Effect of milking behavior on circulatory hormones and milk production in lactating Murrah buffaloes

DOI: 10.18805/ijar.8567    | Article Id: B-2922 | Page : 123-128
Citation :- Effect of milking behavior on circulatory hormones and milk productionin lactating Murrah buffaloes .Indian Journal Of Animal Research.2016.(50):123-128

Mahendra Singh*, Anjali Aggarwal and S. Mallick

chhokar.ms@gmail.com
Address :

Dairy Cattle Physiology Division, ICAR-National Dairy Research Institute, Karnal -132 001, India.

Submitted Date : 15-01-2015
Accepted Date : 8-09-2015

Abstract

The effect of milking temperament on plasma hormones, metabolites, milk yield and composition in lactating Murrah buffaloes was studied. Animals were selected from the institute herd and grouped as docile and nervous buffaloes, based on temperament score before and during milking. Blood sampling times were; before milking (-20 min.), after concentrate intake, after teat massage, during milking and 20 min. post- milking. Plasma prolactin (PRL), growth hormone (GH) and cortisol (CORT) concentrations were measured by enzyme-immunoassay procedures. Milk yields of individual buffaloes were recorded and milk composition (fat, protein, lactose, SNF, SCC) determined. Milk yield was significantly lower (P<0.05) in nervous buffaloes in comparison to docile buffaloes. Mean fat, protein, lactose and SNF varied non-significantly between the groups. Milk SCC and plasma NEFA concentration was more (P<0.05) in nervous buffaloes than in docile buffaloes. Milking temperament significantly influenced plasma PRL (P<0.01) and CORT release (P<0.05) but GH was unaffected.  Concentrate feeding prior to milking induced release of plasma PRL and CORT without affecting GH and oxytocin levels. Teat stimulus further enhanced release of PRL, oxytocin and CORT hormone in both the groups. Plasma PRL, cortisol, oxytocin and GH varied significantly (P<0.01) before, during and after milking (P<0.01). Basal PRL, GH and CORT were attained 20 minutes after the completion of milking in both the groups. The results show that the behavior of buffaloes at milking influenced release of  galactopoietic hormones (PRL, CORT, GH and oxytocin), milk yield and SCC, while milk fat, protein, lactose and SNF remain unaffected. 

Keywords

Concentrate feeding Hormone Metabolites Milking temperament Milk yield and composition Teat stimulus.

