Indian Journal of Animal Research

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Research Article

Indian Journal of Animal Research, volume 49 issue 6 (december 2015) : 814-818

G and P types of bovine group A rotavirus in northern India

Tripti Singh, Rashmi singh*1, Ajay Pratap Singh1, Y.P.S. Malik2, Minakshi Prasad3
1<p>College of Biotechnology, U.P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa VigyanVishwavidyalya&nbsp;<br /> evam Go-Anusandhan Sansthan, Mathura-281 001, Uttar Pradesh, India.</p>
Cite article:- Singh Tripti, singh*1 Rashmi, Singh1 Pratap Ajay, Malik2 Y.P.S., Prasad3 Minakshi (2015). G and P types of bovine group A rotavirus in northern India . Indian Journal of Animal Research. 49(6): 814-818. doi: 10.18805/ijar.7044.

ABSTRACT

Animal rotaviruses (RVs) are considered as a potential threat to humans due to possibility of interspecies transmission and exchange of genomic materials. Among several RVs, the group A rotaviruses (RVA) are the major cause of diarrhoea in cattle and buffalo calves worldwide. The present study was carried out to understand epidemiology and types of RVA circulating in Mathura region of northern India. One hundred faecal samples were collected from diarrhoeic cattle (n=94) and buffalo calves (n=6) from organized dairy farms. Viral RNA was extracted from faecal suspension and was transcribed to cDNA using RT-PCR. The cDNA was amplified for VP6, VP7 and VP4 genes and multiplex nested PCR was done for G and P genotyping. Twelve samples were detected positive for RVA by antigen detection ELISA and eleven samples produced expected amplicon for group A specific VP6 gene. On genotyping with G3, G6, G8 and G10 specific primers for VP7 gene and P [1] and P [11] specific primers for VP4 gene, G6 genotype predominated over other genotypes. Dual genotypes were also observed. In P typing only one isolate was found to be positive for P [1] and rest all samples remained untypable. The result of present study indicates G6 as a major G genotype and change in frequencies of distribution of G types in this part of the country. 

REFERENCES


  1. Basera, S.S.; Singh, R.; Vaid, N.; Sharma, K.; Chakravarti, S. and Malik, Y.P.S. (2010). Detection of rotavirus infection in bovine calves by RNA-PAGE and RT-PCR. Indian J. Virol.21: 144-147.

  2. Chitambar, S.D.; Arora, R.; Kolpe, A.B.; Yadav, M.M. and Raut, C.G. (2011). Molecular characterization of unusual bovine group A rotavirus G8P [14] strains identified in western India: Emergence of P[14] genotype. Vet. Microbiol. 148: 384-388.

  3. Dash, S.K.; Tewari, A.; Kumar, K.; Goel, A. and Bhatia, A.K. (2011). Detection of Rotavirus from diarrhoeic cow calves in Mathura, India Vet. World. 4: 554-556.

  4. Ezung, Z.N.; Singh, R.; Singh, S.P.; Kumar, N. and Malik, Y.P.S. (2014). Occurrence of multiple combinations of G and P types of group A bovine and human rotaviruses in Uttarakhand and Nagaland states, India. Indian J. Anim. Sci. 84 : 851-854.

  5. Ghosh, S.; Varghese, V.; Samajdar, S.; Sinha, M.; Kobayashi, N. and Naik, T.N. (2007a). Molecular characterization of bovine group A rotavirus G3P [3] strains. Arch. Virol.152: 1935-1940.

  6. Ghosh, S.; Varghese,V.; Sinha,M.; Kobayashi,N. and Naik,T.N.; (2007b). Evidence for interstate transmission and increase in prevalence of bovine group B rotavirus strains with a novel VP7 genotype among diarrhoeic calves in Eastern and Northern states of India. Epidemiol. Infect. 135: 1324-1330.

  7. Ghosh, S.; Samajdar, S.; Sinha, M.; Kobayashi, N.; Taniguchi, K. and Naik, T. N. (2008). Molecular characterization of rare bovine group A rotavirus G15P [11] and G15P [21] strains from eastern India: identification of simian SA11-    like VP6 genes in G15P [21] strains. Virus Genes.37: 241-249. 

  8. Gulati, B.R.; Nakagomi, O.; Koshimura, Y.; Nakagomi, T. and Pandey, R. (1999). Relative frequencies of G and P types among rotaviruses from Indian diarrheic cow and buffalo calves. J. Clin. Microbiol. 37: 2074–2076.

  9. Iturriza-Gómara M, Kang G and Gray J. (2004). Rotavirus genotyping: keeping up with an evolving population of human rotaviruses. J. Clin. Virol.31: 259-65.

