Characterization and antibiogram of enteropathogenic Escherichia coli isolated from diarrhoeic and non-diarrhoeic dogs in South Bengal, India

DOI: 10.18805/ijar.5713    | Article Id: B-2951 | Page : 773-775
Citation :- Characterization and antibiogram of enteropathogenic Escherichia coli isolated from diarrhoeic and non-diarrhoeic dogs in South Bengal, India .Indian Journal Of Animal Research.2016.(50):773-775

A. Banik, D.P. Isore, S.N. Joardar, K. Batabyal* and S. Dey

drkb.micro@gmail.com
Address :

Department of Veterinary Microbiology, West Bengal University of Animal and Fishery Sciences, Kolkata–700 037, India

Submitted Date : 16-02-2015
Accepted Date : 11-06-2015

Abstract

Diarrhoea in canines is mainly caused by Escherichia coli which can be fatal also. To understand the depth of this infection, a study was undertaken to detect E. coli isolates from diarrhoeic and non-diarrhoeic dogs in Southern part of West Bengal. A total of 112 canine samples were tested during May to September 2012 revealing approx 63.4% (71) samples positive for Escherichia coli. The most common serotypes were O8 (23.9%) followed by O157 (19.7%), O101 (16.9%), O26 (15.5%), O153 (12.7%) and O6 (11.3%). Among these O6, O8, O26 and O157 were highly pathogenic to mice causing almost 100% mortality within 24hrs of inoculation and were also detected to be haemolytic on sheep blood agar plates except serotype O8. These isolates were mostly sensitive to nalidixic acid (80.29%), cotrimoxazole (78.88%), ciprofloxacin (74.65%), colistin and ceftriaxone (both 71.83%) but were resistant to amikacin (97.18%), kanamycin (95.78%), cephalexin (92.96%) and enrofloxacin (84.51%).

Keywords

Antibiogram Diarrhoea Dogs Escherichia coli Pathogenicity.

References

  1. Baines, D., Erb, S., Turkington, K., Kuldau, G., Juba, J., Sumarah, M., Mazza, A. and Masson, L. (2011). Characterization of haemorrhagic enteritis in dairy goats and the effectiveness of probiotic and prebiotic applications in alleviating morbidity and production losses. Fungal Genom. Biol., 1: 102-109.
  2. Bauer, A.W., Kirby, W.M.M., Sherris, J.C. and Truck, M. (1966). Antibiotic sensitivity test by a standardized disc method. Am. J. Clin. Path., 45: 493-496.
  3. Beutin, L. (1999). Escherichia coli as a pathogen in dogs and cats. Vet. Res., 30: 285-298.
  4. Beutin, L., Geier, D., Steinrück, H., Zimmermann, S. and Scheutz, F. (1993). Prevalence and some properties of verotoxin (Shiga-like toxin)-producing Escherichia coli in seven different species of healthy domestic animals. J. Clin. Microbiol., 31: 2483-2488.
  5. Buxton, A. and Fraser, G., (1977). Animal Microbiology, Vol-I, Blackwell Scientific Publications, Oxford, London, Edinburg, Melbourne, pp. 94-100.
  6. Chakraborty, D. and Nag, N.C. (1998). Some observations on the colicinogenicity and enteropathogenicity of Escherichia coli associated with gastroenteritis in calves. Indian J. Anim. Hlth., 37: 17-20. 
  7. Chattopadhyay, U.K., Gupta, S. and Dutta, S. (2003). Search for Shiga toxin producing Escherichia coli (STEC) including O157:H7 strains in and around Kolkata. Indian J. Med. Microbiol., 21: 17-20.
  8. Cortes, C., De la Fuente, R., Blanco, J., Blanco, M., Blanco, J.E., Dhabi, G., Mora, A., Justel, P., Contreras, A., Sanchez, A., Corrales, J.C. and Orden, J.A. (2005). Serotyping, virulence genes and intimin types of verotoxin-producing Escherichia coli and enteropathogenic E. coli isolated from healthy dairy goats in Spain. Vet. Microbiol., 110: 67-76.
  9. Hammermueller, J., Kruth, S. and Prescott, J.(1995). Detection of toxin genes in Escherichia coli isolated from normal dogs and dogs with diarrhoea. Can. J. Vet. Res., 59: 265-270. 
  10. Hariharan, H., Mada, C., Poole, D. and Page, R. (2004). Antibiotic resistance among enterotoxigenic Escherichia coli from piglets and calves with diarrhea. Can. Vet. J., 45: 605-606.
  11. Joon, D.S. and Kaura, Y.K. (1993). Isolation and characterization of some of enterotobacteria from diarrhoeic and non-    diarrhoeic calves. Indian J. Anim. Scs., 63: 373-383.
  12. Manna, K., Batabyal, K. and Das, R. (2005). Studies on haemolytic characters and pathogenicity of Escherichia coli isolated from dogs. Indian Vet. J., 82: 216-217.
  13. Marques, S.B.S. and Marin, J.M. (2007). Susceptibility of newborn mice to intestinal colonization by Enteropathogenic Escherichia coli isolated from milk of Bovine with Mastitis. Ars. Veterinaria, 23: 95-99.
  14. Rekha, B.S. and Krishnappa, G. (2001). Bacterial Flora in Canine Pyometra. Indian Vet. J., 78: 773-774. 
  15. Rodrigues, J., Thomazini, C.M., Lopes, C. A. M. and. Dantas, L. O. (2004). Concurrent Infection in a Dog and Colonization in a Child with a Human Enteropathogenic Escherichia coli Clone. J. Clin. Microbiol., 42: 1388-1389.
  16. Salvadori, M.R., Valadares, G.F., Leite, D.S., Blanco, J. and Yano, T. (2003). Virulence factors of Escherichia coli isolated from calves with diarrhea in Brazil. Brazilian J. Microbiol., 34: 230-235.
  17. Sancak, A.A., Rutgers, H.C., Hart, C.A. and Batt, R.M. (2004). Prevalence of enteropathic Escherichia coli in dogs with acute and chronic diarrhoea. Vet. Rec., 154: 101-106.
  18. Sanyal, D., Douglas, T. and Roberts, R. (1997). Salmonella infection acquired from reptilian pets. Arch. Dis. Child., 77: 345-346.
     

Global Footprints