Detection of polymorphism of Calgranulin A gene in Indian Murrah Buffalo

DOI: 10.18805/ijar.5715    | Article Id: B-2946 | Page : 658-661
Citation :- Detection of polymorphism of Calgranulin A gene in Indian Murrah Buffalo .Indian Journal Of Animal Research.2016.(50):658-661

Sourabh Sulabh*, Archana Verma, I.D. Gupta and S. Rajesh Kumar

sourabhjsr@yahoo.com
Address :

Dairy Cattle Breeding Division, ICAR-National Dairy Research Institute, Karnal-132 001, India.

Submitted Date : 13-02-2015
Accepted Date : 2-04-2015

Abstract

Calgranulin A (S100A8) gene is one of the important candidate genes, which affects the host disease resistance by enhancing the immune system. Present study was undertaken with the objectives to identify polymorphism in Calgranulin A gene and to associate identified genetic variants with the incidence of clinical mastitis in Murrah buffalo. Genomic DNA was isolated from 100 randomly selected lactating Murrah. Two sets of primers were designed to amplify targeted regions of the gene. PCR products were obtained at annealing temperature of 63.8oC and were of 449 and 489 bp for respective primer set. PCR-RFLP analysis was carried out using HinfI for contig I and AluI and MboII restriction endonucleases for contig II. Both the contigs revealed monomorphism with frequency of the only prevailing A allele as 1.00. It was not feasible to analyze association with the incidence of mastitis, as no genetic variants were observed in the animals studied.

Keywords

Calgranulin A Mastitis Murrah buffalo PCR-RFLP S100A8.

References

  1. Ceron-Munoz, M., Tonhati, H., Duarte, J., Oliveira, J., Munoz-Berrocal, M. and Jurado-Gamez, H. (2002). Factors affecting somatic cell counts and their relations with milk and milk constituent yield in buffaloes. J. Dairy Sci. 85: 2885–2889.
  2. Dua, K. (2001). Incidence, etiology and estimated economic losses due to mastitis in Punjab and in India - An update. Indian Dairyman. 53: 41–52. 
  3. Foell, D., Wittkowski, H., Vogl, T., and Roth, J. (2007). S100 proteins expressed in phagocytes: a novel group of damage-    associated molecular pattern molecules. Journal of Leukocyte Biology. 81 : 28-37
  4. Kathiravan, P., Goyal, S., Kataria, R. S., Mishra, B. P., Jayakumar, S. and Joshi, B. K. (2011). Sequence characterization of S100A8 gene reveals structural differences of protein and transcriptional factor binding sites in water buffalo and yak. Animal Biotechnology. 22: 124-132
  5. Nivsarkar, A. E., Vij, P. K. and Tantia, M. S. (2000). Animal Genetic resources of India cattle and buffalo, Indian Council of Agricultural Research, New Delhi, edited by Sharma, R. P. ICAR, pp. 144–149.
  6. Ogorevc, J., Kunej, T. and Dovè, P. (2008). An integrated map of cattle candidate genes for mastitis: a step forward to new genetic markers. Acta Agri. Slovenica. 2:85–91.
  7. Ogorevc, J., Kunej, T., Razpet, A. and Dovè, P. (2009). Database of cattle candidate genes and genetic markers for milk production and mastitis. Anim. Genet. 40: 832-851.
  8. Rainard, P. and Riollet, C. (2006). Innate immunity of the bovine mammary gland. Veterinary Research. 37: 369-400.
  9. Sambrook, J. and Russell, D. W. (2001). Preparation and analysis of eukaryotic DNA. In: Molecular Cloning: A Laboratory Manual. 3rd Edition. Cold Spring Harbor Laboratory Press, New York: 6.1-6.62.
  10. Shang, X., Cheng, H. and Zhou, R. (2008). Chromosomal mapping,differential origin and evolution of the S100 gene family. Genet.Set.Evol. 40: 449-464.
  11. Thirunavukkarasu, M., Kathiravan, G., Kalaikannan, A. and Jebarani, W. (2010). Quantifying economic losses due to milk fever in dairy farms. Agricultural Economics Research Review . 23 :77-81
  12. Thiruvenkadan, A. K., Rajendran, R.and Muralidharan, J. (2013). Buffalo genetics resources of India and their conservation. Buffalo Bull. 32: 227–235

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