Indian Journal of Animal Research

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Research Article

Indian Journal of Animal Research, volume 51 issue 1 (february 2017) : 52-57

Cellular reprogramming of adult goat fibroblast: Toward pluripotency

Dharmendra Kumar, Rakesh Ranjan, Ajit P. Singh, Bikash C Sarkhel*
1<p>Animal Biotechnology Centre,&nbsp;Nanaji Deshmukh Veterinary Science University, Jabalpur-482 001, India.</p>
Cite article:- Kumar Dharmendra, Ranjan Rakesh, Singh P. Ajit, Sarkhel* C Bikash (2016). Cellular reprogramming of adult goat fibroblast: Toward pluripotency . Indian Journal of Animal Research. 51(1): 52-57. doi: 10.18805/ijar.10274.

ABSTRACT

Cellular reprogramming erases the epigenetic constraints of somatic cells genome and thus considered as key factor for success of somatic cell nuclear transfer technology. To achieve the reprogramming, different strategies are used which are mostly based on arresting the cell cycle at G0 or G1 stage. The present study was based on molecular investigation of reprogrammed cells for expression of pluripotent genes that are crucial for development of cloned embryos. The fibroblast cell lines were treated by four methods to induce cellular reprogramming viz., serum starvation, Roscovitin, aphidicolin and overconfluent. These treated cell lines were used for quantification of pluripotent gene transcripts by using real time PCR machine. The results showed that the relative expression of different pluripotent genes as Oct-4 and Nanog along with DNA methyl transferase gene (Dnmt-1) was observed in four treated cells. In case of normal cells, only Dnmt-1 gene was expressed, but pluripotent genes were not expressed at detection level. The expression of pluripotent genes in the donor cells prior to nuclear transfer have significant impact on cloning as because it facilitates the expression of that gene in the resulting embryo after nuclear transfer. The finding of this study may be extended for stem cell generation as it showed that pluripotent genes could be induced in the somatic cells without any transgenic incorporation.   

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