Banner
Current IssueEditorial BoardOnline First Articles
Current IssueEditorial BoardOnline First Articles
Indian Journal of
Animal Research
Print ISSN: 0367-6722
Online ISSN: 0976-0555
NASS Rating
6.40
SJR
0.233
CiteScore
0.606

Chief Editor:
M. R. Saseendranath
Kerala Veterinary and Animal Science University, Mannuthy, Thrissur, INDIA
Frequency:Monthly
Indexing:
Science Citation Index Expanded, BIOSIS Preview, ISI Citation Index, Biological Abstracts, Scopus, ...

Indian Journal of Animal Research, volume 49 issue 5 (october 2015) : 694-698

Detection and virulence gene characterization of Shiga toxigenic E. coli from buffalo meat samples

Neha Sinha, D.P. Kshirsagar, M.N. Brahmbhatt, J.B. Nayak
1Department of Veterinary Public Health and Epidemiology, Veterinary College, Anand Agricultural University, Anand-388 001, India.
Cite article:- Sinha Neha, Kshirsagar D.P., Brahmbhatt M.N., Nayak J.B. (2025). Detection and virulence gene characterization of Shiga toxigenic E. coli from buffalo meat samples. Indian Journal of Animal Research. 49(5): 694-698. doi: 10.18805/ijar.5584.

ABSTRACT

Shiga-toxigenic Escherichia coli (STEC) are causative agents of bloody diarrhoea, haemorrhagic colitis (HC) and haemolytic uraemic syndrome (HUS). Humans acquire infections primarily through contaminated beef. In India, STEC has not been implicated as a major cause of diarrhoea. The study was carried out to know the prevalence of shiga toxin (ST) producing Escherichia coli (STEC) and virulence genes characterization from buffalo meat samples sold at various retail meat shops at Anand, India. In the present investigation 70 isolates of Escherichia coli isolated from 150 retail market buffalo meat samples, were screened for STEC, using conventional culture methods, serotyping and polymerase chain reaction (PCR). Out of 70 E. coli isolates 11 different ‘O’ serogroups were recorded in 44 isolates. While 19 isolates were untypable and seven were rough isolates. Out of the 70 E. coli tested 56 isolates (80%) were positive for stx genes: of which 51 (72.85%) harboured stx2 genes and 2 isolates (2.85%) were positive for stx1 gene only. Moreover, 3 E. coli isolates (4.28%) harboured both stx1 and stx2. About 66 (94.28%) isolates were positive for eaeA gene. While out of 70 E. coli isolates tested, 3 (4.28%) were found to be positive for rfb O157 gene. Presence of STEC and other virulence factors in buffalo meat samples appeared to be matter of concern and threat to public health.

