Chief EditorK.M.L. Pathak
Print ISSN 0367-6722
Online ISSN 0976-0555
NAAS Rating 6.43
Impact Factor 0.5 (2023)
Single nucleotide polymorphisms in Heat Shock Protein (HSP) 90AA1 gene and their association with heat tolerance traits in Sahiwal cows
First Online 22-12-2015|
Heat Shock Proteins (HSPs) are group of proteins which are synthesized during heat stress. HSP genes have been reported to be associated with heat tolerance and production performance in cattle. HSP90AA1 gene has been mapped on Bos taurus autosome 21 (BTA 21) and spans nearly 5368 bp comprising 11 exons out of which first exon does not translate. The present study was carried out in Sahiwal cows (n=100) with the objectives to identify SNPs in targeted regions (exon 3, 7, 8 & 11) of HSP90AA1 gene and to analyze their association with heat tolerance traits in Sahiwal cows. Respiration rate (RR) and rectal temperature (RT) was recorded once during probable extreme hours in winter, spring and summer season. Further, heat tolerance coefficient (HTC) also calculated to see the adaptability of the animals during the period of heat stress. On the basis of comparative sequence analysis, total five SNPs were revealed at position of A1209G, A3292C, T4935C, T5218C and A5224C in the targeted region of HSP90AA1 gene. Out of these, only two SNPs at A1209G and A3292C loci were found significantly associated with heat tolerance traits in Sahiwal cows. Whereas, THI has a highly significant associated with RR, RT and HTC in all the seasons. At A1209G locus in Sahiwal cows for traits RR, genotype AA (18.40±0.46a), AG (19.60±0.85b) and GG (21.18± 0.64ab) and trait HTC AA (1.78±0.04a), AG (1.85±0.03b) and GG (1.91± 0.02c) differ significantly (p<0.01) while trait RT, AG (38.32±0.10b) and GG (38.27±0.08ab) didn’t differ significantly. For a locus A3292C for traits RR, genotype AA (21.55±1.01a), AC (19.66±1.59b) and CC (18.40±1.03c) differ significantly (p<0.05), while traits RT, genotype AA (38.41±0.12), AC (38.28±0.20) and CC (37.96±0.15) didn’t differ significantly. For trait HTC, AA (1.93±0.04a), AC (1.85±0.06b) and CC (1.79±0.05c) found significantly (p<0.05) differ with each other. Our study indicated that Sahiwal cows of AA and AC genotype had better thermo-tolerance capacity, which had been useful for genetic improvement of Sahiwal cattle for heat tolerance traits.
- Beatty, D.T., Barnes, A., Taylor, E., Pethick, D., McCarthy, M. and Maloney, S.K. (2006) Physiological responses of Bos taurus and Bos indicus cattle to prolonged, continuous heat and humidity. J. Anim. Sci., 84: 972–985
- Benezra, M. V. (1954). A New Index for Measures the Adaptability of Cattle to Tropical condition. Proc. J. Anim. Sci., 13: 1015.
- Berry, I.L., Shanklin, M. D. and Johnson, H. D. (1964). Dairy shelter design based on milk production decline as affected by temperature and humidity. Trans. Am. Soc. Ag. Eng., 7: 329-331.
- Charoensook, R., Gatphayak, K., Sharifi, A.R., Chaisongkram, C., Brenig, B. and Knorr, C. (2013). Polymorphisms in the bovine HSP90AB1 gene are associated with heat tolerance in Thai indigenous cattle. Trop. Anim. Health. Prod., 44: 921– 928
- Chen, B., Zhong, D. and Monteiro, A. (2006). Comparative genomics and evolution of the Hsp90 family of genes across all kingdoms of organisms. BMC. Genomics, 7: 156-167.
- Collier, R.J., Collier, J.L., Rhoads, R.P. and Baumgard, L.H. (2008). Invited Review: Genes involved in the bovine heat stress response. J. Dairy. Sci., 91:445–454.
- Das, R., Gupta, I.D., Verma, A., Singh, A., Chaudhari, M.V., Sailo, L., Upadhyay, R.C. and Goswami, J. (2015). Genetic polymorphisms in ATP1A1 gene and their association with heat tolerance in Jersey crossbred cows. Ind. J. Dai. Sci., 68: 50-54
- Deb, R., Sajjanar, B. and Singh U. (2013). Promoter variants at AP2 box region of Hsp70.1 affect thermal stress response and milk production traits in Frieswal cross bred cattle. Gene., 09:37.
- Gaughan, J.B., Mader, T.L., Holt, S.M., Sullivan, M.L. and Hahn, G.L. (2010). Assessing the heat tolerance of 17 beef cattle genotypes. Int. J. Biom., 54: 617–627.
