Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

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Indian Journal of Animal Research, volume 50 issue 4 (august 2016) : 471-475

Effect of graded concentration of organic Zinc (Zinc glycinate) on antioxidants status and immune response in commercial broilers

K. Sridhar, D. Nagalakshmi*, D. Srinivasa Rao1, S.V. Rama Rao2
1<p>Department of Animal Nutrition, College of Veterinary Science,&nbsp;Sri &nbsp;Venkateswara Veterinary University, Hyderabad- 500 030, India.</p>
Cite article:- Sridhar K., Nagalakshmi* D., Rao1 Srinivasa D., Rao2 Rama S.V. (2016). Effect of graded concentration of organic Zinc (Zinc glycinate) on antioxidants status and immune response in commercial broilers . Indian Journal of Animal Research. 50(4): 471-475. doi: 10.18805/ijar.9594.

One hundred and twenty day old commercial broiler chicks were randomly allotted to 4 dietary groups, with 6 replicates of 5 birds in each and reared for 42 days under uniform management conditions to study the effect of organic zinc (zinc glycinate; Zn-gly) supplemented at lower levels (30, 20 and 10 ppm) on antioxidant status and immune response in comparison to NRC (1994) recommended levels (40 ppm) of Zn supplemented from inorganic source (ZnSO4). The dietary treatments were corn soybean meal basal diet supplemented with 40 ppm Zn from ZnSO4 (inorganic) (control) and 30, 20 and 10 ppm Zn from Zn-gly (organic). Antioxidant enzyme levels and antibody titres against New castle disease (ND) vaccine (humoral immunity) were estimated on 35th d and oxidative stress markers from liver on 42nd d. On 40th d, the cell mediated immunity (CMI) was assessed as cutaneous basophilic hypertrophy to phytohemagglutinin-P (PHA-P).  The lipid peroxidation in haemolysate (P<0.05) lowered in birds with 30 ppm Zn supplemented as Zn-gly compared to 20 and 10 ppm Zn from Zn-gly and 40 ppm Zn from ZnSO4. The Glutathione peroxidase enzyme activity was higher (P<0.05) with 30 ppm Zn as Zn-gly compared to control. The CMI was sgnificantly (P<0.05) high at 30 ppm Zn supplemented as Zn-gly compared to 40 ppm Zn as ZnSO4 and other organic Zn groups. The SOD activity, humoral and cell mediated immune resonse when fed 20 ppm Zn as Zn-gly was comparable to those on 40 ppm Zn as ZnSO4. The results of the study indicated that reducing the level of Zn from inorganic source (40 ppm) to 50 % (20 ppm) supplementation from Zn-gly  can result in comparable immune and antioxidant status in broiler chicks.


  1. Agarwal, A. and Prabhakaran, S.A. (2005). Mechanism, measurement and prevention of oxidative stress in male reproductive physiology. Indian J. Exp. Biol. 43: 963-974.

  2. Ammerman, C.B. (1995). Methods for estimation of mineral bioavailability. Bioavailability of Nutrients for Animals. [CB Ammerman, DH Baker, and AJ Lewis, ed.]. Academic Press, San Diego, CA, pp. 83-94.

  3. Ao, T., Pierce, J.L., Power, R., Pescatore, A.J., Cantor, A.H., Dawson. K.A. and Ford, M.J. (2009). Effect of different forms of zinc and copper on the performance and tissue mineral content of chicks. Poult. Sci. 88: 2171-2175.

  4. Ao, T., Pierce, J.L., Power, R., Dawson, K.A., Pescatore, A.J., Cantor, A.H and Ford, M. J. 2006. Evaluation of Biplex as organic zinc source for chicks. Int. J. Poult. Sci. 5: 808–811.

  5. Ao, T., Pierce, J.L., Power, R., Pescatore, A.J., Cantor, A.H., Dawson. K.A., Ford, M.J. and Paul, M. (2011). Effects of feeding different concentration and forms of zinc on the performance and tissue mineral status of broiler chicks. British Poult. Sci. 52: 466-471.

  6. Balasubramanian, K.A., Manohar, M. and Mathan, V.I. (1988). An unidentified inhibitor of lipid peroxidation in intestinal mucosa. Biochimica. et Biophysica. Acta. 962: 51-8.

  7. Bartlett, J.R. and Smith, M.O. (2003). Effects of different levels of zinc on the performance and immunocompetence of broilers under heat stress. Poult. Sci. 82: 1580-1588. 

  8. Corrier, D.E. and Deloach, J.R. (1990). Evaluation of cell mediated cutaneous basophil hypersensitivity in young chicken by an inter-digital skin test. Poult. Sci. 69: 403-408

  9. Dalle-Donne, I., Rossi, R., Giustarini, D., Milzani, A., and Colombo, R. (2003). Protein carbonyl groups as biomarkers of oxidative stress. Clinica Chimica Act. 329: 23-38.

  10. Duncan, D.B. 1955. Multiple range and multiple F tests. Biometrics. 11: 1-42.

  11. Feng, J., Ma, W.Q., Niu, H.H., Wu, X.M., Wang, Y. and Feng, J. (2010). Effects of zinc glycine chelate on growth, hematological, and immunological characteristics in broilers. Biol. Trace. Elem. Res. 133: 203-211.

