Cysteamine and b-mercaptoethanol supplementation improves the in vitro embryo development in buffaloes (Bubalus bubalis)

DOI: 10.18805/ijar.9490    | Article Id: B-2878 | Page : 305-309
Citation :- Cysteamine and b-mercaptoethanol supplementation improves the in vitroembryo development in buffaloes (Bubalus bubalis) .Indian Journal Of Animal Research.2016.(50):305-309

Vijay Singh1, A.K. Misra2, Suresh Kumar*3 and Champak Barman4

suresh_vet079@rediffmail.com
Address :

Department of Animal Reproduction, Gynaecology & Obstetrics, College of Veterinary and Animal Science, G.B.P.U.A. & T., Pantnagar-263 145, India.

Submitted Date : 1-07-2014
Accepted Date : 23-02-2016

Abstract

The objective of the present experiment was to investigate the effect of cysteamine and b-mercaptoethanol supplementation on in -vitro maturation, cleavage of oocytes and development of embryo in buffalo (Bubalus bubalis). Oocytes were aspirated from abattoir ovarian follicles of 3-10 mm diameter followed by maturation in the media in vitro containing cysteamine/b-mercaptoethanol (treatment) and without antioxidant (control). Matured oocytes were co-incubated with sperm (approx.1×106/ml) of Murrah bull in mSOF medium using heparin (10 µg/ml). After 22 h of oocyte-sperm incubation, fertilized oocytes were stripped of cumulus cells and cultured in mSOF medium for 8 days to study embryo development. The oocyte maturation rate improved significantly (P<0.05) following addition of  50 or 100 µM of cysteamine and 10, 50 and 100 µM of  b- mercaptoethanol (ME), respectively as compared to control. The cleavage rate was found to be significantly (P<0.05) higher at 50 and 100 µM of cysteamine and at all concentrations of b-mercaptoethanol as compared to control and development of embryos to morula stage was significantly (P<0.05) improved with 50 µM cysteamine/ b-mercaptoethanol.
 

Keywords

b-mercaptoethanol Cleavage Cysteamine Embryo In vitro maturation.

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