- Hansen, P.J. (2004). Physiological and cellular adaptations of zebu cattle to thermal stress. Intl. J. Anim. Sci., 77: 36-50.
- Hoffmann I. (2010). Climate change and characterization, breeding and conservation of animal genetic resources. Anim. Gen., 41:32–46.
- Kregel, K.C. (2002). Heat shock proteins: modifying factors in physiological stress responses and acquired thermo tolerance. J. Appl. Physiol., 92: 2177–2186.
- Lacetera, N., Bernabucci, U., Scalia, D., Basiricò, L., Morera, P. and Nardone, A. (2006). Heat stress elicits different responses in peripheral blood mononuclear cells from Brown Swiss and Holstein cows. J. Dairy. Sci., 89: 4606–4612.
- Li, Q., Han, J., Du, F., Ju, Z., Huang, J., Wang, J., Li, R., Wang, C. and Zhong, J. (2011). Novel SNPs in HSP70A1A gene and the association of polymorphisms with thermo tolerance traits and tissue specific expression in Chinese Holstein cattle. Mol. Bio. Report., 38: 2657–2663.
- Liu, Y., Li, D., Li, H., Zhou, X. and Wang G. A. (2011). Novel SNP of the ATP1A1 gene is associated with heat tolerance traits in dairy cows. Mol. Bio. Rep., 38:83–88.
- Marai, I. F., M., Darawany, E. L., Fadiel, A. and Abdel Hafez, M. A. M. (2007). Physiological traits as affected by heat stress in sheep a review. Sm. Rumi. Res., 71: 1-12.
- Marcos-Carcavilla, A., Mutikainen, M., González, C., Jorge, H., Calvo, J., Kantanen, A., Nurbiy, S., Marzanov-María, D. and Magdalena Serrano, J.B. (2010). A SNP in the HSP90AA1 gene 52 flanking region is associated with the adaptation to differential thermal conditions in the ovine species. Cell Stress and Chaperones., 15:67–81.
- McManus, C., Paiva, S. R., Braccini, N.J., Barcellos, J.O., Dallago, B.S. (2013). Adaptations of Cattle to Stressful Environments. In: George Liu. (Org.). Cattle: Domestication, Diseases and the Environment. 1ead. Nova Publishers., 139-158.
- Nardone, A., Ronchi, B., Lacetera, N., Ranieri, M. S. and Bernabucci, U. (2010). Effect of climate changes on animal production and sustainability of livestock systems. Livestock Science., 130: 57–69.
- Paula-Lopes, F.F., Chase, J.R., Al-Katanani, C.C., Krininger, Y.M., Rivera, C.E., Tekin, R.M., Majewski, S., Ocon, A.C., Olson, T.A. and Hansen, P.J. (2003). Genetic divergence in cellular resistance to heat shock in cattle: differences between breeds developed in temperate versus hot climates in responses of preimplantation embryos, reproductive tract tissues and lymphocytes to increased culture temperatures. Anim. Reprod. Sci., 125:285–294.
- Sailo, L., Gupta, I.D., Verma, A., Singh, A., Chaudhari, M.V., Das, R., Upadhyay, R.C. and Goswami, J. (2015). Single nucleotide polymorphism in HSP90AB1 Gene and its association with thermo-tolerance in jersey crossbred cows. Anim. Sci. Report., 9: 43-49
- Sajjanar, B., Deb, R., Singh, U., Kumar, S., Brahmane, M., Nirmale, A., Kumar, S. and Minhas, P. (2015). Identification of SNP in HSP90AB1 and its Association with the Relative Thermo tolerance and Milk Production Traits in Indian Dairy Cattle. Anim. Biotech., 26: 921-928
- Sambrook, J. and Russell, D.W. (1989) Molecular cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
- Shergojry, A.S., Ramesha, K.P., Mir, A.N. and Aarif, O. (2011). Association of Single Nucleotide Polymorphisms (SNPS) Of HSP90AA1 Gene with Reproductive Traits in Deoni Cattle. Int. j. liv. Res., 1: 17-29
- Thom, E. C. (1959). The discomfort index. Weatherwise., 12: 57–59.
- Upadhyay, R. C., Sirohi, S., Ashutosh, S., Kumar, A. and Gupta, S. K. (2009). Impact of climate change on milk production in India. In: Global climate change and Indian agriculture (Edited by P. K. Aggarwal), Published by ICAR, New Delhi. pp: 104- 106.
- Upadhyay, R.C. (2010). 2% annual milk production loss due to global warming: research. Press trust of India/ New Delhi 26th September.
- Yeh, F. C., Yang, R. C. and Boyle, T. (1999). POPGENE VERSION 1.31: Microsoft Window-based free Software for Population Genetic Analysis, ftp://ftp.microsoft.com/Softlib/HPGL.EXE
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.