journal of Animal Research, volume 51 issue 2 (april 2017) : 275-279

Characterization of Exon 3 of PROP1 gene and Screening of H173R polymorphism in Karan Fries bulls

Vineeth M.R, I. D. Gupta*, Archana Verma, Ankit Magotra, Rakesh Kumar, Nishant Verma, Santosh Kumari
1<p>Dairy Cattle Breeding Division,&nbsp;ICAR-National Dairy Research Institute, Karnal-132 001, India.</p>
Cite article:- M.R Vineeth, Gupta* D. I., Verma Archana, Magotra Ankit, Kumar Rakesh, Verma Nishant, Kumari Santosh (2016). Characterization of Exon 3 of PROP1 gene and Screening ofH173R polymorphism in Karan Fries bulls . Indian Journal of Animal Research. 51(2): 275-279. doi: 10.18805/ijar.9498.

ABSTRACT

Present study was done in thirty Karan Fries bulls to characterize the Exon 3 of PROP1 gene and to screen for the H173R polymorphism as well as other variations including reported and novel SNPs in the targeted region. The exon 3 was characterized by sequencing the amplicons obtained after PCR amplification using custom designed primers. The BLAST analysis of the obtained sequence yielded 100% and 99% homology with sequences of Bos taurus and bison respectively. The multiple alignment of the target region sequence with Bos taurus reference sequence revealed that the bulls under the study were free of H173R mutations. No variations were observed thus giving the targeted region a highly conserved one.

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