Indian Journal of Animal Research

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Research Article

Indian Journal of Animal Research, volume 50 issue 4 (august 2016) : 544-550

Characterization of enterovirulent Escherichia coli isolated from yak calves died of diarrhoea in India 

Samiran Bandyopadhyay1, Achintya Mahanti2, Indranil Samanta*2, Subhasis Batabyal2
1<p>National Research Centre on Yak,&nbsp;(ICAR), Dirang-790 101, Arunachal Pradesh, India</p>
Cite article:- Bandyopadhyay1 Samiran, Mahanti2 Achintya, Samanta*2 Indranil, Batabyal2 Subhasis (2016). Characterization of enterovirulent Escherichia coli isolated from yakcalves died of diarrhoea in India . Indian Journal of Animal Research. 50(4): 544-550. doi: 10.18805/ijar.9293.

ABSTRACT

Shiga toxin producing (STEC), enteropathogenic (EPEC) and enterotoxigenic Escherichia coli (ETEC) were isolated from 29 diarrhoeic yak calves from two districts of Arunachal Pradesh, India (West Kameng and Tawang) during 2005 to 2011. The STEC (28) and EPEC (12) isolates belonged to 25 different O serogroups. Among the 28 STEC isolates, 8 (28.5%) isolates carried only stx1, 10 (35.7%) isolates were positive for both stx1 and stx2, 10 (35.7%) isolates carried only stx2. The stx variants such as stx1c, stx2c, stx2d and stx2e were detected in 7 (25%), 9 (32.1%), 1 (3.5%) and 4 (14.28%) isolates, respectively. Among the 12 EPEC isolates, 2 (4.65%) strains were ‘typical’ possessing bfpA gene. The ehxA and saa genes were present in 19 and 4 isolates, respectively. Among the 14 ETEC isolates, 6 (42.8%), 7 (50%), 5 (35.7%), 5 (35.7%), 7 (50%) and 6 (42.8%) isolates carried genes coding for STa, STb, LT, F5, F41 and EAST1, respectively. The present study detected LT, STb, F41 as the most prevalent type in yaks associated with diarrhoea. Further, the STEC, EPEC and ETEC isolates showed resistance against furazolidone (40%), nitrofurantoin (39%), nalidixic acid (38%), erythromycin (38%), kanamycin (38%), amikacin (37%). The dendrogram revealed clonal relationship among the isolates after RAPD/ERIC analysis. The study could not detect any specific RAPD/ERIC cluster associated with origin, serotypes or virotypes of the isolates. Thus, it was concluded that genetically diverse, multi-drug resistant enterovirulent E. coli were associated with death due to diarrhoea in yak calves in India. 

REFERENCES


  1. Ansaruzzaman, M., Bhuiyan, N.A., Begum, Y.A., Kühn, I., Nair, G.B., Sack, D.A., Svennerholm, A.M., and Qadri, F. (2007). Characterization of enterotoxigenic Escherichia coli from diarrhoeal patients in Bangladesh using phenotyping and genetic profiling. J Med Microbiol 56:217-222.

  2. Bandyopadhyay, S., Mahanti, A., Samanta, I., Datta, T.K., Ghosh, M.K., Bera, A.K., Bandyopadhyay, S., and Bhattacharyya, D. (2011). Virulence repertoire of Shiga toxin-producing Escherichia coli (STEC) and Enterotoxigenic Escherichia coli (ETEC) from diarrhoeic lambs of Arunachal Pradesh, India. Trop Anim Hlth Prod 43:705-710.

  3. Bandyopadhyay, S., Lodh, C., Sarkar, M., Ghosh, M.K., Bera, A.K., Bhattacharyya, D., Mondal, D.K., and Baruah, K.K. (2012). Prevalence, molecular fingerprinting and drug resistance profile of enterovirulent Escherichia coli isolates from free-ranging yaks of Tawang district, Arunachal Pradesh, India. Trop Anim Hlth Prod 44:1063-72.

  4. Bandyopadhyay, S., Lodh, C., Rahaman, H., Bhattacharya, D., Bera, A.K., Ahmed, F.A., Mahanti, A., Samanta, I., Mondal, D.K., Bandyopadhyay, S., Sarkar, S., Dutta, T.K., Maity, S., Paul, V., Ghosh, M.K., Sarkar, M., and Baruah, K.K. (2012a).Characterization of shiga toxin producing (STEC) and enteropathogenic Escherichia coli (EPEC) in raw yak (Poephagus grunniens) milk and milk products. Res Vet Sci 93:604-610.

  5. Bhan, M.K., Khoshoo, V., Sommerfelt, H., Raj, P., Sazawal, S., and Srivastava, R. (1989). Enteroaggregative Escherichia coli and Salmonella associated with nondysenteric persistent diarrhea. Pediatr Infect Dis J 8:499-502.

