Determination of pre and post treatment oxidative status and oxidativeDNA damage in diarrheic calves

ABSTRACT

Increase in production of free radicals causes damage in lipids of cell membrane, weakening in functions of cell proteins and DNA damage. The aim of the present study was to determine the oxidative status, level of oxidative DNA damage and treatment effectiveness on these parameters on diarrheic calves. The study was conducted using 10 clinically holstein calves from 2-3 months of age. Antidiarrheic treatment containing Amonium sulphate (30mg/kg/day, Gabbrocol, Ceva Inc.), a mixture of Bismuth subcarbonate, kaolin, pectin (10g/day, Bismol, Bioteknik Inc.), vitamin and mineral mixture (105g/day, Sky High Energy, Egevet Co.Ltd) as drugs orally administered to all calves for 3 days. Body temperature, feces and blood samples were taken before and after treatment. Dry matter measured (%) in feces, DNA damage level and some hematologic parameters, total oxidant status (TOS), total antioxidant status (TAS) and some biochemical parameters were measured from blood samples. HB, ALT, TP levels did not differ before and after treatment. However, body temperature (p<0.01), WBC (p<0.01), HCT (p<0.05), GGT (p<0.01), TOS (p<0.05), DNA damage (p<0.05) were decreased, dry matter in feces (p<0.01), RBC (p<0.05), AST (p<0.01), Albumin (p<0.01) and TAS (p<0.05) were increased after treatment. The results of the study indicate that oxidative DNA damage increases with degenerative diseases such as diarrhea and decreases to the normal range after effective treatment. It can be suggested from these results that oxidative DNA damage might be a good indicator to reveal the degeneration level of diarrhea in animals and a good parameter to evaluate the effectiveness of the treatment in terms of cellular form.

KEYWORDS

REFERENCES

Abe M, Miyajima, Y, Hara, T, Wada, Y, Funaba M and Iriki, T (1999). Factors affecting water balance and fecal moisture content in suckling calves given dry feed. J Dairy Sci. 82: 1960–1967

Alle, RG and Tresini M (2000). Oxidative stress and gene regulation. Free Rad Biol Med. 28: 463–499

Altindag O, Kocyigit A, Çelik N, Çelik H and Soran N (2007). DNA Damage and oxidative stress in patients with osteoarthritis: a pilot study. Rheumatism. 22: 60-63

Aslan M, Nazligu Y, Bolukbas C, Bolukbas FF, Horoz M, Dulger AC, Erdur FM, Celik H and Kocyigit A (2011). Peripheral lymphocyte DNA damage and oxidative stress in patients with ulcerative colitis. Pol Arch Med Wewn. 121: 223-9

Blanchard PC (2012). Diagnostics of dairy and beef cattle diarrhea. Vet Clin North Am Food Anim Pract. 28: 443-64

Block G, Patterson B and Subar A (1992). Fruit, vegetables and cancer prevention: a review of the epidemiological evidence. Nutr Cancer. 18: 1–29

Cigerci IH, Fidan F, Konuk M, Yuksel H, Kucukkurt I, Eryavuz A and Sozbilir NB (2009). The protective potential of Yuccaschidigera (Sarsaponin 30) against nitrite-induced oxidative stress in rats. J Nat Med. 63: 311–317

Cleek JL and Phillips RW (1981). Evaluation of a commercial preparation for oral therapy of diarrhea in neonatal calves: administration by suckling versus intubation. JAVMA 178: 977-981

Coles EH (1986). Kidney Function, In Veterinary Clinical Pathology, 4th Ed, WB Saunders Company, Philadelphia, p 171- 200

Collins AR, Ai-guo M and Duthie SJ (1995). The kinetics of repair of oxidative DNA damage (strand breaks and oxidised pyrimidines) in human cells. Mutat. Res. 336: 69-77

Cooke MS, Olinski R and Evans MD (2006). Does measurement of oxidative damage to DNA have clinical significance. Clin Chim Acta. 365: 30-49

Davies KJ (1995). Oxidative stress: the paradox of aerobic life. Biochem Soc Symp 61: 1–31

