Indian Journal of Animal Research

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Research Article

Indian Journal of Animal Research, volume 50 issue 6 (december 2016) : 877-880

Genetic characterization of dahlem red layers by PCR-RAPD

Ch. Shivaprasad*1, B. Rarmesh Gupta2, R.N. Chatterjee3
1<p>Department of Animal Genetics and Breeding,&nbsp;College of &nbsp;Veterinary Science, Rajendranagar, Hyderabad-500 030, India.</p>
Cite article:- Shivaprasad*1 Ch., Gupta2 Rarmesh B., Chatterjee3 R.N. (2016). Genetic characterization of dahlem red layers by PCR-RAPD . Indian Journal of Animal Research. 50(6): 877-880. doi: 10.18805/ijar.11322.

ABSTRACT

A total of one hundred Randomly Amplified Polymorphic DNA (RAPD) markers were utilized to detect the DNA polymorphisms in a total of 48 full sib mated, half sib mated and non-in bred groups of Dahlem Red birds. Twenty one per cent of the primers tested yielded distinct polymorphic RAPD profiles. Out of 341 amplified bands 204 bands (59.82 %) were found to be polymorphic. The genetic similarity ranged from 83.94 to 87.90 % and, 81.14 to 85.25 % with in group based on band sharing and frequency respectively. Full sib group showed higher genetic similarity then non-in bred group. The genetic similarity based on band frequency ranged from 88.07 to 90.18 %, based on band frequency varied from 72.78 to 79.53 percent. The full sib and half sib groups showed maximum genetic similarity. The genetic distance between full sib and non-inbred groups was found to be the maximum (0.3176), while it was minimum between full sib and half sib groups.

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