Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

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Indian Journal of Animal Research, volume 50 issue 3 (june 2016) : 411-414

Immunomodulatory effect of levamisole on PPR vaccine in goats and change in haematological profile

Mousam Das*, D.P. Isore, S.N. Joardar, I. Samanta, S.K. Mukhopadhayay
1<p>Department of Veterinary Microbiology, West Bengal University of Animal &amp; Fishery Sciences, Kolkata-700 037, India.</p>
Cite article:- Das* Mousam, Isore D.P., Joardar S.N., Samanta I., Mukhopadhayay S.K. (2015). Immunomodulatory effect of levamisole on PPR vaccine in goats and change in haematological profile . Indian Journal of Animal Research. 50(3): 411-414. doi: 10.18805/ijar.5723.

Levamisole is an anthelmintic and synthetic immunomodulator. Effect of levamisole on PPR vaccine in goat model was evaluated in this study. Healthy, sero negative for PPR infection 12 Black Bengal goats were divided into three groups. Levamisole was injected @ 2.5 mg/kg b.wt. and @ 5.0 mg/kg b.wt. in two groups respectively. After seven days all animals were vaccinated with PPR cell culture vaccine. Blood samples were collected from animals at 0 day, 7th day, 14th day, 21st day and 30th day. Serum was separated and used in indirect ELISA for qualitative and quantitative assay of antibody titer. EDTA mixed blood sample was used for determination of immunity induced blood cells number. Antibody titer and immunity induced blood cells number were more in the Levamisole and vaccine treated groups than only vaccine treated group. So, levamisole acted as an immunomodulator when it was used prior PPR vaccine in goats.


  1. Abu Elzein, E.M.E., Hassanien, M.M., Al Afaleq, A.I., Elhadi, M.A. and Housawi, F.M.I. (1990). Isolation of peste des petits ruminants from goats in Saudi Arabia. Vet. Rec., 127: 309-310.

  2. Anderson, D.P. (1992). Immunostimulants, adjuvants, and vaccine carriers in fish applications to aquaculture. Annual Review of Fish Diseases. 2: 281-307.

  3. Babiuk, L.A. and Misra, V. (1981). Levamisole and bovine immunity: in vitro and in vivo effects on immune responses to herpes virus immunization. Canadian Journal of Microbiology. 27: 1312–1319.

  4. Balamurugan, V., Singh, R.P., Saravanan, P., Sen, A., Sarkar, J., Sahay, B., Rasool, T.J. and Singh, R.K. (2007). Development of an indirect ELISA for the detection of antibodies against Peste-des-petits-ruminants virus in small ruminants. Veterinary Research Communications. 31: 355-364.

  5. Brunner, C.J. and Muscoplat, C.C. (1980). Immunomodulatory effect of levamisole. Journal of American Veterinary Medical Association. 176: 1159–1162.

  6. Intizar, M., Ahmad, M.D., Anjum, A.A. and Hanif, A. (2009). Comperative efficacy of peste des petits ruminants (PPR) vaccines available in Pakistan in sheep and goats. Pakistan. Vet. J. 29: 202-205.

  7. Jin, H., Li, Y., Ma, Z., Zhang, F., Xie, Q., Gu, D. and Wang, B. (2004). Effect of chemical adjuvants on DNA vaccination. Vaccine. 22: 2925– 2935.

  8. Joshi, V.B., Nagal, K.B., Sarma, M., Katoch, R.C., Batta, M.K. and Sharma, A.K. (1996). Peste des petits ruminants (PPR) among Gaddi sheep and goats in Himachal Pradesh. Indian. J. Anim. Sci. 66: 1126-1127.

  9. Lejan, C. and Asso, J. (1981). Induction of immunity in calves. In: Current Therapy in Theriogenology. (Morrow, D.A. eds), WB Saunders Company, Philadelphia, pp 224-226. .

  10. Maqsood, S., Samoon, M.H. and Singh, P. (2009). Immunomodulatory and Growth Promoting Effect of Dietary Levamisole in Cyprinus carpio Fingerlings Against the Challenge of Aeromonas hydrophila. Turkish Journal of Fisheries and Aquatic Sciences. 9: 111-120.

  11. Mojzisova, J., Hromada, R., Paulik, S., Ondrasovic, M. and Bajova, V. (2004). Immune response and immunomodulatory effect of levamisole in immunosuppressed dogs vaccinated against Parvo virus. Bulletin of Veterinary Institute in Pulawy. 48: 93–97.

  12. Pelletier, M., Willoughby, D.A. and Giround, J.P. (1978). Modulating effect of levamisole on DNA synthesis in macrophages in vitro. Journal of the Reticuloendothelial Society. 24: 333–338.

  13. Raa, J. (1996). The role of immunostimulatory substances in fish and shell fish farming. Reviews in Fishery Sciences. 4: 229-288.

  14. Renoux, G. (1978). Modulation of immunity by levamisole. Pliaritta Tfiera. 2: 397-423.

  15. Sabry, E.O., Arafa, A.S., Ali, M.M.A. and Lamya, A.F.A. (2010). Immunomodulator effect of levamisole on broilers vaccinated with avian influenza vaccine (H5N1). 3rd Animal Wealth Research Conference in the Middle East & North Africa, Egypt, pp 384-395. 

  16. Schalm, O.W. (1958). The leukocytes. Calif. Vet. 12: 18-19.

  17. Singh, R.P., De, U.K. and Pandey, K.D. (2010). Virological and antigenic characterization of two peste des petits ruminants (PPR) vaccine viruses of Indian origin. Com. Immunol. Microbiol. Infect. Dis. 33: 343-353.

  18. Stelletta, C., Cuteri, V., Bonizzi, L., Frangipane di Regalbono, A., Orsi, F., Nisoli, L., Lulla, D. and Morgante, M. (2004). Effect of levamisole administration on bluetongue vaccination in sheep. Veterinaria Italiana. 40: 635-639.

  19. Sreenivasa, B.P., Dhar, P., Singh, R.P. and Bandyopadhyay, S.K. (2000). Evaluation of an indigenously developed homologous live attenuated cell culture vaccine against Peste-des-petits-ruminants infection of small ruminants. Proceedings of XX Annual Conference of Indian Association of Veterinary Microbiologists, Immunologists and Specialists in Infectious Diseases (IAVMI), Pantnagar, Uttaranchal, India. 84.

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