  12. Hudson, B.P., Dozier-3, W.A., Wilson, J.L., Sander, J.E. and Ward, T.L. (2004). Reproductive performance and immune status of caged broiler breeder hens provided diets supplemented with either inorganic or organic sources of zinc from hatching to 65 wk of age. J. Appl. Poult. Res. 13: 349-359. 

  13. Kratzer, F. H. and Vohra, P. (1986). Chelate nutrition. CRC, Boca Raton.

  14. Levine, R.L., Garland, D., Oliver, C.N., Amici, A., Climent, I., Lenz, A.G., Ahn, B.W., Shaltiel, S. and Stadtman, E.R. (1990). Determination of carbonyl content in oxidatively modified proteins. Methods in Enzymology. 186: 464-78.

  15. Ma, W., Niu, H., Feng, J., Wang, Y. and Feng, J. (2011). Effect of zinc glycine on oxidative stress, contents of trace elements, and intestinal morphology in broilers. Biol. Trace Elem. Res. 142: 546-556.

  16. Madesh, M. and Balasubramanian, K.A. (1998). Microtiter plate assay for superoxide dismutase using MTT reduction by superoxide. Indian J. Biochem. Biophys. 35: 184-188.

  17. Mannervik, B. and Carlberg, I. (1985). Glutathione Reductase. Methods in Enzymology, 113: 484-490.

  18. Moghaddam, H.N. and Jahanian, R. (2009). Immunological responses of broiler Chicks can be modulated by dietary supplementation of zinc-methionine in place of inorganic zinc Sources. Asian – Aust. J. Anim. Sci. 22: 396 - 403.

  19. Moron, M.S., Depierre, J.W. and Mannervik, B. (1979). Levels of glutathione, glutathione reductase and glutathione S-    transferase activities in rat lung and liver. Biochimica et Biophysica Acta. 582: 67-78.

  20. NRC. (1994). National Research Council, Nutrient requirements of poultry, 9th edition, National Academy Press, Washington.

  21. Osaretin, A.T.E. and Gabriel, A.A. (2009). Effect of zinc deficiency on memory, oxidative stress and blood chemistry in rats. Inter. J. Biol. Chem. Sci. 3: 513-523.

  22. Paglia, D.E. and Valentine, W.N. (1967). Studies on the quantitative and qualitative of erythrocyte glutathion peroxidase. J. Lab. Clinic. Med. 70: 158–168.

  23. Placer, Z.A., Cushman, L.L. and Johnson, B.C. (1966). Estimation of product of lipid peroxidation (Malonyl Dialdehyde) in biochemical systems. Anal. Biochem. 16: 359–364.

  24. Powell, S.R. (2000). The antioxidant properties of zinc. J. Nutr. 130: 1447S-1454S.

  25. Prasad, A.S. (1991). Discovery of human zinc deficiency and studies in an experimental human model. American J. Clinic. Nutr. 53: 403–412.

  26. Rink, L. and Gabriel, P. (2000). Zinc and immune system. Proceedings of Nutrition Society. 59: 541-552.

  27. Saha, A.R., Hadden, E.M. and Hadden, J.N. (1995). Zinc induces thymulin secretion from human thymic epithelial cells in vitro and augments splenocyte and thymocyte responses in vivo. Int. J. Immuno. Pharmacy. 17: 729-733.

  28. Saygili, E.I., Konukoglu, D., Papila, S. and Aksay, T. (2003). Levels of plasma vitamin E, vitamin C, TBARS and cholesterol in male patients with colorectal tumors. Biochemistry (Moscow). 68: 325-328.

  29. Sunder, G.S., Kumar, Ch.V., Panda, A.K., Raju, M.V.L.N. and Rao, S.V.R. (2012). Effect of supplemental organic Zinc and Manganese on broiler performance, bone measures, tissue mineral uptake and immune response at 35 days of age. Indian J. Poult. Sci. 8: 291-298.

  30. Sunil Kumar, B.V., Kumar Ajeet and Kataria Meena (2011). Effect of heat stress in tropical livestock and different strategies for its amelioration. J. Stress Physiology & Biochem. 7: 45-54.

  31. Tate, D.J., Miceli, M.V. and Newsome, D.A. (1997). Zinc induce catalase expression in cultured fetal human retinal pigment epithelial cells. Current Eye Res. l6: 1017-1023.

  32. Wegmann, N.T.G. and Smithies, O. (1966). A simple haemagglutintaion system requiring small amounts of red cells and antibodies transfusion. Philadelphia. 6: 67.

  33. Yamaguchy, S. (1991). The role of SOD an antioxidant. J. National Cancer Inst. 28: 221–232.

  34. Zhao, J., Shirley, R.B., Vazquez-Anon, M., Dibner, J.J., Richards, J.D., Fisher, P., Hampton, T., Christensen, K. D., Allard, J.P. and Giesen, A.F. (2010). Effects of chelated trace minerals on growth performance, breast meat yield, and footpad health in commercial meat broilers. The J. Appl. Poult. Res. 19: 365-372.

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