  10. Malik, Y.P.S.; Sharma, K.; Vaid, N.; Chakravarti, S.; Chandrashekar, K.M.; Basera, S.S.; Singh, R.; Minakshi; Prasad, G.; Gulati, B.R.; Bhlegaonkar, K.N. and Pandey, A.B. (2012). Frequency of group A rotavirus with mixed G and P genotypes in bovines: predominance of G3 genotype and its emergence in combination with G8/G10 types. J. Vet. Sci. 13: 271-278.

  11. Malik, Y. P. S.; Sharma, K.;Vaid, N.;Batra, M.; Prasad, M.;Prasad, G.; Gulati, B. R. and Bhilegaonkar, K. N. (2013). Designing and validation of new primers for efficient genotyping of animal G3 rotaviruses. Vet. Arhiv.83: 611-22.

  12. Manuja, B.K.; Prasad, M.; Manuja, A.; Gulati, B.R. and Prasad, G. (2008). A novel genomic constellation (G10P[3]) of group A rotavirus detected from buffalo calves in northern India. Virus Res. 138: 36-42.

  13. Minakshi; Prasad, G.; Malik, Y.P.S. and Pandey, R. (2005). G and P genotyping of bovine group A rotavirus in faecal samples of diarrhoeic calves by DIG-labelled probes. Indian J. Biotech. 4: 93-99.

  14. Minakshi; Prasad, G. and Grover, Y.P. (2009). Occurrence of dual infection of bovine group A rotavirus in diarrhoeic calf in Haryana, India. Indian J. Anim. Sci. 79: 1205-1208.

  15. Mondal, A.; Majee, S.B. and Bannalikar, A.S. (2011). Molecular characterization of vp7 gene of bovine group A rotavirus. Anim. Sci. Rep. 5: 27-33.

  16. Mondal, A.; Aich, R.; Majee, S. and Bannalikar, A.S. (2012). Determination of bovine rotavirus G serotype by polymerase chain reaction. Trop. Anim. Health Prod. 44: 763–767.

  17. Monini, M.; Cappuccini, F.; Battista, P.; Falcone, E.; Lavazza, A. and Ruggeri, F.M. (2008). Molecular characterization of bovine rotavirus strains circulating in northern Italy, 2003–2005. Vet. Microbiol. 129: 384–389. 

  18. Nataraju, S.M.; Chattopadhyay, U.K. and Krishnan, T. (2009). A study on the possibility of zoonotic infection in rotaviral diarrhoea among calves and buffalo calves in and around Kolkata, India. Europ. Review Med. Pharmacol. Sci. 13 : 7-11.

  19. Parwani, A.V.; Hussein, H.A.; Rosen, B.I.; Lucchelli, A.; Navarro, L. and Saif, L.J. (1993). Characterization of field strains of group A bovine rotaviruses by using polymerase chain reaction-generated G and P type-specific cDNA probes. J. Clin. Microbiol. 31: 2010-2015.

  20. Saravanan, M.; Parthiban, M. and Ramdass, P. (2006). Genotyping of rotavirus of neonatal calves by nested-multiplex PCR in India. Vet. Archiv. 76: 497–505.

  21. Singh, T.; Singh, R. and Singh, A.P. (2013). Detection and prevalence of bovine group A rotavirus in Mathura region. J. Immunol. Immunopathol. 15 : 175-180.

  22. Suresh, T.; Rai, R.B.; Dhama, K.; Sawant, P.M.; Kumar, D. and Bhatt, P. (2012). Determination of G and P type diversity of group A rotaviruses and detection of a new genotype from diarrhoeic calves in northern and southern states of India. Vet. Pract. 13: 1-8.

  23. Tewari, A.; Dash, S.K.; Jain, B. and Bhatia, A.K. (2012). Detection of P1 type of bovine rotavirus using nested multiplex PCR in Mathura, India. J. Anim. Res. 2: 179-185. 

  24. Varshney, B.; Jagannath, M.R.; Vethanayagam, R.R.; Kodhandharaman, S.; Jagannath, H.V.; Gowda, K.; Singh, D.K. and Rao, C.D. (2002). Prevalence of and antigenic variation in serotype G10 rotaviruses and detection of serotype G3 strains in diarrheic calves: implications for the origin of G10P11 or P11 type reassortant asymptomatic strains in newborn children in India. Archiv. Virol. 147: 143–165.

  25. Wani, S.A.; Bhat, M.A.; Ishaq, S.M. and Ashrafi, M.A. (2004). Determination of bovine rotavirus G genotypes in Kashmir, India. Rev. Sci. Tech. 23: 931-936.



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