Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

  • Print ISSN 0367-6722

  • Online ISSN 0976-0555

  • NAAS Rating 6.50

  • SJR 0.263

  • Impact Factor 0.4 (2024)

Frequency :
Monthly (January, February, March, April, May, June, July, August, September, October, November and December)
Indexing Services :
Science Citation Index Expanded, BIOSIS Preview, ISI Citation Index, Biological Abstracts, Scopus, AGRICOLA, Google Scholar, CrossRef, CAB Abstracting Journals, Chemical Abstracts, Indian Science Abstracts, EBSCO Indexing Services, Index Copernicus
Submit

Research Article

Indian Journal of Animal Research, volume 50 issue 6 (december 2016) : 846-850

Comparison of culture media for their effects on development of caprine IVF embryos using fresh and cryopreserved semen

Ajay Pratap Singh, Dharmendra Kumar, Gopalakrishna R, Rakesh Ranjan, Saurabh K Pandey, Bikash C Sarkhel*
1<p>Animal Biotechnology Centre, Nanaji Deshmukh Veterinary Science University, Jabalpur-482 001, India.</p>
Cite article:- Singh Pratap Ajay, Kumar Dharmendra, R Gopalakrishna, Ranjan Rakesh, Pandey K Saurabh, Sarkhel* C Bikash (2015). Comparison of culture media for their effects on development of caprine IVF embryos using fresh and cryopreserved semen . Indian Journal of Animal Research. 50(6): 846-850. doi: 10.18805/ijar.5709.

ABSTRACT

The aim of the present study was to compare the effects of two culture media on in vitro fertilized embryo produced by fresh and cryopreserved buck semen respectively. The in vitro fertilized embryos from both semen sources were cultured separately in the two media groups viz., Research Vitro Cleave-BSA (RVCL-BSA) and RVCL-Blast-BSA media. Under RVCL–BSA medium, the fresh semen produced higher rates of cleavage (68.94±1.86 vs 65.62±2.34) and significantly higher (Pd”0.05) morula (27.89±1.18 vs 23.84±2.23), blastocysts (15.26±0.67 vs 13.07±1.17) and blastomeric counts (232.65±14.26 vs 218.34 ± 10.26) than cryopreserved semen. The similar developmental patterns were exhibited in RVCL-Blast-BSA medium in which the fresh semen showed significantly higher (P0.05) rates of morula (38.94 ± 2.37 vs 31.87±2.10), blastocysts (29.89 ± 2.02 vs 25.31 ± 1.78) and blastomeric count (272.45 ± 16.54 vs 248.85 ± 14.38). This study concludes that the RVCL-Blast-BSA medium produced significantly higher (P0.05) embryonic developmental rates in comparison to RVCL– BSA medium irrespective of semen sources and the fresh semen afforded better fertilization rates than cryopreserved semen in both culture groups.

REFERENCES


  1. Bispo, C.A.S., G. Pugliesi., P. Galvao., M.T. Rodrigues., P.G. Ker., B. Filgueiras. and G.R. Carvalho. (2011). Effect of low and high egg yolk concentrations in the same extender for goat semen cryopreservation. Small Ruminant Research. 100: 54-58.

  2. Chatot, C.L., C.A. Ziomek., B.D. Bavister., J.L. Lewis. and I. Torres. (1989). An improved culture medium supports development of random-bred 1- cell mouse embryos in vitro. J Reprod Fertil. 86: 679-688.

  3. Cognie, Y., N. Poulin., P. Pignon., J. Sulon., J.F. Beckers. and Y. Guerin. (1995). Does heparin affect developmental ability of IVP goat oocytes? Proc 11th Meeting AETE, 146 (abstract).

  4. Colas, C., C. Junquera., R. Perez-Pe., J.A. Cebrian-Perez. and T. Muino-Blanco. (2009). Ultrastructural study of the ability of seminal plasma proteins to protect ram spermatozoa against cold-shock. Microsc Res Tech. 72: 566-572.

  5. Dorado, J., A. Munoz-Serrano. and M. Hidalgo. (2010). The effect of cryopreservation on goat semen characteristics related to sperm freezability. Animal Reproduction Science. 121: 115-123. 

  6. Fleming, T.P., W.Y. Kwong., R. Porter., E. Ursell., I. Fesenko., A. Wilkins., D.J. Miller., A.J. Watkins. and J.J. Eckert. (2004). The embryo and its future. Biol Reprod. 71: 1046-1054.

