Indian Journal of Animal Research

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Research Article

Indian Journal of Animal Research, volume 50 issue 1 (february 2016) : 101-104

Immunohistochemical localization of vasoactive intestinal peptide in bursa of fabricius of Chinese yellow quail

Min He*, Xiaoxia Liang, Kaiyu Wang, Haibo Pu, Yaodong Hu, Gang Ye, Xue Li, Ling Liu
1<p>Animal&rsquo;s Medical College, Sichuan Agricultural University,&nbsp;Ya&rsquo;an-625014, China.</p>
Cite article:- He* Min, Liang Xiaoxia, Wang Kaiyu, Pu Haibo, Hu Yaodong, Ye Gang, Li Xue, Liu Ling (2015). Immunohistochemical localization of vasoactive intestinal peptide in bursa of fabricius of Chinese yellow quail . Indian Journal of Animal Research. 50(1): 101-104. doi: 10.18805/ijar.7093.

ABSTRACT

This study aimed to reveal the distribution and morphological characteristics of vasoactive intestinal peptide (VIP) at different ages in the bursa of  Fabricius (BF) in Chinese Yellow Quail. The distribution and morphological characteristics of VIP of  BF in Chinese yellow quails at different ages (0, 1, 3, 5, 9, 13, 17, 21, 25, 29, 32 and 36 weeks) were observed using the immunohistochemical streptavidin-biotin complex method after obtaining the sections and subsequent immunohistochemical staining. Then semi-quantitative analysis of the mean optical density (MOD), integral optical density (IOD) and mean gray value (MGV) for VIP immunoreactivity was performed through Image-Pro Plus 6.0 software. VIP was observed at all ages in Chinese yellow quail’s BF. It formed a long fusiform, oval or granular type structures with clear outline mainly localized in the submucosa, muscular layer and serosal connective tissue. At 25 weeks of age, the MOD value of VIP-positive cells reached the maximum, and then gradually reduced, while the MGV value was the lowest. We observed the localization and morphological characteristics of VIP in BF of Chinese yellow quail. VIP was mainly localized in the submucosa, muscular layer and serosal connective tissue of BF. Findings of this study may provide further understanding of the function of VIP in BF of Chinese yellow quails.

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