REFERENCES

  1. Arya, G., Roy, A., Choudhary, V., Yadav, M.M. and Joshi, C.G. (2008). Serogroups, atypical biochemical characters, colicinogeny and antibiotic resistance pattern of shiga toxin-producing Escherichia coli isolated from diarrhoeic calves in Gujarat, India. Zoonoses Public Health. 55:89–98.
  2. Blanco, M., Blanco, J.E., Mora, A., Rey, J., Alonso,J. M., Hermoso, M., Hermoso, J., Alonso, M.P., Dahbi, G., Gonza´lez, V.M., Bernardez, I. and Blanco, J. (2003). Serotypes, Virulence Genes, and Intimin Types of Shiga Toxin (Verotoxin)- Producing Escherichia coli Isolates from Healthy Sheep in Spain. J. of Clinic. microbiol. 41(4): 1351–1356.
  3. Blanco, M., Padola, N.L., Kruger, A., Sanz, M.E., Blanco, J.E., Gozalez, E.A., Dahbi, G., Mora, A., Bernardez, M.I., Etcheverria, A.I., Arroyo, G.H., Lucchesi, P.M.A., Parma, A.E. and Blanco, J. (2004). Virulence genes and intimin types of Shiga-toxin-producing Escherichia coli isolated from cattle and beef products in Argentina. Int. Microbiol.7: 269-276.
  4. Brando, R.J., Miliwebsky, E., Bentancor, L., Deza, N., Baschkier, A., Ramos, M.V., Fernández, G.C., Meiss, R., Rivas, M., Palermo, M.S. (2008). Renal damage and death in weaned mice after oral infection with Shiga toxin 2-producing Escherichia coli strains. Clin Exp Immunol.153:297-306.
  5. Chattopadhyay, U.K., Gupta, S. and Dutta, S. (2003). Search for Shiga toxin producing Escherichia coli (STEC) including O157:H7 strains in and around Kolkata. Indian J. Med. Microbiol.21: 17-20.
  6. Dhanashree, B. and Mallya, P.S. (2008). Detection of shiga-toxigenic Escherichia coli (STEC) in diarrhoeagenic stool & meat samples in Mangalore, India. Indian J Med Res.128: 271-277Paton, J.C. and Paton, A.W. 1998. Pathogenesis and diagnosis of Shiga toxin-producing Escherichia coli infections. Clin. Microbiol. Rev.11: 450–479.
  7. Galland, J.C., Hyatt, D.R., Crupper, S.S. and Acheson, D.W. (2001). Prevalence, antibiotic susceptibility, and diversity of Escherichia coli O157:H7 isolates from a longitudinal study of beef cattle feedlots. Appl. Environ. Microbiol. 67(4): 1619-1627.
  8. Hazarika, R.A., Singh, D.K., Kapoor, K.N., Agrawal, R.K., Pandey, A.B. and Purosottam (2007). Verotoxigenic Escherchia coli (STEC) from beef and its products. Ind. J. of Experimen. Biology.45:207-211.
  9. Hitchins, A.D., Feng, P., Watkins, W.D., Rippey, S.R. and Chandler, L.A. (2001). E. coli and the coliform bacteria. In Bacteriological Analytical Manual, Chapter 4, 8th edn, Revision A. Gaithersburg, MD: U.S. Food and Drug Administration, AOAC International.
  10. Irino, K., Kato, M.A.M.F., Vaz, T.M.I., Ramo, I.I., Souza, M.A.C., Cruz, A.S., Gomes, T.A.T., Vieira, M.A.M. and Gath, B.E.C. (2005). Serotypes and virulence markers of shiga toxin producing E. coli (STEC) isolated from dairy cattle in Sau Paulo State, Brazil. Vet. Microbiol. 105: 29-36.
  11. Karmali, M.A., Masarenhas, M., Shen, S., Ziebell, K.,Johnson, S., Reid Smith, R., Isaac-Renton, J., Clark, C., Rahn, K and Kaper, J.B. (2003). Association of genomic O island 122 of Escherichia coli EDL 933 with vero cytotoxin-producing Escherichia coli seropathotypes that are linked to epidemic and/ or serious disease. J. Clin. Microbiol. 41(11): 4930-4940.
  12. Kobayashi, H., Shimada, J., Nakazawa, M., Morozumi, T., Pohjanvirta, T, Pelkonen, S. and Yamamoto, K. (2001). Prevalence and characteristics of shiga toxin-producing Escherichia coli from healthy cattle in Japan. Appl. Environ. Microbiol.67: 484-489.
  13. Milnes, A.S., Stewart, I., Clifton-Hadley, F.A., Davies, R.H., Newell, D.G., Sayers, A.R., Cheasty, T., Cassar, C., Ridley, A., Cook, A.J.C., Evans, S.J., Teale, C.J., Smith, R.P., McNally, A., Toszeghy, M., Futter, R., Kay, A. and Paiba, G.A. (2008). Intestinal carriage of verocytotoxigenic Escherichia coli O157, Salmonella, thermophilic Campylobacter and Yersinia enterocolitica, in cattle, sheep and pigs at slaughter in Great Britain during 2003. Epidemiol. Infect.136:739–751.
  14. Mora, A., Blanco, M., Blanco, J.E., Dahbi, G., López, C., Justel, P., Alonso, M.P., Echeita, A., Bernárdez, M.I., González, E.A. and Blanco, J. (2007). Serotypes, virulence genes and intimin types of Shiga toxin (verocytotoxin)-producing Escherichia coli isolates from minced beef in Lugo (Spain) from 1995 through 2003. BMC Microbiology 7:13.
  15. Nataro, J.P., and Kaper, J.B. (1998). Diarrheagenic Escherichia coli. J. Clin. Microbiol. 11: 142–201.
  16. Osek, J. (2004). Phenotypic and genotypic characterization of Escherichia coli O157 strains isolated from humans, cattle and pigs. Vet. Med. – Czech. 49(9): 317–326.
  17. Rahman, H. (2002). Prevalence of verotoxin genes among non-enterohaemorrhagic Escherichia coli Indian J. Comp. Microbiol. Immunol. Infect. Dis.23: 189-190.
  18. Renter, D.G., Smith, D.R., King, R., Stilborn, R., Berg, J., Berezowaski, J. and McFall, M. (2008). Detection and determinants of Escherichia coli O157:H7 in Alberta feedlot pens immediately prior to slaughter. The Canad. J. of Veterinary Research. 72:217-227
  19. Salnec, T., Fruth, A., Cruzburg, K. and Schmidth, H. (2009). Molecular analysis of virulence profile and shiga toxin genes in food borne shiga toxin producing E. coli. App. Environ. Microbiol.75 (19): 6187- 6197.
  20. Wani, S.A., Bhat, M.A., Samanta, I., Nishikawa, Y. and Buchh, A.S. (2003). Isolation and characterization of Shiga toxin- producing Escherichia coli (STEC) and enteropathogenic Escherichia coli (EPEC) from calves and lambs with diarrhoea in India. Appl. Microbio. 37: 121–126.
  21. Zweifel, C., Schumacker, S., Blanco, M., Blanco, J.E., Tasara, T., Blanco, J. and Stephen, R. (2005). Phenotypic and genotypic characteristics of non-O157 Shiga toxin- producing E. coli (STEC) from Swiss Cattle. Vet. Microbiol. 105: 37-45.
Reviewed By

Download Article
In this Article
    Contact us
    Follow us

    Editorial Board

    View all (0)