References

  1. Boselli, C.; Mazzi, M.; Borghese, A.; Terzano, G.M.; Giangolini, G.; Filippetti, F.; Amatiste, S. and Rosati, R. (2010). Milk flow curve and teat anatomy in Mediterranean Italian buffalo cows. Proceedings of the 9th World Buffalo Congress, Buenos Aires, Argentina, pp 573-578.
  2. Bruckmaier, R.M. and Blum, J.W. (1992). Ultrasonography of mammary glands of cows, goats and sheep during alpha- and beta-adrenergic agonist and oxytocin administration. J. Dairy Res., 59: 151–159. 
  3. Bruckmaier, R.M.; Schams, D. and Blum, J.W. (1992). Etiology of disturbed milk ejection in parturient primiparous cows. J. Dairy Res., 59:479-489. 
  4. Bruckmaier, R.M.; Schams, D. Blum J.W. (1993). Milk removal in familiar and unfamiliar surroundings: concentration of oxytocin, prolactin, cortisol, and b-endorphin. J. Dairy Sci., 60: 449-456. 
  5. Bruckmaier, R.M. and Blum, J.W. (1998). Oxytocin release and milk removal in ruminants. J. Dairy Sci., 81:939-949. 
  6. Cook, C.J. (1997). Oxytocin and prolactin suppress cortisol responses to acute stress in both lactating and non-lactating sheep. J. Dairy Res 64: 327-339. 
  7. Combellas, J. and Tesorero, M. ( 2003). Cow-calf relationship during milking and its effect on milk yield and calf live weight gain. Liv. Res. Rur. Dev., 15 :3.
  8. Curley Jr. K. O., Neuendorff, D. A. , Lewis, A. W. , Cleere, J. J., Welsh, T. H. and Randel, R. D. (2008). Functional characteristics of the bovine hypothalamic-pituitary-adrenal axis vary with temperament,” Hor. Beh., 53: 20–27.
  9. Gorewit, R.C.; Svennersten, K.; Butler, W.R. and Uvnäs-Moberg, K. (1992). Endocrine responses in cows milked by hand and machine. J. Dairy Sci., 75: 443-448. 
  10. Gupta, S.C.; Handa M.C. and Sahoo, C. (1985). Dairy temperament of buffaloes in relation to their milking ability. Indian J. Anim. Prod. Mgmt., 1:116.
  11. Kaskous, S.H.; Weiss, D.; Massri, Y.; Al-Daker, A.M.; Nouh, A.D. and Bruckmaier, R.M. (2006). Oxytocin release and lactation performance in Syrian Shami cattle milked with and without suckling. J. Dairy Res., 73: 28–32.
  12. Kasimanickam, R, Schroeder, S., Assay, M., Kasimanickam, V., Moore, D.A., Gay, J.M., Whittier, W.D. 2014. Influence of temperament score and handling facility on stress, reproductive hormone concentrations, and fixed time AI pregnancy rates in beef heifers. Reprod. Domest. Anim., 49:775-82.
  13. Knight, C.H. and Flint, D.J. (1995). GH: prolactin interactions in lactating rodents and ruminants. Hannah research Institute Yearbook. pp. 73-78. 
  14. Ludri, R.S. and Singh, M. (1987). Milk production, dry matter and water consumption of crossbred cows milked with and without oxytocin. Indian J. Anim. Sci., 57: 778-780. 
  15. Ludri, R.S.; Upadhyay, R.C.; Singh, M.; Guneratne, J.R. and Basson, R.P. (1989). Milk production in buffaloes receiving recombinantly produced bovine somatotropin (BST). J .Dairy Sci., 72:2283-2287. 
  16. Mepham, T.B. (1987). Physiology of lactation, Open University press, Philadelphia, USA. 
  17. Michelle, C.L. and Singh, M. (2008). Influence of hand and machine milking on incidence of cisternal and alveolar milk yield and compositional changes in Murrah buffaloes. Indian J. Dairy Sci., 61: 70-73. 
  18. Michelle, C.L. and Singh, M. (2009). Milking behavior of Murrah buffaloes during hand and machine milking. Indian Vet. J., 86: 171-173. 
  19. Naoshige, ABE.; Hiroyuki, HIROOKA.; Hirotoshi, TAKAZAKI. and Yoshimasa, KUBOTA. (2002). Possible Factors affecting the Milking Temperament of Holstein Cows. Nihon Chikusan Gakkaiho 73: 515-520. 
  20. Nayak, S. and M. Mishra. (1984). Dairy temperament of Red Sindhi, Crossbred and Murrah buffaloes in
  21. relation to their milking ability and composition. Indian J. Dairy Sci., 37:20-23.
  22. Negrao, J.A. (2008). Hormone release and behavior during suckling and milking in Gir, Gir x Holstein and Holstein cows. J. Anim. Sci.,86: 21-26. 
  23. Orihuela, A. (1990). Effect of calf stimulus on the milk yield of Zebu-type cattle. Applied Anim. Behavior Sci., 26: 187-190. 
  24. Perry, N.A. and Doon, R.J. (1950). Picric and method for simultaneous determination for lactose and sucrose in dairy products. J. Dairy Sci., 33: 176-180. 
  25. Prasad, J. and Singh, M. (2010). Milk production and hormonal changes in Murrah buffaloes administered recombinant Bovine Somatotropin (rBST). Agri. Biol. J. North America., 1: 1325-1327. 
  26. Ramasamy, M. and Singh, M. (2004). Postpartum milking behavior and its effect on milk production in 
  27. Murrah buffaloes (bubalus bubalis). Indian J. Anim. Sci., 74: 530-533. 
  28. Roy, P.K. and P.K. Nagpaul. (1984). Influence of genetic and non-genetic factors on temperament score and other traits of dairy management. Indian J. Anim Sci., 54:566-568.
  29. Sastry, N.S.R. and V.N. Tripathi. (1988). Modern management innovations for optimising buffalo production. Buffalo production and health: a compendium of latest research information based on Indian
  30. studies. 2nd World Buffalo Congress, New Delhi, India: 38-62.
  31. Shipe, W.F.; Senyk, G.F. and Fountain, K.B. (1980). Modified copper soap solvent extraction method for measuring free fatty acids in milk. J. Dairy Sci., 63: 193-198. 
  32. Singh, M. and Ludri, R.S. (1994). Plasma growth hormone profile and milk yield response of Murrah buffaloes (Bubalus bubalis) treated with slow release formulation of somidobove. Buffalo J. 10: 81- 84. 
  33. Singh, M. and Ludri, R.S. (2001). Somatic cell counts in Murrah buffaloes during different stages of lactation, parity and season. Asian Austr. J. Anim. Sci., 41: 189-192. 
  34. Singh, M. and Prakash, B. S. (2013). Oxytocin release response in hand and machine milking Murrah buffaloes. Indian J. Anim. Sci., 83:615-617.
  35. Singh, M.; Dey, R.A. and Sharma, D.K. (2008). Sustainability of Buffalo Dairy Production System in. Peri-Urban Areas of North-East Region: Constraints and Scope of Development. Indian Dairyman, 60: 40- 44. 
  36. Singhal, O.P. and Des Raj (1989). New approaches for chemical quality assurance. Indian Dairyman, 41:43-47. 
  37. Sutherland, M.A., Rogers , A.R., Verkerk, G.A. (2012). The effect of temperament and responsiveness towards humans on the behavior, physiology and milk production of multi-parous dairy cows in a familiar and novel milking environment. Physiol. Behav. , 10:107:329-37.
  38. Thomas, C.S. Svennersten-Sjaunja, K.; Bhosrekar, M.R. and Bruckmaier, R.M. (2004). Mammary cisternal size, cisternal milk and milk ejection in Murrah buffaloes. J. Dairy Res., 71: 162–168. 
  39. Thomas, C.S.; Bruckmaier, R.M.; Östensson, K. and Svennersten, S.K. (2005). Effect of different milking routines on milking-related release of the hormones oxytocin, prolactin and cortisol, and on milk yield and milking performance in Murrah buffaloes. J. Dairy Res., 72: 10-18. 
     

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