Indian Journal of Animal Research

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Research Article

Indian Journal of Animal Research, volume 50 issue 3 (june 2016) : 305-309

Cysteamine and b-mercaptoethanol supplementation improves the in vitro embryo development in buffaloes (Bubalus bubalis)

Vijay Singh1, A.K. Misra2, Suresh Kumar*3, Champak Barman4
1<p>Department of Animal Reproduction, Gynaecology &amp; Obstetrics,&nbsp;College of Veterinary and Animal Science, G.B.P.U.A. &amp; T., Pantnagar-263 145, India.</p>
Cite article:- Singh1 Vijay, Misra2 A.K., Kumar*3 Suresh, Barman4 Champak (2016). Cysteamine and b-mercaptoethanol supplementation improves the in vitroembryo development in buffaloes (Bubalus bubalis) . Indian Journal of Animal Research. 50(3): 305-309. doi: 10.18805/ijar.9490.

ABSTRACT

The objective of the present experiment was to investigate the effect of cysteamine and b-mercaptoethanol supplementation on in -vitro maturation, cleavage of oocytes and development of embryo in buffalo (Bubalus bubalis). Oocytes were aspirated from abattoir ovarian follicles of 3-10 mm diameter followed by maturation in the media in vitro containing cysteamine/b-mercaptoethanol (treatment) and without antioxidant (control). Matured oocytes were co-incubated with sperm (approx.1×106/ml) of Murrah bull in mSOF medium using heparin (10 µg/ml). After 22 h of oocyte-sperm incubation, fertilized oocytes were stripped of cumulus cells and cultured in mSOF medium for 8 days to study embryo development. The oocyte maturation rate improved significantly (P<0.05) following addition of  50 or 100 µM of cysteamine and 10, 50 and 100 µM of  b- mercaptoethanol (ME), respectively as compared to control. The cleavage rate was found to be significantly (P<0.05) higher at 50 and 100 µM of cysteamine and at all concentrations of b-mercaptoethanol as compared to control and development of embryos to morula stage was significantly (P<0.05) improved with 50 µM cysteamine/ b-mercaptoethanol.

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