Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

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Indian Journal of Animal Research, volume 51 issue 2 (april 2017) : 347-349

Comparison of different diagnostic test to detect feline panleukopenia virus among cats in Kerala, India

Raheena Koulath P., P. M. Priya*, Binu K Mani, M. Mini, Usha Narayana Pillai
1<p>Department of Veterinary Microbiology,&nbsp;College of Veterinary and Animal Sciences, Mannuthy, Thrissur &ndash; 680 651, India.</p>
Cite article:- P. Koulath Raheena, Priya* M. P., Mani K Binu, Mini M., Pillai Narayana Usha (2016). Comparison of different diagnostic test to detect feline panleukopenia virus among cats in Kerala, India . Indian Journal of Animal Research. 51(2): 347-349. doi: 10.18805/ijar.10975.

Feline panleukopenia (FPL) is an acute viral infection of domestic and wild felids causing high mortality among non immune kittens. Commercially available immunochromatographic (IC) strips, haemagglutination (HA) test and polymerase chain reaction (PCR) were employed to detect FPLV. In the current study IC strip test and HA test were compared with PCR. A total of 27 faecal samples from cats clinically suspected for FPL infection were collected from five districts of Kerala, India. Out of 27 samples tested, 10 were positive by IC strips, while 8 and 21 samples were found positive by HA test and PCR, respectively. On statistical analysis, specificity of IC strip and HA test versus PCR was excellent (100 per cent), whereas sensitivity was poor. In comparison with PCR, sensitivity for IC strip test and HA test was 47.6 per cent and 38.1 per cent, respectively. The study showed PCR assay as a sensitive, specific and rapid technique for FPLV detection in cats using faecal specimens.

  1. Clemens, D. L and J. O. Carlson. (1989). Regulated Expression of the Feline Panleukopenia Virus P38 Promoter on Extrachromosomal FPV/EBVChimeric Plasmids. J. Virol. 2737-2745.

  2. Deepa, P.M. (1999). Seroprevalence and diagnosis of canine parvoviral infection. M.V.Sc thesis, Kerala Agricultural University, Vellanikkara, 159 p.

  3. Desario, C., Decaro, N., Campolo, M., Cavalli, A., Cirone, F., Gabriella, E., Martella, V., Lorusso, E., Camero, M. and Buonavoglia, C. (2005). Canine parvovirus infection: which diagnostic test for the virus? J. Virol. Methods. 126: 179-185.

  4. Greene, C. E. (1998). Feline panleukopenia. In: Greene, CE (ed.), Infectious Diseases of the Dog and Cat. (2nd Ed.). W. B. Saunders Co. Philadelphia, pp: 52-57.

  5. Hong, C., Decaro, N., Desario, C., Tanner, P., Pardo, M.C., Sanchez, S., Buonavoglia, C. and Saliki, J.T. (2007). Occurrence of canine parvovirus type 2c in the United States. J. Vet. Diagn. Invest. 19: 535-539.

  6. Martyn, J.C., Davidson, B.E. and Studdert, M.J. (1990). Nucleotide sequence of feline panleukopenia virus: comparison with canine parvovirus identifies host-specific. J. Gen. Virol. 71: 2747-2753.

  7. Mathew, J. (2004). Polymearse chain reaction for the detection of canine parvovirus in faeces of dogs. M.V.Sc thesis, Kerala Agricultural University, Thrissur, Kerala, 114 p.

  8. Meenu, B. (2011). Detection of antigenic variants of canine parvovirus by polymerase chain reaction and sequencing. M.V.Sc. thesis, Kerala Veterinary and Animal Sciences University, Pookode, 166p.

  9. Mochizuki, M., Harasawa, R. and Nakatani, H. (1993). Antigenic and genomic variabilities among recently prevalent parvoviruses of canine and feline origin in Japan. Vet. Microbiol. 38: 1–10.

  10. Mochizuki, M., Horiuchi, M., Hiragi, H., Gabriel, M.C., Yasuda, N. and Uno, T. (1996). Isolation of canine parvovirus from a cat manifesting clinical signs of feline panleukopenia. J. Clin. Microbiol. 34: 2101-2105.

  11. Mosallanejad, B., Avizeh, R. and Ghorbanpoor, N. M. (2009). Antigenic detection of Feline Panleukopenia virus in diarrhoeic companion cats in Ahvaz area. Iranian. J. Vet. Res. 10: 289-293.

  12. Parrish, C.R., Aquadro, C.F. and Carmichael, L.E. (1988). Canine host range and a specific epitope map along with variant sequences in the capsid protein gene of canine parvovirus and related feline, mink and raccoon parvoviruses. J. Virol. 166: 293-307.

  13. Parthiban, M., Aarthi, K.S., Balagangatharathilagar, M. and Kumanan, K. (2014). Evidence of feline panleukopenia infection in cats in India. Virus. Dis. 10: 231-236.

  14. Uwatoko, K., Sunairi, M., Nakajima, M., and Kohichi, Y. (1995). Rapid method utilizing the polymerase chain reaction for detection of canine parvovirus in feces of diarrheic dogs. Vet. Microbiol. 43: 315-323.

  15. Vakili, N., Mosallanejad, B., Avizeh, R., Shapouri, S.M.R. and Pourmahdi, M. (2014). A comparison between PCR and Immunochromatography assay (ICA) in diagnosis of hemorrhagic gastroenteritis caused by Canine parvovirus. Arch. Razi. Inst. 69: 27-33.


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