Del Maestro RF (1980) An approach to free radicals in medicine and biology. Acta Physiol. 492: 153-168

Dundar Y and Aslan R (1999) Oksidan-Antioksidan denge ve korunmasýnda vitaminlerin rolü. Hay Araþt Enst Derg. 9: 32-39

Erel O (2004). A novel automated method to measure total antioxidant response against potent free radical reactions. Clin Biochem. 37: 112 119

Erel O (2005). A new automated colorimetric method for measuring total oxidant status. Clin Biochem. 38: 1103-1111

Hall GA, Jones PW and Morgan JH (1992). Calf diarhoea, chapter 12, in “Bovine Medicine Diseases and Husbandry of Cattle” Editors, AH Andrews, RW Blowey, H Boyd, RG Eddy, 1st ed. Blackwell Science Ltd.,Oxford

Heaton PR, Ransley R, Charlton CJ, Mann SJ, Stevenson J, Smith BHE, Rawlings JM and Harper EJ (2002). Application of single-cell gel electrophoresis (comet) assay for assessing levels of DNA damage in canine and feline leukocytes. J Nutr. 132: 1598–1603

Ho KJ, Chen PQ, Chang CY and Lu FJ (2000). The oxidative metabolism of circulating phagocytes in ankylosing spondylitis: determination by whole blood chemi luminescence. Ann Rheum Dis 59: 338-41

Karademir B and Sendil Ç (2001). Clinical, haemotological and biochemical findings of diarrhea caused by E.Coli and the efficancy of treatment applications calf. Kafkas Univ Vet Fak Derg. 7: 175-183

Karakucuk S, Baskol G, Oner AO, Baskol M, Mirza E and Ustdal M (2004). Serum paraoxonase activity is decreased in the active stage of Behcet’s disease. Br J Ophthalmol 88: 1256-8

Keli SO, Hertog GL, Feskens EJM and Kromhout D (1996). Dietary ûavanoids, antioxidant vitamins, and the incidence of stroke. Arch Int Med. 156: 637-42

Kucukkurt I, Cigerci IH, Ince S, Kozan E, Aytekin I, Eryavuz A and Fidan F (2014) The effects of babesiosis on oxidativestress and DNA damage in anatolian black goats naturally infected with babesia ovis. Iranian J Parasitol. 9: 90-98

Ocal N, Duru SY, Yagci BB and Gazyagci S (2006). Field condition diagnosis and treatment of acid-base balance disorders in calves with diarrhea. Kafkas Univ Vet Fak Derg. 12:175-183

Pekcan M, Altintas A, Karagul H, Fidanci UR, Uysal H and Besalti O (2012). Serum Biochemistry And Native Protein Electrophoresis In Diarrheic Calves With Arthritis. Acta Veterinaria (Beograd). 62: 261-269

Risalde MA, Molinaa V, Sánchez-Cordóna PJ, Pedreraa M, Panadero R, Romero-Palomoa F and Gómez-Villamandos JC (2011). Response of proinflammatory and anti-inflammatory cytokines in calves with subclinical bovine viral diarrhea challenged with bovine herpesvirus-1. Vet Immunol Immunopathol. 144:135-143

Saravan R and Pugalendi KV (2005). Assessment of the pharmacological effect of silymarin on ethanol induced DNA damage by sindle cell gel electrophoresis. Indian J Pharmacol. 37: 261–2

Serafini M and Del Rio D (2004). Understanding the association between dietary antioxidants, redox status and disease: is the total antioxidant capacity the right tool. Redox Report. 9: 145-152

Smith DR (2012). Field disease diagnostic investigation of neonatal calf diarrhea. Vet Clin North Am Food Anim Prac. 28: 465-81

Vermunt JJ 1994. Rearing and management of diarrhea in calves to weaning. Aust Vet. J 71: 33-41

Disclaimer :

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.

Copyright :

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Indian Journal of Animal Research

Determination of pre and post treatment oxidative status and oxidative DNA damage in diarrheic calves

ABSTRACT

KEYWORDS

REFERENCES

Reviewed By

In this Article

APC

Publish With US

Become a Reviewer/Member

Open Access

Products and Services

Support and Policies

Editorial Board