Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

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Indian Journal of Animal Research, volume 49 issue 5 (october 2015) : 687-693

Isolation and identification of Mycobacterium bovis and Mycobacterium tuberculosis from animal tissues by conventional and molecular method

Abdul Basit, Mubbashir Hussain, Sultan Ayaz, Muhammad Shahid, Kashif Rahim, Iqbal Ahmad, Riaz Ullah, Abeer Hashem, Elsayed Abd-Allah, Abdulaziz Abdullah Alqarawi, Naila Gul
1Department of Microbiology, Kohat University of Science and Technology (KUST), Khyber Pakhtunkhwa Kohat, 26000, Pakistan.
Cite article:- Basit Abdul, Hussain Mubbashir, Ayaz Sultan, Shahid Muhammad, Rahim Kashif, Ahmad Iqbal, Ullah Riaz, Hashem Abeer, Abd-Allah Elsayed, Alqarawi Abdullah Abdulaziz, Gul Naila (2024). Isolation and identification of Mycobacterium bovis and Mycobacterium tuberculosis from animal tissues by conventional and molecular method. Indian Journal of Animal Research. 49(5): 687-693. doi: 10.18805/ijar.5583.

ABSTRACT

Tuberculosis is a worldwide contagious and chronic disease of human as well as domestic animals with zoonotic potential. The Mycobacterium bovis and Mycobacterium tuberculosis are the main cause of tuberculosis. It has worldwide distribution with significant effect on animals and has public health importance. Therefore the present study was conducted to determine the prevalence of Mycobacterium bovis and Mycobacterium tuberculosis among the ruminant of district Kohat, Khyber PakhtunKhwa and also to evaluate the sensitivity and specificity of Microscopy and PCR. A total 200 tissue samples of lungs, lymph nodes and liver from cattle, buffaloes, sheep and goats were collected from Abattoir Kohat. All the tissue were first examined by direct smear microscopy by Ziehl Neelsen staining and then subjected to the PCR for the detection of M. bovis and M. tuberculosis. The overall prevalence of tuberculosis was 6.5% by PCR.Prevalence of tuberculosis was recorded in7.87% of lungs samples followed by 5.26% lymph node. Moreover the prevalence was found 5.2%, 4%, 10.6% and 6.5% in cattle, buffalos, Goats and sheep respectively. Furthermorethe sensitivity and specificity of PCR and microscopy in term of detection of TB was determined that PCR was found less sensitive then microscopy because of other species which was not amplified due to non availability of specific primer and were found positive in microscopy.In conclusion PCR is more reliable diagnostic tool for diagnosis of bovine tuberculosis. It is recommended that PCR based diagnostic reference laboratory may be established at district level and Tuberculosis awareness campaign must be arranged.

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