  7. Gopalakrishna, R., D. Kumar., A.P. Singh., S.K. Pandey., R. Ranjan. and B.C. Sarkhel. (2014). A Technique of Ooplasm Replenishment for Nuclear Transfer: Comparison of Embryo Quality with Conventional Nuclear Transfer Method. In Vitro Cellular and Developmental Biology-Animal. DOI 10.1007/s11626-014-9744-3.

  8. Gordon, I. (2003) Laboratory production of cattle embryos, In: Biotechnology in agriculture series, 2nd Edition, CAB International United Kingdom, 27.

  9. Jena, M.K., D. Malakar., A.K. De., S. Garg, , Y.S. Akshey., R. Dutta., S. Sahu., A.K. Mohanty. and J.K. Kaushik. (2012). Handmade cloned and parthenogenetic goat embryos-A comparison of different culture media and donor cells. Small Ruminant Research. 105: 255-262.

  10. Katska-Ksiazkiewicz, L., B. Rynska., B. Gajdaand. and Z. Smorag. (2004). Effect of donor stimulation, frozen semen and heparin treatment on the efficiency of in vitro embryo production of goats. Theriogenology. 62: 576-586.

  11. Kharche, S.D., A.K. Goel., S.K. Jindal., P. Yadav., R. Sinha. and N.K. Sinha. (2009). Effect of serum albumin supplementation on in- vitro capacitaion and fertilization of caprine oocytes. Small Ruminant Research. 81: 85-89.

  12. Kharche, S.D., P. Goel., P.K. Jha., A.K. Goel. and S.K. Jindal. (2011). Factors influencing in-vitro embryo production efficiency of caprine oocytes : A review. Indian Journal of Animal Sciences. 81: 344-61. 

  13. Kumar, D., R. Gopalkrishna., A.P. Singh., R. Ranjan., S.K. Pandey and B.C. Sarkhel. (2014). Developmental potency of pre-    implant parthenogenetic goat embryos : effect of activation protocols and culture media. In Vitro Cell. Dev. Biol.—    Animal. 50: 1-6.

  14. Lane, M. and D.K. (2007). Embryo culture medium: which is the best? Best Practice & Research Clinical Obstetrics and Gynecology. 21: 83-100.

  15. Leboeuf, B., B. Restall. and S. Salamon. (2000). Production and storage of goat semen for artificial insemination. Animal Reproduction Science. 62: 113-141.

  16. Medeiros, C.M.O., F. Forell., A.T.D. Oliveira. and J.L. Rodrigues. (2002). Current status of sperm cryopreservation: Why isn’t it better? Theriogenology. 57: 327-344.

  17. Mendes, Jr. J.O.B., P.D. Burns., J.F. De La Torre-Sanchez. and Jr. G.E. Seidel. (2003). Effect of heparin on cleavage rates and embryo production with four bovine sperm preparation protocols. Theriogenology. 60: 331-340.

  18. Ongeri, E.M., C.L. Borman., R.E. Butler., D. Melican., W.G. Gavin, and Y. Echelard. (2001). Development of goat embryos after in vitro fertilization and parthenogenetic activation by different methods. Theriogenology: 55: 933-945.

  19. Pellicer, M.T. (1995). Purificacion y caracterizacion del componente de la secrecion bulbouretral de macho cabrio implicado en el deterioro de la calida de los espermatozoides diluidos en leche. In: Tesina de Licenciatura (Universidad de Murcia). 200.

  20. Rodojcic, L., V. Vukotic-Maletic., and B. Balint., (1998). Current Knowledge on cryopreservation of spermatozoa, ovum cells and zygotes. Medicinski pregled. 51: 29.

  21. Romao, R., C.C. Marques., M.C. Baptista., M.I. Vasques., J.P. Barbas., A.E. M. Horta., N. Carolino., C. Bettencourt Plancha., P. Rodrigues. and R.M. Pereira. (2013). Evaluation of two methods of in vitro production of ovine embryos using fresh or cryopreserved semen. Small Ruminant Research. 110: 36-41.

  22. Shah, R.A., A. George., M.K. Singh., D. Kumar., M.S. Chauhan., R. Manik., P. Palta. and S.K. Singla. (2008). Handmade cloned buffalo embryos: comparison of different media and culture systems. Cloning Stem cells. 10: 435-441.

  23. Summers, M.C. and J.D. Biggers. (2003). Chemically defined media and the culture of mammalian preimplantation embryos: historical perspective and current issues. Hum. Reprod. 9: 557-558.

  24. Watson, P.F. (2000). The causes of reduced fertility with cryopreserved semen. Animal Reproduction Science. 60-61: 481-492.
Reviewed By

Download Article
In this Article
    Contact us
    Follow us

    Editorial Board

    View all (0)