  6. Cergole-Novella, M.C., Nishimura, L.S., Dos Santos, L.F., Irino, K., Vaz, T.M., Bergamini, A.M., and Guth, B.E. (2007). Distribution of virulence profiles related to new toxins and putative adhesins in Shiga toxin-producing Escherichia coli isolated from diverse sources in Brazil. FEMS Microbiol Lett 274:329-334.

  7. Clinical and Laboratory Standards Institute (CLSI). (2008). Performance standards for antimicrobial disk and dilution susceptibility tests for bacteria isolated from animals, third ed. Approved Standard. CLSI document M31-A3. Clinical and Laboratory Standards Institute, Wayne, PA.

  8. Enne, V.I., Cassar, C., Sprigings, K., Woodward, M.J., and Bennett, P.M. (2008). A high prevalence of antimicrobial resistant Escherichia coli isolated from pigs and a low prevalence of antimicrobial resistant E. coli from cattle and sheep in Great Britain at slaughter. FEMS Microbiol Lett 278:193-199.

  9. Francis, D.H. (2002). Enterotoxigenic Escherichia coli infection in pigs and its diagnosis. J Swine Hlth Prod 10:171-175.

  10. Islam, M.A., Heuvelink, A.E., de Boer, E., Sturm, P.D., Beumer, R.R., Zwietering, M.H., Faruque, A.S., Haque, R., Sack, D.A., and Talukder, K.A. (2007). Shiga toxin-producing Escherichia coli isolated from patients with diarrhoea in Bangladesh. J Med Microbiol 56:380-385.

  11. Kim, Y.J., Kim, J.H., Hur, J., and Lee, J.H. (2010). Isolation of Escherichia coli from piglets in South Korea with diarrhea and characteristics of the virulence genes. Can J Vet Res 74:59-64.

  12. Lee, J.H. (2009). Antimicrobial resistance of Escherichia coli O26 and O111 isolates from cattle and their characteristics. Vet Microbiol 135:401-405.

  13. Mahanti, A., Samanta, I., Bandopaddhay, S., Joardar, S.N., Dutta, T.K., Batabyal, S., Sar, T.K., and Isore, D.P. (2013). Isolation, molecular characterization and antibiotic resistance of Shiga Toxin-Producing Escherichia coli (STEC) from buffalo in India. Lett Appl Microbiol 56:291-298.

  14. Paton, J.C., and Paton, A.W. (1998). Pathogenesis and diagnosis of Shiga toxin producing Escherichia coli infections. Clin Microbiol Rev 11:450–479.

  15. Prabhu, V., Isloor, S., Balu, M., Suryanarayana, V.V.S., and Rathanama, D. (2010). Genotyping by ERIC-PCR Escherichia coli isolated from bovine mastitis cases. Indian J Biotechnol 9:298-301.

  16. Pradel, N., Bertin, Y., Martin, C., and Livrelli, V. (2008). Molecular analysis of shiga toxin-producing Escherichia coli strains isolated from hemolytic-uremic syndrome patients and dairy samples in France. Appl Microbiol Biotechnol 74:2118-2128.

  17. Samanta, I., Joardar, S.N., Das, P.K., Das, P., Sar, T.K., Dutta, T.K., Bandyopadhyay, S., Batabyal, S., and Isore, D.P. (2013). Virulence repertoire, characterization and antibiotic resistance pattern analysis of Escherichia coli isolated from backyard layers and their environment in India. Avian Dis 58:39-45. 

  18. Toma, C., Martínez Espinosa, E., Song, T., Miliwebsky, E., Chinen, I., Iyoda, S., Iwanaga, M., and Rivas, M. (2004). Distribution of putative adhesins in different seropathotypes of Shiga toxin-producing Escherichia coli. J Clin Microbiol 42:4937-4946.

  19. Veilleux, S., and Dubreuil, J.D. (2006). Presence of Escherichia coli carrying the EAST1 toxin gene in farm animals. Vet Res 37:03-13.

  20. Wales, A.D., Woodward, M.J., and Pearson, G.R. (2005). Attaching-effacing bacteria in animals. J Comp Pathol 132:1-26.

  21. Wang, G., Clark, C.G., and Rodgers, F.G. (2002). Detection in Escherichia coli of the genes encoding the major virulence factors, the genes defining the O157:H7 serotype, and components of the type 2 Shiga Toxin Family by multiplex PCR. J Clin Microbiol 40:3613-3619.

  22. Wani, S.A., Hussain, I., Nabi, A., Fayaz, I., and Nishikawa, Y. (2007). Variants of eae and stx genes of atypical enteropathogenic Escherichia coli and non-O157 Shiga toxin-producing Escherichia coli from calves. Lett Appl Microbiol 45:610-615.

  23. Wieler, L.H., Sobjinski, G., Schlapp, T., Failing, K., Weiss, R., Menge, C., and Baljer, G. (2007). Longitudinal prevalence study of diarrheagenic Escherichia coli in dairy calves. Berlin Munchen tierarztliche Wochens 120: 296-306.

     
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